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Population structure of three invasive congeneric teasel (Dipsacus) species

Published online by Cambridge University Press:  29 February 2024

John F. Gaskin*
Natalie West
Brian G. Rector
Corresponding author: John F. Gaskin; Email:


Three species of the Old World genus Dipsacus L. are considered invasive in the Americas, yet they may differ in how they spread and reproduce and in their genetic diversity. Differences in invasion method may suggest that different management techniques are needed for each species. We performed genetic analyses on 572 plants in 69 populations from the United States, Argentina, and Eurasia with the goals of analyzing taxonomy, diversity, mode of reproduction, population structure, and founder effect of each of these species’ invasions, as well as looking for evidence of recent or ongoing hybridization. We found Indian teasel [Dipsacus sativus (L.) Honck.] to be lowest in diversity and possibly reliant on self-pollination more than the other species, Fuller’s teasel (Dipsacus fullonum L.) and cutleaf teasel (Dipsacus laciniatus L.). We found no evidence of hybridization within the invasions and no support for D. sativus as a subspecies of D. fullonum. The closest genetic matches of D. fullonum from the United States to the native range were with Hungary and Spain, while the closest match for D. fullonum between Argentina and the native range was with Spain. Dipsacus laciniatus from the United States most closely matched with samples from Russia. Population structure information regarding these three weedy Dipsacus species can help us understand their invasive processes as well as give insight into their management and the development of a biological control program.

Research Article
Creative Commons
This is a work of the US Government and is not subject to copyright protection within the United States. Published by Cambridge University Press on behalf of Weed Science Society of America.
© USDA Agricultural Research Service, 2024

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Associate Editor: Marie Jasieniuk, University of California, Davis


Bentivegna, DJ (2006) Biology and Management of Cut-leaved Teasel (Dipsacus laciniatus L.) in Central Missouri. Pages 31–37. Ph.D dissertation. Columbia: University of Missouri–ColumbiaGoogle Scholar
Bentivegna, DJ, Smeda, RJ (2011a) Cutleaf teasel (Dipsacus laciniatus): seed development and persistence. Invasive Plant Sci Manag 4:3137 10.1614/IPSM-D-10-00026.1CrossRefGoogle Scholar
Bentivegna, DJ, Smeda, RJ (2011b) Seed production of cut-leaf teasel (Dipsacus laciniatus) in central Missouri. Biologia 66:807812 CrossRefGoogle Scholar
Bentivegna, DJ, Smeda, RJ (2012) Integrated management of cutleaf teasel (Dipsacus laciniatus) along roadsides in Missouri, USA. Int J Pest Manag 58:147152 CrossRefGoogle Scholar
Byers, JE, Reichard, S, Randall, JM, Parker, IM, Smith, CS, Lonsdale, WM, Atkinson, IAE, Seastedt, TR, Williamson, M, Chornesky, E, Hayes, D (2002) Directing research to reduce the impacts of nonindigenous species. Conserv Biol 16:630640 10.1046/j.1523-1739.2002.01057.xCrossRefGoogle Scholar
Cristofaro, M, Roselli, G, Marini, F, de Lillo, E, Petanovic, RU, Vidovic, B, Augé, M, Rector, BG (2020) Open field evaluation of Aculdoes altamurgiensis, a recently described eriophyid species associated with medusahead (Taeniatherum caput-medusae). Biocontrol Sci Technol 30:339350 CrossRefGoogle Scholar
