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The induction of dominant lethal mutations in rats by alkane sulphonic esters

Published online by Cambridge University Press:  14 April 2009

M. Partington  and
H. Jackson
M. Partington
Affiliation:
Experimental Chemotherapy, Paterson Laboratories, Christie Hospital, Manchester 20
H. Jackson
Affiliation:
Experimental Chemotherapy, Paterson Laboratories, Christie Hospital, Manchester 20
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Sterility resulting from treatment of male rats with methyl ethane sulphonate and ethyl methane sulphonate was found to be due to the induction of dominant lethal mutations in spermatozoa and spermatids. Spermatids in the acrosome phase of development were found to be the most sensitive cells and at low doses of both compounds the majority of deaths occurred after implantation. The earlier cell stages, i.e. spermatogenia and spermatocytes, were the most sensitive to isopropyl methane sulphonate and Busulphan (Myleran). The action on spermatogonia was confirmed by spermatid counts and the time of occurrence of oligospermia or aspermia was calculated. Pre-implantation deaths occurred mainly in the early cleavage stages after both isopropyl methane sulphonate and Busulphan; at least part of these losses can be attributed to the induction of dominant lethal mutations in spermatocytes and early spermatids. Isopropyl methane sulphonate appears to be the more effective of the two drugs in this respect.

Type
Research Article
Information
Genetics Research , Volume 4 , Issue 3 , November 1963 , pp. 333 - 345
Copyright
Copyright © Cambridge University Press 1963

References

REFERENCES

Austin, C. R. & Smiles, J. (1948). Phase contrast microscopy in the study of fertilisation and early development of the rat eggs. J. R. micr. Soc. 68, 13–19.CrossRefGoogle Scholar
Bateman, A. J. (1958 a). Mutagenic sensitivity of maturing germ cells in the male mouse. Heredity, 12, 213–232.CrossRefGoogle Scholar
Bateman, A. J. (1958 b). The partition of dominant lethals in the mouse between unimplanted eggs and deciduomata. Heredity, 12, 467–475.CrossRefGoogle Scholar
Bateman, A. J. (1960). The induction of dominant lethals in rats and mice by triethylene-melamine. Genet. Res. 1, 381–392.CrossRefGoogle Scholar
Bock, M. & Jackson, H. (1957). The action of triethylenemelamine on the fertility of male rats. Brit. J. Pharmacol. 12, 1–7.Google ScholarPubMed
Brenneke, H. (1937). Strahlenschadigung von Mause- und Rattensperma beobachtetan der Frühentwicklung der Eier. Strahlentherapie, 60, 214–221.Google Scholar
Cattanach, B. M. (1959). The sensitivity of the mouse testis to the mutagenic action of TEM. Z. indukt. Abstamm-. u. VerebLehre, 90, 1–6.Google Scholar
Cattanach, B. M. & Edwards, R. G. (1958). The effects of triethylenemelamine on the fertility of male mice. Proc. roy. Soc. Edinb. B. 67, 54–64.Google Scholar
Clermont, Y., Leblond, C. P. & Messier, B. (1959). Durée du cycle de l'épithelium seminal du rat. Arch. Anat. micr. Morph. exp. 48, 37–56.Google Scholar
Edwards, R. G. (1957 a). The experimental induction of gynogenesis in the mouse. I. Irradiation of sperm by X-rays. Proc. roy. Soc. B, 146, 469–487.Google ScholarPubMed
Edwards, B. G. (1957 b). The experimental induction of gynogenesis in the mouse. II. Ultra-violet irradiation of the sperm. Proc. roy. Soc. B, 146, 488–504.Google ScholarPubMed
Jackson, H., Fox, B. W. & Craig, A. W. (1961). Antifertility substances and their assessment in the male rodent. J. Reprod. Fertil. 2, 447–465.CrossRefGoogle ScholarPubMed
Macmillan, E. W. & Harrison, R. G. (1955). The rate of passage of radio-opaque medium along the ductus epididymis of the rat. Studies on Fertility, I, 35–42.Google Scholar
Partington, M., Fox, B. W. & Jackson, H. (1963). Comparative action of some methane sulphonic esters on the cell populations of the rat testis. Exp. Cell. Res. (in press).Google Scholar