Hostname: page-component-76fb5796d-skm99 Total loading time: 0 Render date: 2024-04-29T19:17:14.666Z Has data issue: false hasContentIssue false
  • English
  • Français

Structure, frequency and distribution of P elements in relation to P—M hybrid dysgenic male recombination in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

K. A. Exley  and
P. Eggleston
K. A. Exley
Affiliation:
Department of Genetics and Microbiology, University of Liverpool, P.O. Box 147, Liverpool L69 3BX
P. Eggleston
Affiliation:
Department of Genetics and Microbiology, University of Liverpool, P.O. Box 147, Liverpool L69 3BX
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The frequency and distribution of P elements were investigated in the third chromosomes of two wild-type strains of Drosophila melanogaster using in situ hybridization of biotinylated probes to the polytene chromosomes. The relationship between these data and the extent of hybrid dysgenesis was determined through assays of egg production, egg hatchability (F2 embryo lethality), snw destabilization and male recombination along the third chromosome. The results suggest that P-element distribution, frequency and structure are all contributory factors in the regulation of hybrid dysgenesis. Texas 6 was shown consistently to be a stronger P strain than Texas 1, eliciting greater reductions in fertility, more extensive snw destabilization and higher frequencies of male recombination. Clustering of male recombination events, arising from pre-meiotic crossing over, was evident among the dysgenic progeny of each strain. Male recombination and snw destabilization were independently distributed among the dysgenic males studied, suggesting that these traits represent separate P-mediated functions. The third chromosome male recombination maps produced by the two strains differed significantly from each other and from the published female meiotic and polytene chromosome maps. Male recombination breakpoints were associated with the original distribution of P sequences in the two strains and the results suggest that this relationship may be closer for potentially complete P factors than for P sequences in general. An analysis of sub-lines derived from individual recombinant males revealed that chromosomal breakpoints could also be associated with novel insertions following P-element transposition.

Type
Research Article
Information
Genetics Research , Volume 53 , Issue 3 , June 1989 , pp. 163 - 171
Copyright
Copyright © Cambridge University Press 1989

