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Transmission of Staphylococcus aureus from dry surface biofilm (DSB) via different types of gloves

Published online by Cambridge University Press:  13 November 2018

Shamaila Tahir Open the ORCID record for Shamaila Tahir [Opens in a new window] ,
Durdana Chowdhury ,
Mark Legge ,
Honghua Hu ,
Greg Whiteley ,
Trevor Glasbey ,
Anand K. Deva  and
Karen Vickery
Shamaila Tahir*
Affiliation:
Surgical Infection Research Group, Faculty of Medicine and Health Sciences (FMHS), Macquarie University, Sydney, New South Wales, Australia
Durdana Chowdhury
Affiliation:
Surgical Infection Research Group, Faculty of Medicine and Health Sciences (FMHS), Macquarie University, Sydney, New South Wales, Australia
Mark Legge
Affiliation:
Surgical Infection Research Group, Faculty of Medicine and Health Sciences (FMHS), Macquarie University, Sydney, New South Wales, Australia
Honghua Hu
Affiliation:
Surgical Infection Research Group, Faculty of Medicine and Health Sciences (FMHS), Macquarie University, Sydney, New South Wales, Australia
Greg Whiteley
Affiliation:
Whiteley Corporation, Tomago, Newcastle, New South Wales, Australia
Trevor Glasbey
Affiliation:
Whiteley Corporation, Tomago, Newcastle, New South Wales, Australia
Anand K. Deva
Affiliation:
Surgical Infection Research Group, Faculty of Medicine and Health Sciences (FMHS), Macquarie University, Sydney, New South Wales, Australia
Karen Vickery
Affiliation:
Surgical Infection Research Group, Faculty of Medicine and Health Sciences (FMHS), Macquarie University, Sydney, New South Wales, Australia
*
Author for correspondence: Shamaila Tahir, Level 1, 75 Talavera Road, Macquarie University, NSW, Australia. E-mail: shamaila.tahir@mq.edu.au
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Abstract

Background

Pathogens can survive for extended periods when incorporated into biofilm on dry hospital surfaces (ie, dry-surface biofilm, DSB). Bacteria within biofilm are protected from desiccation and have increased tolerance to cleaning agents and disinfectants.

Objective

We hypothesized that gloved hands of healthcare personnel (HCP) become contaminated with DSB bacteria and hence may transmit bacteria associated with healthcare-associated infections (HAIs).

Method

Staphylococcus aureus DSB was grown in vitro on coupons in a bioreactor over 12 days with periodic nutrition interspersed with long periods of dehydration. Each coupon had ~107 DSB bacterial cells. Transmission was tested with nitrile, latex, and surgical gloves by gripping DSB-covered coupons then pressing finger tips onto a sterile horse blood agar surface for up to 19 consecutive touches and counting the number of colony-forming units (CFU) transferred. Coupons were immersed in 5% neutral detergent to simulate cleaning, and the experiment was repeated.

Results

Bacterial cells were readily transmitted by all 3 types of gloves commonly used by HCP. Surprisingly, sufficient S. aureus to cause infection were transferred from 1 DSB touch up to 19 consecutive touches. Also, 6 times more bacteria were transferred by nitrile and surgical gloves than to latex gloves (P <.001). Treating the DSB with 5% neutral detergent increased the transmission rate of DSB bacteria 10-fold.

Conclusion

Staphylococcus aureus incorporated into environmental DSB and covered by extracellular polymeric substances readily contaminates gloved hands and can be transferred to another surface. These results confirm the possibility that DSB contributes to HAI acquisition.

Type
Original Article
Information
Infection Control & Hospital Epidemiology , Volume 40 , Issue 1 , January 2019 , pp. 60 - 64
Copyright
© 2018 by The Society for Healthcare Epidemiology of America. All rights reserved. 

