Skip to main content
    • Aa
    • Aa

Effect of pre-treatment with 5-bromouracil in an ultraviolet sensitive strain of Neurospora crassa

  • Arnold Nemerofsky (a1)

5-Bromouracil was shown to enhance the mutation frequency in an ultraviolet sensitive strain of Neurospora crassa (uvs-2) that was previously irradiated with ultraviolet light. The design of the experiment eliminated light or dark repair as possible hypotheses to account for the observed enhancement. Since a similar effect was noted at the rib-1 locus in N. crassa, which is not a UV-sensitive strain, it appears that the error prone repair mechanism in N. crassa may be related to post-replication repair. This enhancement may be attributed to the interference of 5-bromouracil with post-replication repair. It is also postulated that the maximum increase in the mutation frequency occurs when the greatest number of unrepaired pre-mutational UV lesions are repaired in the presence of 5-bromouracil.

Hide All
Aoki S., Boyce R. P. & Howard-Flanders P. (1966). Sensitization of E. coli, to radiation by bromouracil. Nature 209, 686688.
Boyce R. P. & Howard-Flanders P. (1964). Release of UV light induced thymine dimers in E. coli. Proceedings of the National Academy of Sciences, U.S.A. 51, 293300.
Brockman H. E. & de Serres F. J. (1963). ‘Sorbose Toxicity’ in Neurospora. American Journal of Botany 50, 709714.
Catcheside D. G., (1974). Fungal genetics. Annual Review of Genetics 8, 279300.
de Serres F. J. (1971). Mutability of UV-sensitive strains of Neurospora crassa. Genetics 68, s14–s15.
Howard-Flanders P. & Boyce R. P. (1966). DNA repair and genetic recombination: studies on mutants of E. coli defective in these processes. Radiation Research (supplement 6), 156184.
Nemerofsky A. (1975). The interactive effect of UV irradiation and 5-BU at the rib-1 locus in N. crassa. Canadian Journal of Genetics and Cytology 17, 275278.
Schroeder A. L. (1974). Properties of a UV-sensitive Neurospora strain defective in pyrimidine dimer excision. Mutation Research 24, 916.
Setlow R. & Carrier W. (1964). The disappearance of thymine dimers from DNA, an error correcting mechanism. Proceedings of the National Academy of Sciences, U.S.A. 51, 226231.
Stadler D. & Smith D. D. (1968). A new mutation in Neurospora for sensitivity to U.V. light. Canadian Journal of Genetics and Cytology 10, 916919.
Tatum E. L., Barratt R. W., Fries N. & Bonner D. (1950). Biochemical strains of Neurospora produced by physical and chemical treatment. American Journal of Botany 37, 3846.
Westergaard M. & Mitchell H. K. (1947). Neurospora. V. A synthetic medium favouring sexual reproduction. American Journal of Botany 34, 573577.
Witkin E. M. (1966). Radiation induced mutation and their repair. Science 154, 13451347.
Witkin E. M. (1969 a). Ultraviolet induced mutation and DNA repair. Annual Review of Genetics 3, 525552.
Witkin E. M. (1969 b). The role of DNA repair in recombination and mutagenesis. Proceeding X11th International Congress of Genetics 3, 225245.
Worthy T. E. & Epler J. L. (1972). Repair of ultraviolet light-induced damage to the DNA of N. crassa. Journal of Bacteriology 110, 10101016.
Worthy T. E. & Epler J. L. (1973 a). Characterization of excision repair in N. crassa. Journal of Bacteriology 115, 498505.
Worthy T. E. & Epler J. L. (1973 b). Biochemical basis of radiation-sensitivity in mutants of Neurospora crassa. Mutation Research 19, 167173.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Genetics Research
  • ISSN: 0016-6723
  • EISSN: 1469-5073
  • URL: /core/journals/genetics-research
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Full text views

Total number of HTML views: 0
Total number of PDF views: 2 *
Loading metrics...

Abstract views

Total abstract views: 15 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 23rd October 2017. This data will be updated every 24 hours.