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A new method of detecting centromere linkage in homothallic yeast

Published online by Cambridge University Press:  14 April 2009

A. P. James
A. P. James
Affiliation:
Division of Biological Sciences, National Research Council of Canada, Ottawa, CanadaK1A 0R6
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Summary

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A method is described for detecting centromere linkage in Saccharomyces cerevisiae using a simple monohybrid for the locus in question. The method utilizes the two-spored asci produced by a normal homothallic diploid and depends upon a selective elimination of specific chromatids during spore formation. The pattern of selective elimination was not detected in a heterothallic strain but was found to be a dominant trait in an inter-strain cross. It was shown that gross chromosome aberrations are probably not responsible for the two-spored asci produced by either the heterothallic or homothallic strains.

Type
Research Article
Information
Genetics Research , Volume 23 , Issue 2 , April 1974 , pp. 201 - 206
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Hawthorne, D. C. (1955). The use of linear asci for chromosome mapping in Saccharomyces. Genetics 40, 511–518.CrossRefGoogle ScholarPubMed
Hawthorne, D. C. & Mortimer, R. K. (1960). Chromosome mapping in Saccharomyces: centromere-linked genes. Genetics 45, 1085–1110.CrossRefGoogle ScholarPubMed
James, A. P. (1973). Lethal sectoring in yeast. Genetics Supplement 73, 165–166.Google Scholar
Mortimer, R. K. & Hawthorne, D. C. (1966). Genetic mapping in Saccharomyces. Genetics 53, 165–173.CrossRefGoogle ScholarPubMed
Roman, H., Phillips, M. M. & Sands, S. M. (1955). Studies of polyploid Saccharomyces. I. Tetraploid segregation. Genetics 40, 546–561.CrossRefGoogle ScholarPubMed
Takahashi, T. (1962). Genetic segregation of two-ascus in Saccharomyces. Bulletin of Brewing Science 8, 1–9.Google Scholar