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Microbial Disruption Indices to Detect Colonization With Multidrug-Resistant Organisms

Published online by Cambridge University Press:  13 September 2017

Rafael Araos ,
Veronica Montgomery ,
Juan A. Ugalde ,
Graham M. Snyder  and
Erika M. C. D’Agata
Rafael Araos*
Affiliation:
Genomics and Resistant Microbes (GeRM) Group, Facultad de Medicina Clínica Alemana Universidad del Desarrollo, Santiago, Chile
Veronica Montgomery
Affiliation:
Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia
Juan A. Ugalde
Affiliation:
Centro de Genética y Genómica, Facultad de Medicina Clínica Alemana Universidad del Desarrollo, Santiago, Chile
Graham M. Snyder
Affiliation:
Department of Medicine, Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, MA
Erika M. C. D’Agata*
Affiliation:
Rhode Island Hospital, Brown University, Providence, Rhode Island
*
Address correspondence to Rafael Araos, MD, MMSc, Genomics and Resistant Microbes (GeRM) Group, Facultad de Medicina Clinica Alemana Universidad del Desarrollo, Avenida Vitacura 5951, Santiago, Chile (rafaaraos@gmail.com) or Erika M. C. D’Agata, Division of Infectious Diseases, Rhode Island Hospital, 593 Eddy Street, Aldrich 7, Providence, Rhode Island (edagata@lifespan.org).
Address correspondence to Rafael Araos, MD, MMSc, Genomics and Resistant Microbes (GeRM) Group, Facultad de Medicina Clinica Alemana Universidad del Desarrollo, Avenida Vitacura 5951, Santiago, Chile (rafaaraos@gmail.com) or Erika M. C. D’Agata, Division of Infectious Diseases, Rhode Island Hospital, 593 Eddy Street, Aldrich 7, Providence, Rhode Island (edagata@lifespan.org).
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Abstract

OBJECTIVE

To characterize the microbial disruption indices of hospitalized patients to predict colonization with multidrug-resistant organisms (MDROs).

DESIGN

A cross-sectional survey of the fecal microbiome was conducted in a tertiary referral, acute-care hospital in Boston, Massachusetts.

PARTICIPANTS

The study population consisted of adult patients hospitalized in general medical/surgical wards.

METHODS

Rectal swabs were obtained from patients within 48 hours of hospital admission and screened for MDRO colonization using conventional culture techniques. The V4 region of the 16S rRNA gene was sequenced to assess the fecal microbiome. Microbial diversity and composition, as well as the functional potential of the microbial communities present in fecal samples, were compared between patients with and without MDRO colonization.

RESULTS

A total of 44 patients were included in the study, of whom 11 (25%) were colonized with at least 1 MDRO. Reduced microbial diversity and high abundance of metabolic pathways associated with multidrug-resistance mechanisms characterized the fecal microbiome of patients colonized with MDRO at hospital admission.

CONCLUSIONS

Our data suggest that microbial disruption indices may be key to predicting MDRO colonization and could provide novel infection control approaches.

Infect Control Hosp Epidemiol 2017;38:1312–1318

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 38 , Issue 11 , November 2017 , pp. 1312 - 1318
Copyright
© 2017 by The Society for Healthcare Epidemiology of America. All rights reserved 

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Footnotes

PREVIOUS PRESENTATION: These data were presented in part as a poster at IDWeek 2016 on October 29, 2016, in New Orleans, Louisiana, poster #2229.

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