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Identification of root-knot nematode species occurring on tomatoes in Kenya: use of isozyme phenotypes and PCR-RFLP

Published online by Cambridge University Press:  13 June 2012

Rael Birithia ,
Wanjohi Waceke ,
Peter Lomo  and
Daniel Masiga
Rael Birithia*
Affiliation:
Molecular Biology and Biotechnology Department, International Centre of Insect Physiology and Ecology (icipe –African Insect Science for Food and Health), PO Box 30772-00100, Nairobi, Kenya Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology, PO Box 62000-00200, Nairobi, Kenya
Wanjohi Waceke
Affiliation:
Department of Agricultural Science and Technology, Kenyatta University, PO Box 43844-00100, Nairobi, Kenya
Peter Lomo
Affiliation:
Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology, PO Box 62000-00200, Nairobi, Kenya
Daniel Masiga
Affiliation:
Molecular Biology and Biotechnology Department, International Centre of Insect Physiology and Ecology (icipe –African Insect Science for Food and Health), PO Box 30772-00100, Nairobi, Kenya
*
*E-mail: rbirithia@icipe.org
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Abstract

Root-knot nematodes (RKN) are serious pests of tomato production in Kenya. Accurate identification of the plant parasitic nematodes is important for their effective management. A study conducted to assess their prevalence and identify RKN occurring on tomatoes in Central Kenya showed infestation of the crop in all the three districts. Of the total sampled plants (N = 900), the RKN infestation level ranged from 28 to 62%. RKN disease severity ranged from 2.5 to 5.3 in all the locations. Meloidogyne incognita,M. javanica and M. arenaria were the only species found infesting tomatoes in these areas. Female nematodes sampled from the symptomatic root system that had root galls characteristic of RKN were analysed by isozyme phenotypes of esterase (EST) and malate dehydrogenase (MDH). EST phenotype was polymorphic and enabled identification of the three different species, while MDH was monomorphic. Polymerase chain reaction-restriction fragment length polymorphism with region between mitochondrial cytochrome oxidase subunit II and large subunit ribosomal RNA (mtDNA COII-LSUrRNA) using HinfI showed that all isolates of M. incognita could be digested into three restriction fragments of about 1300, 400 and 100 bp, except for one species that showed an additional restriction site, giving four fragments of 900, 420, 380 and 100 bp. The 1800 bp PCR product of M. javanica was not digested by HinfI. Meloidogyne arenaria (EST phenotype A1) PCR product was digested into two restriction fragments of about 1700 and 100 bp, while M. arenaria (EST phenotype A2) had two restriction fragments at 1100 and 100 bp.

Keywords

Meloidogyne spptomatoesisozyme profilesmolecular identification
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 32 , Issue 2 , June 2012 , pp. 78 - 84
Copyright
Copyright © ICIPE 2012

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