Hostname: page-component-76c49bb84f-lvrpm Total loading time: 0 Render date: 2025-07-12T14:00:00.326Z Has data issue: false hasContentIssue false
  • English
  • Français

Combined chromosomal and plasmid encoded control for the degradation of phenol in Pseudomonas putida

Published online by Cambridge University Press:  14 April 2009

C. L. Wong  and
N. W. Dunn
C. L. Wong
Affiliation:
School of Biological Technology, University of New South Wales, P.O. Box 1, Kensington, New South Wales, 2033, Australia
N. W. Dunn
Affiliation:
School of Biological Technology, University of New South Wales, P.O. Box 1, Kensington, New South Wales, 2033, Australia
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The TOL(M1) metabolic plasmid was transferred from Pseudomonas arvilla mt-2 to a mutant of Pseudomonas putida. Although neither the donor nor the recipient could grow on phenol, the transconjugants could grow slowly on this carbon source. In these transconjugants phenol was converted to catechol by chromosomal encoded phenol hydroxylase followed by degradation of catechol by low uninduced levels of the plasmid encoded catechol meta cleavage pathway. A mutant, which grew well on phenol, was isolated from one of the transconjugants and it was found that phenol could now act as an inducer for the meta cleavage pathway.

Information

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 3 , June 1976 , pp. 405 - 412
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Chakrabarty, A. M. (1972). Genetic basis of the biodegradation of salicylate in Pseudomonas. Journal of Bacteriology 112, 815–823.CrossRefGoogle ScholarPubMed
Dunn, N. W. & Gunsalus, I. C. (1973). Transmissible plasmid coding early enzymes of naphthalene oxidation in Pseudomonas putida. Journal of Bacteriology 114, 974–979.CrossRefGoogle ScholarPubMed
Feist, C. F. & Hegeman, G. D. (1969). Phenol and benzoate metabolism by Pseudomonas putida: regulation of tangential pathways. Journal of Bacteriology 100, 869–877.CrossRefGoogle ScholarPubMed
Sala-Trepat, J. M., Murray, K. & Williams, P. A. (1972). The metabolic divergence in the meta cleavage of catechols by Pseudomonas putida NC1B 10015. European Journal of Biochemistry 28, 347–356.CrossRefGoogle Scholar
Williams, P. A. & Murray, K. (1974). Metabolism of benzoate and the methyl benzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of the TOL plasmid. Journal of Bacteriology 120, 416–423.CrossRefGoogle Scholar
Wong, C. L. & Dunn, N. W. (1974). Transmissible plasmid coding for the degradation of benzoate and m-toluate in Pseudomonas arvilla mt-2. Genetical Research, Cambridge 23, 227–232.CrossRefGoogle ScholarPubMed