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Antagonism between novobiocin and coumermycin A1 in Bacillus subtilis

Published online by Cambridge University Press:  14 April 2009

István Gadó ,
Valéria Széll ,
Kálmán Büki  and
György Szvoboda
István Gadó
Affiliation:
Institute for Drug Research, Budapest, Hungary;
Valéria Széll
Affiliation:
Institute for Drug Research, Budapest, Hungary;
Kálmán Büki
Affiliation:
Second Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary
György Szvoboda
Affiliation:
Institute for Drug Research, Budapest, Hungary;
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Summary

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When combinations of inhibitors acting on the subunit B of DNA gyrase were tested in Bac. subtilis strains, the growth-inhibiting effect of novobiocin was specifically antagonized by subinhibitory concentrations of coumermycin A1. An antagonism in the opposite direction was not observed.

Two alternative models are proposed, where the supercoiling decrease caused by novobiocin is antagonized by coumermycin.

This phenomenon seems to be characteristic of the Bac. subtilis species.

Type
Research Article
Information
Genetics Research , Volume 44 , Issue 3 , December 1984 , pp. 243 - 250
Copyright
Copyright © Cambridge University Press 1984

References

REFERENCES

Chao, L. (1978). An unusual interaction between the target of nalidixic acid and novobiocin. Nature 271, 385–386.CrossRefGoogle ScholarPubMed
Cozzarelli, N. R. (1980). DNA gyrase and the supercoiling of DNA. Science 207, 953–960.CrossRefGoogle ScholarPubMed
Gadó, I., Kari, Cs., Széll, V. & Szvoboda, Gy. (1982). Novel pleiotropic effect of rifampicin resistance in a Micromonospora sp. Genetical Research 40, 33–40.CrossRefGoogle Scholar
Garrett, E. R. & Won, C. M. (1973). Effect of novobiocin and its combination with tetracycline, chloramphenicol, erythromycin and lincomycin on the microbial generation of Escherichia coli. Antimicrobial Agents and Chemotherapy 4, 626–633.CrossRefGoogle ScholarPubMed
Gellert, M. (1981). DNA topoisomerases. Annual Review of Biochemistry 50, 879–910.CrossRefGoogle ScholarPubMed
Gellert, M., Mizuuchi, K., O'Dea, M. H. & Nash, H. A. (1976 a). DNA gyrase: an enzyme that introduces superhelical turns into DNA. Proceedings of the National Academy of Sciences of the USA 73, 3872–3876.CrossRefGoogle ScholarPubMed
Gellert, M., O'Dea, M. H., Itoh, T. & Tomizawa, J. (1976 b). Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proceedings of the National Academy of Sciences of the USA 73, 4474–4478.CrossRefGoogle ScholarPubMed
Hansen, F. G. & Von Meyenburg, K. (1979). Characterization of the dnaA, gyrB and other genes in the dnaA region of Escherichia coli chromosome on specialized tranducing phages ∧tna. Molecular and General Genetics 175, 135–144.CrossRefGoogle Scholar
Higgins, N. P., Peebles, C. L., Sugino, A. & Cozzarelli, N. R. (1978). Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymie activity. Proceedings of the National Academy of Sciences of the USA 75, 1773–1777.CrossRefGoogle ScholarPubMed
Mizuuchi, K., O'Dea, M. H. & Gellert, M. (1978). DNA gyrase: subunit structure and ATPase activity of the purified enzyme. Proceedings of the National Academy of Sciences of the USA 75, 5960–5963.CrossRefGoogle ScholarPubMed
Orr, E. & Staudenbauer, W. L. (1982). Bacillus subtilis DNA gyrase: purification of subunits and reconstitution of supercoiling activity. Journal of Bacteriology 151, 524–527.CrossRefGoogle ScholarPubMed
Ryan, M. J. & Wells, R. D. (1976). Coumermycin A1: a preferential inhibitor of replicative DNA synthesis in Escherichia coli. II. In vitro characterization. Biochemistry 15, 3778–3782.CrossRefGoogle Scholar
Sugino, A. & Bott, K. F. (1980). Bacillus subtilis deoxyribonucleic acid gyrase. Journal of Bacteriology 141, 1331–1339.CrossRefGoogle ScholarPubMed
Sugino, A., Higgins, N. P., Brown, P. O., Peebles, C. L. & Cozzarelli, N. R. (1978). Energy coupling in DNA gyrase and the mechanism of action of novobiocin. Proceedings of the National Academy of Sciences of the USA 75, 4838–4842.CrossRefGoogle ScholarPubMed
Sugino, A., Peebles, C. L., Kreuzer, K. N. & Cozzarelli, N. R. (1977). Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme. Proceedings of the National Academy of Sciences of the USA 74, 4767–4771.CrossRefGoogle Scholar