Hostname: page-component-7dd5485656-wjfd9 Total loading time: 0 Render date: 2025-10-27T07:58:07.508Z Has data issue: false hasContentIssue false
  • English
  • Français

Fine structure mapping and properties of mutations suppressing the lon mutation in Escherichia coli K-12 and B strains

Published online by Cambridge University Press:  14 April 2009

Ben F. Johnson
Ben F. Johnson
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Mutations, sulA and sulB, that suppress the UV sensitivity conferred by the lon mutation have been isolated and precisely positioned on the linkage map of Escherichia coli. The E. coli B strains Bs-3 and Bs-8 have been shown to possess sulA mutations. Also the E. coli K12 strain J6271 that possesses a suppressor of the lon mutation, previously designated as suf, has been shown to be a sulA mutation. A series of methylmethane sulphonate resistant derivatives of an E. coli K12 lon strain has been isolated and genetically characterized. In addition to sulA mutations, a second suppressor sulB was identified and located between leu and azi genes on the chromosome. Neither sulA or sulB mutations result in increased sensitivity to the antibiotics ampicillin, rifampicin, or actinomyein D, nor do they have any significant effect upon the overproduction of mucopolysaccharide caused by the lon mutation. Under some growth conditions the sulB mutation causes cells to be temperature sensitive for the cell division process at 42 °C.

