Hostname: page-component-7f64f4797f-8cpv7 Total loading time: 0 Render date: 2025-11-03T13:36:22.665Z Has data issue: false hasContentIssue false
  • English
  • Français

Results of a local modified Delphi consensus on use of plasma metagenomic next-generation sequencing

Published online by Cambridge University Press:  28 October 2025

Caitlin Naureckas Li Open the ORCID record for Caitlin Naureckas Li [Opens in a new window] ,
Ravi Jhaveri Open the ORCID record for Ravi Jhaveri [Opens in a new window]  and
Sara Huston Open the ORCID record for Sara Huston [Opens in a new window]
Caitlin Naureckas Li*
Affiliation:
Division of Infectious Diseases, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL, USA Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA Center for Quality and Safety, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL, USA
Ravi Jhaveri
Affiliation:
Division of Infectious Diseases, Ann and Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL, USA Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA
Sara Huston
Affiliation:
Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA Mary Ann & J. Milburn Smith Child Health Outcomes, Research, and Evaluation Center, Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, IL, USA
*
Corresponding author: Caitlin Naureckas Li; Email: cli@luriechildrens.org
Get access Rights & Permissions [Opens in a new window]

Abstract

Molecular tests without well-defined test performance characteristics are increasingly available for diagnosis of infectious diseases. These tests present a diagnostic stewardship challenge for institutions. We share the results of a local modified Delphi consensus undertaken to define appropriate scenarios for use of plasma metagenomic next-generation sequencing.

Information

Type
Concise Communication
Information
Infection Control & Hospital Epidemiology , First View , pp. 1 - 3
Check for updates
Copyright
© The Author(s), 2025. Published by Cambridge University Press on behalf of The Society for Healthcare Epidemiology of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

Footnotes

Prior presentation: These data have not been previously shared in a preliminary report or abstract

References

Niles, DT, Lee, RA, Lamb, GS, Dhaheri, FA, Boguniewicz, J. Plasma cell-free metagenomic next generation sequencing in the clinical setting for the diagnosis of infectious diseases: A systematic review and meta-analysis. Diagn Microbiol Infect Dis 2023;105:115838.10.1016/j.diagmicrobio.2022.115838CrossRefGoogle ScholarPubMed
Blauwkamp, TA, Thair, S, Rosen, MJ, et al. Analytical and clinical validation of a microbial cell-free DNA sequencing test for infectious disease. Nat Microbiol 2019;4:663–674.10.1038/s41564-018-0349-6CrossRefGoogle ScholarPubMed
Naureckas Li, C, Blumenthal, JA, Sick-Samuels, AC. Use and Stewardship of molecular testing for diagnosis of infectious diseases: A cross-sectional survey. J Pediatr Infect Dis Soc 2025;14:piaf065. doi: 10.1093/jpids/piaf065 CrossRefGoogle ScholarPubMed
Naureckas Li, C, Jordan, N, Haymond, S, Koscinski, D, Jhaveri, R. Direct cost savings associated with reduction in plasma metagenomic sequencing. Infect Control Hosp Epidemiol. Published online October 21, 2025:1–3. doi: 10.1017/ice.2025.10329 CrossRefGoogle Scholar
FItch, K, Bernstein, SJ, Aguilar, MD, et al. The RAND/UCLA appropriateness method user’s Manual. https://www.rand.org/content/dam/rand/pubs/monograph_reports/2011/MR1269.pdf. Published online 2001. Accessed August 8, 2025.Google Scholar
Mukherjee, N, Hugé, J, Sutherland, WJ, et al. The Delphi technique in ecology and biological conservation: applications and guidelines. Methods Ecol Evol 2015;6:1097–1109.10.1111/2041-210X.12387CrossRefGoogle Scholar
Shean, RC, Garrett, E, Malleis, J, Lieberman, JA, Bradley, BT. A retrospective observational study of mNGS test utilization to examine the role of diagnostic stewardship at two academic medical centers. J Clin Microbiol 2024;62:e0060524.10.1128/jcm.00605-24CrossRefGoogle ScholarPubMed
Goggin, KP, Fox, TG, Jaggi, P, Shane, AL, Gonzalez, MD. Impact of a new diagnostic stewardship team on utilization and interpretation of a microbial cell-Free DNA detection test. J Pediatr Infect Dis Soc 2023;12 (Supplement_1):S24.10.1093/jpids/piad070.049CrossRefGoogle Scholar
Fowler, VG Jr, Durack, DT, Selton-Suty, C, et al. The 2023 duke-international society for cardiovascular infectious diseases criteria for infective endocarditis: Updating the modified duke criteria. Clin Infect Dis 2023;77:518–526.10.1093/cid/ciad271CrossRefGoogle ScholarPubMed
Bergin, SP, Chemaly, RF, Dadwal, SS, et al. Plasma microbial cell-free DNA sequencing in immunocompromised patients with pneumonia: A prospective observational study. Clin Infect Dis Off Publ Infect Dis Soc Am 2024;78:775–784.10.1093/cid/ciad599CrossRefGoogle ScholarPubMed
Supplementary material: File

Naureckas Li et al. supplementary material

Naureckas Li et al. supplementary material
Download Naureckas Li et al. supplementary material(File)
File 418.6 KB