Daddario, JFF, Tucat, G, Fernandez, OA, Bentivegna, DJ (2021) Efficacy of increasing application rates and combination of herbicides and mowing at different growth stages of common teasel (Dipsacus fullonum). Weed Technol 35:476484 10.1017/wet.2020.122CrossRefGoogle Scholar
Dallimore, W (1912) The Fuller’s teasel (Dipsacus fullonum L.). Bull. Misc. Inform. Kew 1912(7):345350 Google Scholar
Dlugosch, KM, Parker, IM (2008) Founding events in species invasions: genetic variation, adaptive evolution, and the role of multiple introductions. Mol Ecol 17:431449 CrossRefGoogle ScholarPubMed
Donaldson, S, Rafferty, D (2002) Identification and Management of Common Teasel (Dipsacus fullonum). Fact Sheet-02-40. Reno: University of Nevada, Cooperative Extension. 2 pGoogle Scholar
Dudley, MP, Parrish, JAD, Post, L, Helm, CG, Wiedenmann, RN (2009) The effects of fertilization and time of cutting on regeneration and seed production of Dipsacus laciniatus . Nat Areas J 29:140145 CrossRefGoogle Scholar
Earl, DA, vonHoldt, BM (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Gen Res 4:359361 CrossRefGoogle Scholar
Evanno, G, Regnaut, S, Goudet, J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:26112620 CrossRefGoogle ScholarPubMed
Falush, D, Stephens, M, Pritchard, JK (2003) Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164:15671587 CrossRefGoogle ScholarPubMed
Falush, D, Stephens, M, Pritchard, JK (2007) Inference of population structure using multilocus genotype data: dominant markers and null alleles. Mol Ecol Notes 7:574578 10.1111/j.1471-8286.2007.01758.xCrossRefGoogle ScholarPubMed
Ferguson, IK, Brizicky, GK (1965) Nomenclatural notes on Dipsacus fullonum and Dipsacus sativus . J Arnold Arbor Harv Univ 46:362365 CrossRefGoogle Scholar
Gaskin, JF, Bon, MC, Cock, MJ, Cristofaro, M, De Biase, A, De Clerck-Floate, R, Ellison, CA, Hinz, HL, Hufbauer, RA, Julien, MH, Sforza, R (2011) Applying molecular-based approaches to classical biological control of weeds. Biol Control 58:121 CrossRefGoogle Scholar
Gaskin, JF, Coombs, E, Kelch, DG, Kei, DJ, Porter, M, Susanna, A (2020) Carduus cinereus (Asteraceae)—new to North America. Madroño 66:142147 10.3120/0024-9637-66.4.142CrossRefGoogle Scholar
Gaskin, JF, Kazmer, DJ (2009) Introgression between invasive saltcedars (Tamarix chinensis and T. ramosissima) in the USA. Biol Invasions 11:11211130 10.1007/s10530-008-9384-1CrossRefGoogle Scholar
Gaskin, JF, Schwarzländer, M, Kinter, CL, Smith, JF, Novak, SJ (2013) Propagule pressure, genetic structure, and geographic origins of Chondrilla juncea (Asteraceae): an apomictic invader on three continents. Am J Bot 100:18711882 CrossRefGoogle ScholarPubMed
Gerlach, JD Jr, Rice, KJ (2003) Testing life history correlates of invasiveness using congeneric plant species. Ecol Appl 13:167179 CrossRefGoogle Scholar
Giolitti, F, Bejerman, N, Lenardon, S (2009) Dipsacus fullonum: an alternative host of sunflower chlorotic mottle virus in Argentina. J Phytopathol 157:325328 CrossRefGoogle Scholar
Gleason, HA, Cronquist, A (1991) Manual of Vascular Plants of Northeastern United States and Adjacent Canada. 2nd ed. New York: New York Botanical Garden. 910 pCrossRefGoogle Scholar
Grosholz, E (2010) Avoidance by grazers facilitates spread of an invasive hybrid plant. Ecol Lett 13:145153 CrossRefGoogle ScholarPubMed
