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Molecular Epidemiology of Nosocomial Infection: Analysis of Chromosomal Restriction Fragment Patterns by Pulsed-Field Gel Electrophoresis

Published online by Cambridge University Press:  21 June 2016

Neil L. Barg  and
Richard V. Goering
Neil L. Barg
Affiliation:
Creighton University School of Medicine, Omaha, Nebraska
Richard V. Goering*
Affiliation:
Creighton University School of Medicine, Omaha, Nebraska
*
Department of Medical Microbiology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178
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Extract

The epidemiological analysis of nosocomial pathogens is a subject of long-standing clinical interest. In recent years, molecular techniques have received increasing attention as a means of analyzing epidemiological interrelationships, thus leading to use of the term molecular epidemiology. Since chromosomal DNA represents a fundamental molecule of cellular identity, there has been particular interest in assessing chromosomal similarity as a measure of epidemiological relatedness.

One attractive approach has been to digest chromosomal DNA with restriction enzymes, resulting in a series of different sized fragments that form patterns when comparatively analyzed by agarose gel electrophoresis. In this context, differences in fragment patterns commonly are referred to as restriction-fragment length polymorphisms (RFLPs). Enzymes commonly used to cleave DNA typically recognize numerous sites within the bacterial chromosome. With such enzymes, restriction digestion of DNA from different bacterial isolates results in fragments that are too numerous to compare accurately after conventional agarose gel electrophoresis.

Type
Molecular Hospital Epidemiology
Information
Infection Control & Hospital Epidemiology , Volume 14 , Issue 10 , October 1993 , pp. 595 - 600
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1993 

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