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Genetic radiosensitivity of specific post-dictyate stages in mouse oöcytes

Published online by Cambridge University Press:  14 April 2009

R. G. Edwards  and
A. G. Searle
R. G. Edwards
Affiliation:
National Institute for Medical Research, Mill Hill, London, N.W.7
A. G. Searle
Affiliation:
M.R.C. Radiobiological Research Unit, Harwell, Berkshire, England
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The induction of dominant lethals after X-irradiation of dictyate, later meiotic stages, and the pronuclear stage after fertilization have been compared using the technique of induced ovulation in mice. The injection of gonadotrophins ensures that the time at which the synchronously dividing oöcytes reach any particular meiotic stage is accurately known. Embryonic lethality up to 13½ days was studied after 0 r., 100 r. and 200 r. acute X-irradiation. Metaphase I, anaphase I and metaphase II were the most sensitive stages, with LD50 's of about 120 r., 130 r. and 170 r. respectively. The dictyate and pronuclear stages were much less sensitive, with LD50's in the region of 500 r., and sensitivity rose steeply during prophase I. Numbers of corpora lutea decreased with irradiation, the decrease being greatest with irradiation at metaphase I and anaphase I. The ovulation of large numbers of eggs, which increased the preimplantation loss of embryos above normal, and the low luteal counts probably masked lethality to some extent. Results generally agree well with those reported in a number of plant and animal species.

Type
Research Article
Information
Genetics Research , Volume 4 , Issue 3 , November 1963 , pp. 389 - 398
Copyright
Copyright © Cambridge University Press 1963

References

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