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Differential inhibition of the initiation of DNA replication in stringent and relaxed strains of Escherichia coli

Published online by Cambridge University Press:  14 April 2009

Elena C. Guzman ,
Francisco J. Carrillo  and
Alfonso Jimenez-Sanchez
Elena C. Guzman
Affiliation:
Departamento de Bioquimica y Biologia Molecular y Genetica, Laboratorio de Genetica, Universidad de Extremadura, 06080 Badajoz, Spain
Francisco J. Carrillo
Affiliation:
Departamento de Bioquimica y Biologia Molecular y Genetica, Laboratorio de Genetica, Universidad de Extremadura, 06080 Badajoz, Spain
Alfonso Jimenez-Sanchez
Affiliation:
Departamento de Bioquimica y Biologia Molecular y Genetica, Laboratorio de Genetica, Universidad de Extremadura, 06080 Badajoz, Spain
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Summary

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Starvation for isoleucine inhibits chromosome, minichromosome and pBR322 DNA replication in a stringent strain of E. coli, but does not do so in a relaxed mutant. Starvation for other amino acids inhibits either chromosome and minichromosome replication in both strains. From these results we conclude that oriC and pBR322 replication are stringently regulated and that isoleucine seems not to be essential for the protein synthesis required at the initiation of oriC replication. Deprivation of isoleucine in a Rel− strain gives rise to amplification of minichromosome and pBR322 with a better yield of the latter plasmid than that following treatment with chloramphenicol.

Type
Research Article
Information
Genetics Research , Volume 51 , Issue 3 , June 1988 , pp. 173 - 177
Copyright
Copyright © Cambridge University Press 1988

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