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Footnotes

Cite this article: Tahir S, et al. (2019). Transmission of Staphylococcus aureus from dry surface biofilm (DSB) via different types of gloves. Infection Control & Hospital Epidemiology 2019, 40, 60–64. doi: 10.1017/ice.2018.285

References

1. Al-Tawfiq, JA, Tambyah, PA. Healthcare-associated infections (HAI) perspectives. J Infect Public Health 2014;7:339–344.Google Scholar
2. Khan, HA, Baig, FK, Mehboob, R. Nosocomial infections: Epidemiology, prevention, control and surveillance. Asia Pac J Trop Biomed 2017;7:478–482.Google Scholar
3. WHO. Health Care-Associated Infections Fact Sheet. World Health Organization; 2014.Google Scholar
4. WHO. Guidelines on Core Components of Infection Prevention and Control Programs at the National and Acute Health Care Facility Level. World Health Organization; 2016.Google Scholar
5. Dancer, SJ. The role of environmental cleaning in the control of hospital-acquired infection. J. Hosp. Infect. 2009;73:378–385.Google Scholar
6. Claro, T, Galvin, S, Cahill, O, Fitzgerald-Hughes, D, Daniels, S, Humphreys, H. What is the best method? Recovery of methicillin-resistant Staphylococcus aureus and extended-spectrum β-lactamase–producing Escherichia coli from inanimate hospital surfaces. Infect Control Hosp Epidemiol 2014;35:869–871.Google Scholar
7. Otter, JA, Yezli, S, Salkeld, JA, French, GL. Evidence that contaminated surfaces contribute to the transmission of hospital pathogens and an overview of strategies to address contaminated surfaces in hospital settings. Am J Infect Control 2013;41:S6–S11.Google Scholar
8. Kramer, A, Schwebke, I, Kampf, G. How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infect Dis 2006;6:130.Google Scholar
9. Hu, H, Johani, K, Gosbell, IB, et al. Intensive care unit environmental surfaces are contaminated by multidrug-resistant bacteria in biofilms: combined results of conventional culture, pyrosequencing, scanning electron microscopy, and confocal laser microscopy. J Hosp Infect 2015;91:35–44.Google Scholar
10. Nseir, S, Blazejewski, C, Lubret, R, Wallet, F, Courcol, R, Durocher, A Risk of acquiring multidrug‐resistant gram‐negative bacilli from prior room occupants in the intensive care unit. Clin Microbiol Infect 2011;17:1201–1208.Google Scholar
11. Shaughnessy, MK, Micielli, RL, DePestel, DD, et al. Evaluation of hospital room assignment and acquisition of Clostridium difficile infection. Infect Control Hosp Epidemiol 2011;32:201–206.Google Scholar
12. Boyce, JM. Environmental contamination makes an important contribution to hospital infection. J Hosp Infect 2007;65 Suppl 2:50–54.Google Scholar
13. Bhalla, A, Pultz, NJ, Gries, DM, et al. Acquisition of nosocomial pathogens on hands after contact with environmental surfaces near hospitalized patients. Infect Control Hosp Epidemiol 2004;25:164–167.Google Scholar
14. Hygiene, H. Hand hygiene Australia. http://www.hha.org.au/home.aspx. Published 2017. Accessed August 15, 2017.Google Scholar
15. Kwok, YLA, Juergens, CP, McLaws, M-L Automated hand hygiene auditing with and without an intervention. Am J Infect Control 2016;44:1475–1480.Google Scholar
16. Chowdhary, D, Tahir, S, Legge, M, et al. Transfer of dry surface biofilm in healthcare environment: the role of healthcare worker’s hands as vehicles. J Hosp Infect 2018. doi: 10.1016/j.jhin.2018.06.021.Google Scholar
17. WHO. Practical Guidelines for Prevention of Hospital-Acquired Infections. Geneva: World Health Organization; 2002.Google Scholar
18. NHMRC. Australian Guidelines for the Prevention and Control of Infection in Healthcare. Commonwealth of Australia; 2010.Google Scholar
19. Girou, E, SHT, Chai, Oppein, F, et al. Misuse of gloves: the foundation for poor compliance with hand hygiene and potential for microbial transmission? J Hosp Infect. 2004;57:162–169.Google Scholar
20. Wilson, J, Loveday, H. Does glove use increase the risk of infection? Nurs Times 2014;110:12–15 Google Scholar
21. Girou, E, Chai, SH, Oppein, F, et al. Misuse of gloves: the foundation for poor compliance with hand hygiene and potential for microbial transmission? J Hosp Infect 2004;57:162–169.Google Scholar
