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The Use of a Selective Staphylococcal Broth v Direct Plating for the Recovery of Staphylococcus aureus

Published online by Cambridge University Press:  21 June 2016

R.L. Sautter ,
W.J. Brown  and
L.H. Mattman
R.L. Sautter*
Affiliation:
Department of Biological Sciences, Wayne State University, and the Department of Immunology/Microbiology and Pathology, Hutzel Hospital, Wayne State University, Detroit, Michigan
W.J. Brown
Affiliation:
Department of Biological Sciences, Wayne State University, and the Department of Immunology/Microbiology and Pathology, Hutzel Hospital, Wayne State University, Detroit, Michigan
L.H. Mattman
Affiliation:
Department of Biological Sciences, Wayne State University, and the Department of Immunology/Microbiology and Pathology, Hutzel Hospital, Wayne State University, Detroit, Michigan
*
Harrisburg Hospital, South Front Street, Harrisburg, PA 17101-2099
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Abstract

Nine hundred seventy-two cultures taken from the external nares and the vaginal vestibules of 54 women for the isolation of Staphylococcus aureus were studied. The swabs were plated directly to a trypticase soy agar plate containing 5% sheep blood and were then placed into a selective staphylococcal broth. Both culture methods were compared for the ability to recover S aureus.

Twenty percent (26/131) and 66% (38/58) of the S aureus-positive cultures taken from the nares and vagina respectively were cultured from the selective broth only. We believe that a selective staphylococcal broth should be used in addition to routine culture techniques to isolate S aureus from infection control surveillance cultures.

Information

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 9 , Issue 5 , May 1988 , pp. 204 - 205
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1988

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