Gross, BL, Rieseberg, LH (2005) The ecological genetics of homoploid hybrid speciation. Heredity 96:241252 CrossRefGoogle ScholarPubMed
Gross, KL (1984) Effects of seed size and growth form on seedling establishment of six monocarpic perennial plants. J Ecol 72:369387 CrossRefGoogle Scholar
Gucker, CL (2009) Dipsacus fullonum, D. laciniatus. In: Fire Effects Information System, [Online]. U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station, Fire Sciences Laboratory (Producer). Accessed March 19, 2024Google Scholar
Hao, JH, Lv, SS, Bhattacharya, S, Fu, JG (2017) Germination response of four alien congeneric Amaranthus species to environmental factors. PLoS ONE 12:e0170297 CrossRefGoogle ScholarPubMed
Hillis, DM, Moritz, C, Mable, BK (1996) Molecular Systematics. 2nd ed. Sunderland, MA: Sinauer. 655 p Google Scholar
Hilty, J (2009) Cut-leaved Teasel (Dipsacus laciniatus): Teasel Family (Dipsacaceae). Accessed: November 16, 2023Google Scholar
Wildflowers, Illinois (2023) Teasel (Dipsacus fullonum): Teasel Family (Dipsacaceae). Accessed: November 16, 2023Google Scholar
iNaturalist (n.d.) Home page. Accessed: November 16, 2023Google Scholar
Jepson Flora Project, eds (2023) Jepson eFlora. Jepson Herbarium. Accessed: November 16, 2023Google Scholar
Kumar, S, Stecher, G, Li, M, Knyaz, C, Tamura, K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:15471549 CrossRefGoogle ScholarPubMed
Lake, TA, Briscoe Runquist, RD, Moeller, DA (2020) Predicting range expansion of invasive species: Pitfalls and best practices for obtaining biological realistic projections. Divers Distrib 261:7671779 Google Scholar
Larkin, DJ, Freyman, MJ, Lishawa, SC, Geddes, P, Tuchman, NC (2012) Mechanisms of dominance by the invasive hybrid cattail Typha× glauca . Biol Invasions 14:6577 CrossRefGoogle Scholar
López-Lanús, B (2016) A case of straight-billed reedhaunter (Limnoctites rectirostris: Furnariidae) nesting in teasel (Dipsacus fullonum: Dipsacoideae). Rev Bras Ornitol 24:211212 CrossRefGoogle Scholar
Mayonde, SG, Cron, V, Gaskin, JF, Byrne, MJ (2016) Tamarix (Tamaricaceae) hybrids: the dominant invasive genotype in southern Africa. Biol Invasions 18:35753594 CrossRefGoogle Scholar
Missouri Botanical Garden (2023) Tropicos. Accessed: November 16, 2023Google Scholar
Moody, ML, Les, DH, Ditomaso, JM (2008) The role of plant systematics in invasive aquatic plant management. J Aquat Plant Manag 46:715 Google Scholar
Mortensen, DA, Bastiaans, L, Sattin, M (2000) The role of ecology in the development of weed management systems: an outlook. Weed Res 40:4962 CrossRefGoogle Scholar
Natural History Museum (2013) Dipsacus spp. In: BSBI List of British & Irish Vascular Plants and Stoneworts. Accessed: November 16, 2023Google Scholar
Neubert, MG, Caswell, H (2000) Demography and dispersal: calculation and sensitivity analysis of invasion speed for structured populations. Ecology 81:16131628 CrossRefGoogle Scholar
Peakall, R, Smouse, PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288295 CrossRefGoogle Scholar
Pritchard, JK, Stephens, M, Donnelly, P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945959 CrossRefGoogle ScholarPubMed
R Core Team (2023) R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing. Google Scholar
Rector, BG, Harizanova, V, Sforza, R, Widmer, T, Wiedenmann, RN (2006) Prospects for biological control of teasels, Dipsacus spp., a new target in the United States. Biol Control 36:114 CrossRefGoogle Scholar