22. Moore, G, Dunnill, CW, Wilson, AP. The effect of glove material upon the transfer of methicillin-resistant Staphylococcus aureus to and from a gloved hand. Am J Infect Control 2013;41:19–23.Google Scholar
23. Almatroudi, A, Hu, H, Deva, A, et al. A new dry-surface biofilm model: An essential tool for efficacy testing of hospital surface decontamination procedures. J Microbiol Methods 2015;117:171–176.Google Scholar
24. Johani, K, Abualsaud, D, Costa, DM, et al. Characterization of microbial community composition, antimicrobial resistance and biofilm on intensive care surfaces. J Infect Pub Health 2018;11:418–424.Google Scholar
25. Galvin, S, Dolan, A, Cahill, O, Daniels, S, Humphreys, H Microbial monitoring of the hospital environment: Why and how? J Hosp Infect 2012;82:143–151.Google Scholar
26. Pérez-Rodríguez, F, Valero, A, Carrasco, E, García, RM, Zurera, G Understanding and modelling bacterial transfer to foods: a review. Trend Food Sci Tech 2008;19:131–144.Google Scholar
27. Maki, DG, Alvarado, CJ, Hassemer, CA, Zilz, MA Relation of the inanimate hospital environment to endemic nosocomial infection. N Engl J Med 1982;307:1562–1566.Google Scholar
28. Mallison, GF, Haley, RW Microbiologic sampling of the inanimate environment in US hospitals, 1976–1977. Am J Med 1981;70:941–946.Google Scholar
29. Sehulster, L, Chinn, RYW. Guidelines for environmental infection control in healthcare facilities: recommendations of CDC and the Healthcare Infection Control Practices Advisory Committee (HICPAC). Morbid Mortal Wkly Rept 2003;52:1–43.Google Scholar
30. Russotto, V, Cortegiani, A, Raineri, SM, Giarratano, A Bacterial contamination of inanimate surfaces and equipment in the intensive care unit. J Intens Care 2015;3:54.Google Scholar
31. Otter, JA, Vickery, K, Walker, JT, et al. Surface-attached cells, biofilms and biocide susceptibility: implications for hospital cleaning and disinfection. J Hosp Infect 2015;89:16–27.Google Scholar
32. Almatroudi, A, Gosbell, IB, Hu, H, et al. Staphylococcus aureus dry-surface biofilms are not killed by sodium hypochlorite: implications for infection control. J Hosp Infect 2016;93:263–270.Google Scholar
33. Randle, J, Arthur, A, Vaughan, N Twenty-four-hour observational study of hospital hand hygiene compliance. J Hosp Infect 2010;76:252–255.Google Scholar
34. Smiddy, MP, O’Connell, R, Creedon, SA. Systematic qualitative literature review of health care workers’ compliance with hand hygiene guidelines. Am J Infect Control 2015;43:269–274.Google Scholar
35. Luangasanatip, N, Hongsuwan, M, Limmathurotsakul, D, et al. Comparative efficacy of interventions to promote hand hygiene in hospital: systematic review and network metaanalysis. BMJ 2015;351:h3728.Google Scholar
36. Huslage, K, Rutala, WA, Gergen, MF, Sickbert-Bennett, EE, Weber, DJ. Microbial assessment of high-, medium-, and low-touch hospital room surfaces. Infect Control Hosp Epidemiol 2013;34:211–212.Google Scholar
37. Almatroudi, A Patients’ Safety and Hospital Acquired Infections: The Size of the Problem of Biofilms on the Health Care Surfaces. Sydney, Australia: Department of Biomedical Sciences, Macquarie University; 2016.Google Scholar
38. Hayden, MK, Blom, DW, Lyle, EA, Moore, CG, Weinstein, RA. Risk of hand or glove contamination after contact with patients colonized with vancomycin-resistant enterococcus or the colonized patients’ environment. Infect Control Hosp Epidemiol 2008;29:149–154.Google Scholar
39. Foster, WD, MSR, Hutt. Experimental staphylococcal infections in man. Lancet 1960;276:1373–1376.Google Scholar
40. PBJ, Vermeltfoort, van der Mei, HC, Busscher, HJ, JMM, Hooymans, Bruinsma, GM. Physicochemical factors influencing bacterial transfer from contact lenses to surfaces with different roughness and wettability. J Biomed Mater Res B: Appl Biomat 2004;71B:336–342.Google Scholar
41. Knobben, BAS, van der Mei, HC, van Horn, JR, Busscher, HJ Transfer of bacteria between biomaterials surfaces in the operating room—an experimental study. J Biomed Mater Res A 2007;80A:790–799.Google Scholar
42. Rogusz, A, Stewart, M, Hunter, J, et al. How quickly do hospital surfaces become contaminated after detergent cleaning? Healthcare Infect 2013;18:3–9.Google Scholar