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Derepression of F-lac in Salmonella typhimurium by a determinant for kanamycin resistance

Published online by Cambridge University Press:  14 April 2009

H. R. Smith ,
June N. Grindley ,
N. D. F. Grindley  and
E. S. Anderson
H. R. Smith
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
June N. Grindley
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
N. D. F. Grindley
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9
E. S. Anderson
Affiliation:
Enteric Reference Laboratory, Public Health Laboratory Service, Colindale Avenue, London, N.W.9

Summary

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When a determinant for neomycin-kanamycin resistance (K) was transferred by an F-lac factor into Salmonella typhimurium, the resulting KF-lac strain was sensitive to the male-specific phage μ2 and F-lac was derepressed. However, F-lac alone is repressed in S. typhimurium. When kanamycin resistance is spontaneously lost from S. typhimurium KF-lac an element persists which derepresses F-lac in S. typhimurium. The results are consistent with the hypothesis that a locus der, for derepression of F-lac in S. typhimurium, lies on the K plasmid. The R factor Rldrd19 is derepressed in K12 but is repressed in S. typhimurium. It also is derepressed by der. In contrast to F-lac and R1, another R factor, R136drdH8, is derepressed in both K12 and S. typhimurium, so that the intervention of der is unnecessary for its derepression in the salmonella host.

Type
Short Papers
Information
Genetics Research , Volume 16 , Issue 3 , December 1970 , pp. 349 - 353
Copyright
Copyright © Cambridge University Press 1970

References

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