Information

Type
Research Article
Information
Genetics Research , Volume 30 , Issue 3 , December 1977 , pp. 273 - 286
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Bachmann, B. J., Low, K. B. & Taylor, A. L. (1976). Recalibrated linkage map of Escherichia coli K-12. Bacteriological Reviews 40, 116–167.CrossRefGoogle ScholarPubMed
Berg, P. E., Gayda, R., Arni, H., Zehnbauer, B. & Markovitz, A. (1976). Cloning of Escherichia coli DNA that controls cell division and capsular polysaccharide synthesis. Proceedings of the National Academy of Sciences, U.S.A. 54, 1084–1091.Google Scholar
Burdett, I. D. J. & Murray, R. G. E. (1974). Septum formation in Escherichia coli: characterization of septal structure and the effects of antibiotics on cell division. Journal of Bacteriology 119, 303–324.CrossRefGoogle ScholarPubMed
Chung, Y. S., Greenberg, J. & Donch, J. (1974). Nonpermissiveness of strain B for Escherichia coli to P1kc; the isolation P1bc. Canadian Journal of Genetics and Cytology 16, 549–554.CrossRefGoogle Scholar
Dische, Z. & Shettles, L. B. (1948). A specific color reaction of methylpentoses and a spectrophotometric micromethod for their determination. Journal of Biological Chemistry 175, 595–603.CrossRefGoogle Scholar
Donch, J. & Greenberg, J. (1968). Genetic analysis of lon mutants of strain K-12 of Escherichia coli. Molecular and General Genetics 103, 105–115.CrossRefGoogle ScholarPubMed
Foulds, J. (1976). tolF locus in Escherichia coli: chromosomal location and relationship to loci cmlB and tolD. Journal of Bacteriology 128, 604–608.CrossRefGoogle ScholarPubMed
Freifelder, D. (1971). Genetic mapping by Hfr mating. In Methods in Enzymology, vol. 21, part D (ed. Colowick, S. P. and Kaplan, N. O.), pp. 438–444. New York: Academic Press.Google Scholar
Gayda, R. C., Yamamoto, L. T. & Markovitz, A. (1976). Second-site mutations in capR (lon) strains of Escherichia coli K-12 that prevent radiation sensitivity and allow bacteriophage lambda to lysogenize. Journal of Bacteriology 127, 1208–1216.CrossRefGoogle ScholarPubMed
George, J., Castellazzi, M. & Buttin, G. (1975). Prophage induction and cell division in E. coli. III. Mutations sfiA and sfiB restore division in tif and lon strains and permit the expression of mutator properties of tif. Molecular and General Genetics 140, 309–332.CrossRefGoogle Scholar
Green, M. H. L., Donch, J., Chung, Y. S. & Greenberg, J. (1969). Effect of inhibition of DNA synthesis on u.v. sensitive Bs strains of Escherichia coli. Genetical Research 14, 111–119.Google ScholarPubMed
Howard-Flanders, P., Simson, E. & Theriot, L. (1964). A locus that controls filament formation and sensitivity to radiation in Escherichia coli K12. Genetics 49, 237–246.CrossRefGoogle Scholar
James, R. & Gillies, N. E. (1973). The sensitivity of suppressed and unsuppressed lon strains of Escherichia coli to chemical agents which induce filamentation. Journal of General Microbiology 76, 429–436.CrossRefGoogle ScholarPubMed
Johnson, B. F. & Greenberg, J. (1975). Mapping of sul the suppressor of lon in Escherichia coli. Journal of Bacteriology 122, 570–574.CrossRefGoogle ScholarPubMed
Kantor, G. J. & Deering, R. A. (1968). Effect of nalidixic acid and hydroxyurea on division ability of Escherichia coli fil + and lon − strains. Journal of Bacteriology 95, 520–530.CrossRefGoogle ScholarPubMed
Nakamura, H. & Kawahara, M. (1974). Effects of amino acids on polysaccharide synthesis of Escherichia coli K-12 lon + and lon − strains. Botanical Magazine – Tokyo 87, 1–10.CrossRefGoogle Scholar
Normark, S. (1970). Genetics of a chain-forming mutant of Escherichia coli, transduction and dominance of the envA gene mediating increased penetration to some antibacterial agents. Genetical Research 16, 63–78.CrossRefGoogle ScholarPubMed
Organessian, M. G. & Organessian, H. G. (1973). A new type of suppressor of lon mutations of Escherichia coli. Genetics 74, 200.Google Scholar
Reeve, E. C. R. (1968). Genetic analysis of some mutations causing resistance to tetracycline in Escherichia coli K-12. Genetical Research 11, 303–309.CrossRefGoogle Scholar
Ricard, M. & Hirota, Y. (1973). Process of cellular division in Escherichia coli: physiological study on thermosensitive mutants defective in cell division. Journal of Bacteriology 116, 314–322.CrossRefGoogle Scholar
Roth, J. R. (1970). Genetic techniques in studies of bacterial metabolism. In Methods in Enzymology, vol. 27A (ed. Colowick, S. P. and Kaplan, N. O.), pp. 3–35. New York: Academic Press.Google Scholar
Walker, J. R., Kovarik, A., Allen, J. S. & Gustafson, R. A. (1975). Regulation of bacterial cell division: temperature-sensitive mutants of Escherichia coli that are defective in septum formation. Journal of Bacteriology 123, 693–703.CrossRefGoogle ScholarPubMed
Walker, J. R. & Pardee, A. B. (1968). Evidence for a relationship between deoxyribonucleic acid metabolism and septum formation in Escherichia coli. Journal of Bacteriology 95, 123–131.CrossRefGoogle ScholarPubMed
Wijsman, H. J. W. (1972). A genetic map of several mutations affecting the mucopeptide layer of Escherichia coli. Genetical Research 20, 65–74.CrossRefGoogle ScholarPubMed
Wijsman, H. J. W. & Koopman, C. R. M. (1976). The relation of the genes envA and ftsA in Escherichia coli. Molecular and General Genetics 147, 99–102.CrossRefGoogle ScholarPubMed
Witkin, E. M. (1946). Inherited differences in sensitivity to radiation in Escherichia coli. Proceedings of the National Academy of Sciences 32, 59–68.CrossRefGoogle ScholarPubMed
Wu, T. T. (1966). A model for three-point analysis of random general transduction. Genetics 54, 405–410.Google Scholar