References

Bingham, P. M., Kidwell, M. G. & Rubin, G. M. (1982). The molecular basis of P–M hybrid dysgenesis: the role of the P element, a P strain specific transposon family. Cell 29, 995–1004.CrossRefGoogle Scholar
Birley, A. J., Couch, P. A. & Marson, A. (1981). Genetical variation for enzyme activity in a population of Drosophila melanogaster. VI. Molecular variation in the control of alcohol dehydrogenase (Adh) activity. Heredity 47, 185–196.CrossRefGoogle Scholar
Caligari, P. D. S. & Mather, K. (1980). Dominance, allele frequency and selection in a population of Drosophila melanogaster. Proceedings of the Royal Society of London B 208, 163–187.Google Scholar
Eggleston, P. & Kearsey, M. J. (1980). Hybrid dysgenesis in Drosophila: correlation between dysgenic traits. Heredity 44, 237–249.CrossRefGoogle ScholarPubMed
Eggleston, P. (1984). Hybrid dysgenesis in Drosophila melanogaster: the frequency and distribution of male recombination events. Heredity 52, 189–202.CrossRefGoogle Scholar
Engels, W. R. (1979). Extrachromosomal control of mutability in Drosophila melanogaster. Proceedings of the National Academy of Sciences, USA 76, 4011–4015.CrossRefGoogle ScholarPubMed
Engels, W. R. (1983). The P family of transposable elements in Drosophila. Annual Review of Genetics 17, 315–344.CrossRefGoogle Scholar
Engels, W. R. (1984). A trans-acting product needed for P-factor transposition in Drosophila. Science 226, 1194–1196.CrossRefGoogle ScholarPubMed
Engels, W. R., Benz, W. K., Preston, C. R., Graham, P. L., Phillis, R. W. & Robertson, H. M. (1987). Somatic effects of P element activity in Drosophila melanogaster: pupal lethality. Genetics 117, 745–757.CrossRefGoogle ScholarPubMed
Engels, W. R. & Preston, C. R. (1980). Components of hybrid dysgenesis in a wild population of Drosophila melanogaster. Genetics 95, 111–128.CrossRefGoogle Scholar
Engels, W. R. & Preston, C. R. (1981). Identifying P factors in Drosophila by means of chromosome breakage hot-spots. Cell 26, 421–428.CrossRefGoogle Scholar
Engels, W. R. & Preston, C. R. (1984). Formation of chromosome rearrangements by P factors in Drosophila. Genetics 107, 657–678.CrossRefGoogle ScholarPubMed
Engels, W. R., Preston, C. R., Thompson, P. & Eggleston, W. R. (1986). In situ hybridisation to Drosophila salivary chromosomes with biotinylated DNA probes and alkaline phosphatase. BRL Focus 8(1), 6–8.Google Scholar
Feinberg, A. P. & Vogelstein, B. (1983). A technique for radio-labelling DNA restriction and endonuclease fragments to high specific activity. Analytical Biochemistry 137, 266–267.Google Scholar
Gruenbaum, Y., Hochstrasser, M., Mathog, D., Saumweber, H., Agard, D. A. & Sedat, J. W. (1984). Spatial organisation of the Drosophila nucleus: a three dimensional cytogenetic study. In: Higher Order Structure in the Nucleus (ed. Cook, P. R. and Laskey, R. A.). Journal of Cell Science, Supplement 1, 223–234.Google Scholar
Hiraizumi, Y. (1979). A new method to distinguish between meiotic and premeiotic recombinational events in Drosophila melanogaster. Genetics 92, 543–554.CrossRefGoogle ScholarPubMed
Hsu, S. M., Raine, L. & Fanger, H. (1981). The use of avidin-biotin-peroxidase complex (ABC) in immuno-peroxidase techniques: a comparison between (ABC) and unlabelled antibody (PAP) procedures. Journal of Histochemistry and Cytochemistry 29, 577–581.CrossRefGoogle Scholar
Kelley, M. R., Kidd, S., Berg, R. L. & Young, M. W. (1987). Restriction of P element insertions at the notch locus of Drosophila melanogaster. Molecular and Cellular Biology 7, 1545–1548.Google ScholarPubMed
Kidwell, M. G. (1979). Hybrid dysgenesis in Drosophila melanogaster: the relationship between the P–M and I–R interaction systems. Genetical Research 33, 205–217.CrossRefGoogle Scholar
Kidwell, M. G., Kidwell, J. F. & Sved, J. A. (1977). Hybrid dysgenesis in Drosophila melanogaster: a syndrome of aberrant traits including mutation, sterility and male recombination. Genetics 86, 813–833.CrossRefGoogle ScholarPubMed
Kidwell, M. G. & Novy, J. B. (1979). Hybrid dysgenesis in Drosophila melanogaster: sterility resulting from gonadal dysgenesis in the P–M system. Genetics 92, 1127–1140.CrossRefGoogle ScholarPubMed
Kocur, G. J., Drier, E. A. & Simmons, M. J. (1986). Sterility and hypermutability in the P–M system of hybrid dysgenesis in Drosophila melanogaster. Genetics 114, 1147–1163.CrossRefGoogle Scholar
Lim, J. K. & Snyder, L. A. (1968). The mutagenic effects of two monofunctional alkylating chemicals on natural spermatozoa of Drosophila. Mutation Research 6, 127–137.CrossRefGoogle Scholar
Lindsley, D. L. & Grell, E. H. (1968). Genetic variations of Drosophila melanogaster. Carnegie Institution of Washington, Publication 627.Google Scholar
Linney, R., Barnes, B. W. & Kearsey, M. J. (1971). Variation for metrical characters in Drosophila populations. Heredity 27, 163–174.CrossRefGoogle ScholarPubMed
Manniatis, T., Fritch, E. F. & Sambrook, J. (1982). Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory.Google Scholar
O'Hare, K. & Rubin, G. M. (1983). Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell 34, 25–35.CrossRefGoogle ScholarPubMed
Pardue, M. L. & Gall, J. G. (1975). Nucleic acid hybridization to the DNA of cytological preparations. Methods in Cell Biology 10, 1–17.CrossRefGoogle Scholar
Rio, D. C., Laski, F. A. & Rubin, G. M. (1986). Identification and immunochemical analysis of biologically active Drosophila P element transposase. Cell 44, 21–32.CrossRefGoogle ScholarPubMed
Ronsseray, S. & Anxolabehere, D. (1986). Chromosomal distribution of P and I transposable elements in a natural population of Drosophila melanogaster. Chromosoma 94, 433–440.CrossRefGoogle Scholar
Rubin, G. M., Kidwell, M. G. & Bingham, P. M. (1982). The molecular basis of P–M hybrid dysgenesis: the nature of induced mutations. Cell 29, 987–994.CrossRefGoogle ScholarPubMed
Simmons, M. J., Raymond, J. D., Boedigheimer, M. J. & Zunt, J. R. (1987). The influence of non-autonomous P elements on hybrid dysgenesis in Drosophila melanogaster. Genetics 117, 671–685.CrossRefGoogle Scholar
Snedecor, G. W. & Cochran, W. G. (1980). Statistical Methods, 7th edidion, pp. 191–193. Iowa State University Press.Google Scholar
Sved, J. A. (1976). Hybrid dysgenesis in Drosophila melanogaster: a possible explanation in terms of spatial organisation of chromosomes. Australian Journal of Biological Sciences 29, 375–388.CrossRefGoogle ScholarPubMed