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Recombination and complementation between R factors in Escherichia coli K 12

Published online by Cambridge University Press:  14 April 2009

T. J. Foster  and
T. G. B. Howe
T. J. Foster
Affiliation:
Department of Bacteriology, The Medical School, University Walk, Bristol BS8 1TD
T. G. B. Howe
Affiliation:
Department of Bacteriology, The Medical School, University Walk, Bristol BS8 1TD
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Recombination between chloramphenicol-sensitive (Cms) mutants of Rl, and R100, has been demonstrated in Escherichia coli K12 rec+; it occurs at reduced frequency in recB and recC, and is not detectable in reeA, indicating that R factor recombination depends on host functions. Some mutants of R1 also recombine with an R100 mutant in a similar way. recA cells carrying an R1 and an R100 Cms mutant (hetero-R state) have a low level of chloramphenicol-resistance, and form a chloramphenicol acetyl transferase that has lower specific activity than enzyme from hosts carrying wild-type or recombinant factors. These results suggest the occurrence of interallelic complementation between mutant R factors.

Type
Research Article
Information
Genetics Research , Volume 18 , Issue 3 , December 1971 , pp. 287 - 297
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Barbour, S. D. & Clark, A. J. (1970). Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB + and recC + genes. Proceedings of the National Academy of Sciences, U.S.A. 65, 955–961.CrossRefGoogle ScholarPubMed
Egawa, R. & Hirota, Y. (1962). Inhibition of fertility by multiple drug-resistance factor (R) in Escherichia coli K-12. Japanese Journal of Genetics 37, 66–69.Google Scholar
Fincham, J. R. S. (1966). Genetic Complementation. New York and Amsterdam: Benjamin.Google Scholar
Fullbrook, P. D., Elson, S. W. & Slocombe, B. (1970). R-factor mediated β-lactamase in Pseudomonas aeruginosa. Nature, London 226, 1054–1056.CrossRefGoogle ScholarPubMed
Hashimoto, H. & Hirota, Y. (1966). Gene recombination and segregation of resistance factor R in Escherichia coli. Journal of Bacteriology 91, 51–62.CrossRefGoogle ScholarPubMed
Hashimoto, H. & Mitsuhashi, S. (1966). Drug resistance of enteric bacteria. VII. Recombination of R factors with tetracycline-sensitive mutants. Journal of Bacteriology 92, 1351–1356.CrossRefGoogle ScholarPubMed
Hashimoto, H., Iyobe, S. & Mitsuhashi, S. (1970). Transduction of R factors withphage P l from recombination-deficient Escherichia coli. Japanese Journal of Microbiology 14, 397–404.CrossRefGoogle Scholar
Hoar, D. I. (1970). Fertility regulation in F-like resistance transfer factors. Journal of Bacteriology 101, 916–920.CrossRefGoogle ScholarPubMed
Inouye, M. (1971). Pleiotropic effect of the recA gene of Escherichia coli: uncoupling of cell division from deoxyribonucleic acid replication. Journal of Bacteriology 106, 539–542.CrossRefGoogle ScholarPubMed
Lennox, E. S. (1955). Transduction of linked genetic characters of the host by bacteriophage P 1. Virology 1, 190–206.CrossRefGoogle Scholar
Low, B. (1968). Formation of merodiploids in matings with a class of Ree− recipient strains of Escherichia coli K12. Proceedings of the National Academy of Sciences, U.S.A. 60, 160–167.CrossRefGoogle Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Mise, K. & Suzuki, Y. (1968). Temperature-sensitive chloramphenicol acetyltransferase from Escherichia coli carrying mutant R factors. Journal of Bacteriology 95, 2124–2130.CrossRefGoogle ScholarPubMed
Mitsuhashi, S., Harada, K., Hashimoto, H., Kameda, M. & Suzuki, M. (1962). Combination of two types of transmissible drug-resistance factors in a host bacterium. Journal of Bacteriology 84, 9–16.CrossRefGoogle Scholar
Nisioka, T., Mitani, M. & Clowes, R. C. (1970). Molecular recombination between R-factor deoxyribonucleic acid molecules in Escherichia coli host cells. Journal of Bacteriology 103, 166–177.CrossRefGoogle ScholarPubMed
Shaw, W. V. (1966). Enzymatic chloramphenicol acetylation and R factor induced antibiotic resistance in Enterobacteriaceae. Antimicrobial Agents and Chemotherapy, 1966, pp. 221–226.Google Scholar
Shaw, W. V. (1967). The enzymatic acetylation of chloramphenicol by extracts of R factor-resistant Escherichia coli. Journal of Biological Chemistry 242, 687–693.CrossRefGoogle ScholarPubMed
Shaw, W. V., Bentley, D. W. & Sands, L. (1970). Mechanism of chloramphenicol resistance in Staphylococcus epidermidis. Journal of Bacteriology 104, 1095–1105.CrossRefGoogle ScholarPubMed
Shaw, W. V. & Brodsky, R. F. (1967). Chloramphenicol resistance by enzymatic acetylation: comparative aspects. Antimicrobial Agents and Chemotherapy, 1967, pp. 257–263.Google Scholar
Shaw, W. V. & Brodsky, R. F. (1968). Characterization of chloramphenicol acetyltransferase from chloramphenicol-resistant Staphylococcus aureus. Journal of Bacteriology 95, 28–36.CrossRefGoogle ScholarPubMed
Suzuki, Y. & Okamoto, S. (1967). The enzymatic acetylation of chloramphenicol by the multiple drug-resistant Escherichia coli carrying R factor. Journal of Biological Chemistry 242, 4722–4730.CrossRefGoogle ScholarPubMed
Takano, T. (1966). Behaviour of some episomal elements in a recombination-deficient mutant of Escherichia coli. Japanese Journal of Microbiology 10, 201–210.CrossRefGoogle Scholar
Watanabe, T. & Fukasawa, T. (1961). Episome-mediated transfer of drug resistance in Enterobacteriaceae. III. Transduction of resistance factors. Journal of Bacteriology 82, 202–209.CrossRefGoogle ScholarPubMed
Watanabe, T. & Lyang, K. W. (1962). Episome-mediated transfer of drug resistance in Enterobacteriaceae. V. Spontaneous segregation and recombination of resistance factors in Salmonella typhimurium. Journal of Bacteriology 84, 422–430.CrossRefGoogle ScholarPubMed
Watanabe, T., Nishida, H., Ogata, C., Arai, T. & Sato, S. (1964). Episome-mediated transfer of drug resistance in Enterobacteriaceae. VII. Two types of naturally occurring R factors. Journal of Bacteriology 88, 716–726.CrossRefGoogle ScholarPubMed
Willetts, N. S. & Clark, A. J. (1969). Characteristics of some multiply recombination-deficient strains of Escherichia coli. Journal of Bacteriology 100, 231–239.CrossRefGoogle ScholarPubMed
Yokota, T., Kanamaru, Y., Mori, R. & Akiba, T. (1969). Recombination between a thermo-sensitive kanamycin resistance factor and a nonthermosensitive multiple-drug resistance factor. Journal of Bacteriology 98, 863–873.CrossRefGoogle Scholar