Hostname: page-component-848d4c4894-cjp7w Total loading time: 0 Render date: 2024-06-20T03:20:47.088Z Has data issue: false hasContentIssue false
  • English
  • Français

Relative retention of mitochondrial markers in petite mutants: mitochondrially determined differences between RHO(+) strains

Published online by Cambridge University Press:  14 April 2009

P. L. Molloy ,
Anthony W. Linnane  and
H. B. Lukins
P. L. Molloy
Affiliation:
Department of Biochemistry, Monash University, Clayton, 3168, Australia
Anthony W. Linnane
Affiliation:
Department of Biochemistry, Monash University, Clayton, 3168, Australia
H. B. Lukins
Affiliation:
Department of Biochemistry, Monash University, Clayton, 3168, Australia

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The relative frequency of retention of two mitochondrial loci, determining resistance to oligomycin (oli1) and erythromycin (ery1), has been analysed in petite (rho(−)) mutants derived from a number of unrelated strains of Saccharomyces cerevisiae. The frequency of retention of one marker relative to the other in spontaneous petites showed marked variation dependent on the strain of origin. The differences between strains in this characteristic were shown to be mitochondrially determined. Further, for individual strains, the relative retention of the markers in petites derived after UV-irradiation varied considerably in several cases from that observed with spontaneous petites. The observations on relative marker retention and the varied effects of UV-irradiation are discussed in terms of possible structural differences in the mitochondrial genomes of the various strains.

Type
Research Article
Information
Genetics Research , Volume 26 , Issue 3 , December 1975 , pp. 319 - 325
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Avner, P. R., Coen, D., Dujon, B. & Slonimski, P. P. (1973). Mitochondrial genetics, IV. Allelism and mapping studies of oligomycin resistant mutants in S. cerevisiae. Molecular and General Genetics 125, 8–52.CrossRefGoogle ScholarPubMed
Bernardi, G., Prunell, A. & Kopecka, H. (1975). An analysis of the mitochondrial genome of yeast with restriction enzymes. In Proceedings of workshop on molecular biology of nucleocytoplasmic relationships during cell morphogenesis in some unicellular organisms. (In the Press.)Google Scholar
Bolotin, M., Coen, D., Deutsch, J., Dujon, B., Netter, P., Petrochilo, E. & Slonimski, P. P. (1971). La recombinaison des mitochondries chez Saccharomyces cerevisiae. Bulletin de l'Institut Pasteur 69, 213–219.Google Scholar
Clark-Walker, G. D. & Miklos, G. L. G. (1974). Mitochondrial genetics, circular DNA and the mechanism of the petite mutation in yeast. Genetical Research 24, 43–57.CrossRefGoogle ScholarPubMed
Howell, N., Trembath, M. K., Linnane, A. W. & Lukins, H. B. (1973). Biogenesis of mitochondria 30. An analysis of polarity of mitochondrial gene recombination and transmission. Molecular and General Genetics 122, 37–51.CrossRefGoogle ScholarPubMed
Linnane, A. W., Howell, N. & Lukins, H. B. (1974). Mitochondrial genetics. In The Biogenesis of Mitochondria: Transcriptional, Translational and Genetic Aspects (ed. Kroon, A. M. and Saccone, C.), pp. 193–213. New York: Academic Press.CrossRefGoogle Scholar
Molloy, P. L., Linnane, A. W. & Lukins, H. B. (1975). Biogenesis of mitochondria: analysis of deletion of mitochondrial antibiotic resistance markers in petite mutants of Saccharomyces cerevisiae. Journal of Bacteriology 122, 7–18.CrossRefGoogle ScholarPubMed
Nagley, P. & Linnane, A. W. (1970). Mitochondrial DNA deficient petite mutants of yeast. Biochemical and Biophysical Research Communications 39, 989–996.CrossRefGoogle ScholarPubMed
Prunell, A. & Bernardi, G. (1974). The mitochondrial genome of wild-type yeast cells. IV. Genes and spacers. Journal of Molecular Biology 86, 825–842.CrossRefGoogle ScholarPubMed
Raut, C. & Simpson, W. L. (1955). The effect of X-rays and ultraviolet light of different wavelengths on the production of cytochrome-deficient yeasts. Archives of Biochemistry and Biophysics 57, 218–228.CrossRefGoogle ScholarPubMed