Ringold, PL, Magee, TK, Peck, DV (2008) Twelve invasive plant taxa in US western riparian ecosystems. J N Am Benthol Soc 27:949966 CrossRefGoogle Scholar
Rohlf, FJ (1992) NTSYS-PC: Numerical Taxonomy and Multivariate Analysis System. Setauket, NY: Exeter SoftwareGoogle Scholar
Schierenbeck, KA, Ellstrand, NC (2009) Hybridization and the evolution of invasiveness in plants and other organisms. Biol Invasions 11:10931105 CrossRefGoogle Scholar
Skultety, D, Matthews, JW (2017) Urbanization and roads drive non-native plant invasion in the Chicago Metropolitan region. Biol Invasions 19:25532566 CrossRefGoogle Scholar
Snyder, D, Kaufman, SR (2004) An Overview of Nonindigenous Plant Species in New Jersey. Trenton, NJ: New Jersey Department of Environmental Protection, Division of Parks and Forestry, Office of Natural Lands Management, Natural Heritage Program. 107 pGoogle Scholar
Solecki, MK (1993) Cut-leaved and common teasel (Dipsacus laciniatus L. and D. sylvestris Huds.): profile of two invasive aliens. Pages 85–92 in McKnight BN, ed. Biological Pollution: The Control and Impact of Invasive Exotic Species. Indianapolis: Indiana Academy of SciencesGoogle Scholar
Stoner, WN (1951) An aphid-transmitted virus disease of fuller’s teasel and pincushion flower. Phytopathology 41:191194 Google Scholar
Temsch, EM, Greilhuber, J (2010) Genome size in Dipsacaceae and Morina longifolia (Morinaceae). Plant Syst Evol 289:4556 CrossRefGoogle Scholar
Topham, PN (1968) The Fuller’s teasel. Proc Bot Soc Brit Isl 7:377381 Google Scholar
[USDA-NRCS] U.S. Department of Agriculture–Natural Resources Conservation Service (2023) The PLANTS Database. Greensboro, NC: National Plant Data Team. Accessed: November 16, 2023Google Scholar
[USDI-NPS] U.S. Department of the Interior–National Park Service (2003) Alien Plant Invaders of Natural Areas. Accessed: November 16, 2023Google Scholar
Verlaque, R (1985) Etude biosystematique et phylogenetique des Dipsacaceae. III. Tribus des Knautieae et des Dipsaceae. Rev Cytol Biol Veget-Bot 8:171243 Google Scholar
Vos, P, Hogers, R, Bleeker, M, Reijans, M, van de Lee, T, Hornes, M, Frijters, A, Pot, J, Peleman, J, Kuiper, M (1995) AFLP: a new technique for DNA-fingerprinting. Nucleic Acids Res 23:44074414 CrossRefGoogle ScholarPubMed
Ward, SM, Gaskin, JF, Wilson, LM (2008) Ecological genetics of plant invasion: what do we know? Invasive Plant Sci Manag 1:98109 CrossRefGoogle Scholar
Weber, E (2003) Invasive Plant Species of the World: A Reference Guide to Environmental Weeds. Cambridge, MA: CABI. 548 pGoogle Scholar
Werner, PA (1975) The biology of Canadian weeds. 12. Dipsacus sylvestris Huds. Can J Plant Sci 55:783794 CrossRefGoogle Scholar
White, TJ, Bruns, TD, Lee, SB, Taylor, JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pages 315322 in Innis, MA, Gelfand, DH, Sninsky, JJ, White, TJ, eds. PCR Protocols: A Guide to Methods and Applications. San Diego, CA: Academic Press Google Scholar
Williams, WI, Friedman, JM, Gaskin, JF, Norton, AP (2014) Hybridization of an invasive shrub affects tolerance and resistance to defoliation by a biological control agent. Evol Appl 7:381393 CrossRefGoogle ScholarPubMed
Wringe, BF, Stanley, RR, Jeffery, NW, Anderson, EC, Bradbury, IR (2016) parallelnewhybrid: an R package for the parallelization of hybrid detection using new hybrids. Mol Ecol Res 17:9195 CrossRefGoogle Scholar
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