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Assessment of Time to Clinical Response in Patients with Sepsis Treated Before and After Implementation of a Matrix-Assisted Laser Desorption Ionization Time-of-Flight Blood Culture Identification Algorithm

  • Joseph J. Carreno (a1), Ben M. Lomaestro (a2), Apryl L. Jacobs (a1), Rachel E. Meyer (a1), Ann Evans (a3) and Clemente I. Montero (a3)...
  • Please note a correction has been issued for this article.



To evaluate time to clinical response before and after implementation of rapid blood culture identification technologies.


Before-and-after trial.


Large, tertiary, urban, academic health-sciences center.


Patients >18 years old with sepsis and concurrent bacteremia or fungemia were included in the study; patients who were pregnant, had polymicrobial septicemia, or were transferred from an outside hospital were excluded.


Prior to the intervention, polymerase chain reaction was used to identify Staphylococcus species from positive blood cultures, and traditional laboratory techniques were used to identify non-staphylococcal species. After the intervention, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) assay and FilmArray were also used to identify additional species. During both periods, the antimicrobial stewardship team provided prospective audit and feedback for all patients on antibiotics.


A total of 219 patients were enrolled in the study: 115 patients prior to the intervention and 104 after the intervention. The median time to clinical response was statistically significantly shorter in the postintervention group than in the preintervention group (2 days vs 4 days, respectively; P=.002). By Cox regression, the implementation of MALDI-TOF and FilmArray was associated with shorter time to clinical response (hazard ratio [HR], 1.360; 95% confidence interval [CI], 1.018–1.816). After controlling for potential confounders, the study group was not independently associated with clinical response (adjusted HR, 1.279; 95% CI, 0.955–1.713). Mortality was numerically, but not statistically significantly, lower in the postintervention group than in the preintervention group (7.6% vs 11.4%; P=.342).


In the setting of an existing antimicrobial stewardship program, implementation of MALDI-TOF and FilmArray was associated with improved time to clinical response. Further research is needed to fully describe the effect of antimicrobial stewardship programs on time to clinical response.

Infect Control Hosp Epidemiol 2016;37:916–923


Corresponding author

Address correspondence to Joseph J. Carreno, PharmD, Albany College of Pharmacy and Health Sciences, School of Pharmacy and Pharmaceutical Sciences, Department of Pharmacy Practice, Albany, NY, 12208 (


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PREVIOUS PRESENTATION. These data were presented in part in Abstract K-659 at the 54th Interscience Conference on Antimicrobial Agents and Chemotherapy in Washington, District of Columbia, on September 7, 2014.



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Assessment of Time to Clinical Response in Patients with Sepsis Treated Before and After Implementation of a Matrix-Assisted Laser Desorption Ionization Time-of-Flight Blood Culture Identification Algorithm

  • Joseph J. Carreno (a1), Ben M. Lomaestro (a2), Apryl L. Jacobs (a1), Rachel E. Meyer (a1), Ann Evans (a3) and Clemente I. Montero (a3)...
  • Please note a correction has been issued for this article.


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