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Kinetics of F-curing by acridine orange in relation to the number of F-particles in Escherichia coli

Published online by Cambridge University Press:  14 April 2009

A. H. Stouthamer ,
P. G. de Haan  and
E. J. Bulten
A. H. Stouthamer
Affiliation:
Laboratory for Microbiology, State University, Utrecht, The Netherlands
P. G. de Haan
Affiliation:
Laboratory for Microbiology, State University, Utrecht, The Netherlands
E. J. Bulten
Affiliation:
Laboratory for Microbiology, State University, Utrecht, The Netherlands
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Acridine orange does not interfere with the distribution of the F-particles among the daughter cells but inhibits the multiplication of the episome.

In growing cells the generation time of the episome in the presence of acridine orange is longer than the generation time of the cell.

This lowers the number of episomes per cell to less than two copies at cell division.

F-particles rapidly multiply after transfer until a relatively small fixed number (3–4) is reached.

Type
Research Article
Information
Genetics Research , Volume 4 , Issue 2 , July 1963 , pp. 305 - 317
Copyright
Copyright © Cambridge University Press 1963

References

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