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Rethinking Invasion Impacts across Multiple Field Sites Using European Swallowwort (Vincetoxicum rossicum) as a Model Invader

  • Grant L. Thompson (a1), Terrence H. Bell (a2) and Jenny Kao-Kniffin (a3)

European swallowwort [Vincetoxicum rossicum (Kleopow) Barbarich] is found in the northeastern United States and southeastern Canada. It forms dense growth patterns that reduce plant and insect biodiversity, and lab assays show that it produces allelopathic compounds that affect microbial activity. Consequently, we hypothesized that V. rossicum alters soil microbiome composition and activity in invaded habitats, which may impact ecosystem properties and processes. We sampled soil from a similar time point within a growing season at each of five sites in New York State where V. rossicum was both present and absent. We measured bacterial and fungal microbiome composition, available soil nitrogen (N), soil respiration (CO2 flux), and soil extracellular enzyme activities. Microbial composition varied across field sites, but only fungal composition was affected by invasion. No significant differences were found between the invaded and uninvaded plots at any of the sites for available soil ammonium, nitrate, or respiration, though extractable N varied greatly between sites. Microbial hydrolytic extracellular enzyme activities suggest decreased protein degradation and increased oxidative enzyme activity with V. rossicum invasion, which is relevant to soil N and carbon cycling processes. Although V. rossicum impacted rhizosphere microbial composition and activity, it was not associated with large perturbations in ecosystem function when examined across multiple invasion sites during this short-term study.

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      Rethinking Invasion Impacts across Multiple Field Sites Using European Swallowwort (Vincetoxicum rossicum) as a Model Invader
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Corresponding author
*Author for correspondence: J. Kao-Kniffin, Section of Horticulture, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853. (Email:
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Allison, SD, Gartner, T, Holland, K, Weintraub, M, Sinsabaugh, RL (2007) Soil enzymes: linking proteomics and ecological process. Pages 704711 in Manual of Environmental Microbiology. Washington, DC: American Society of Microbiology
Averill, KM, DiTommaso, A, Mohler, CL, Milbrath, LR (2010) Establishment of the invasive perennial Vincetoxicum rossicum across a disturbance gradient in New York State, USA. Plant Ecol 211:6577
Bell, TH, Cloutier-Hurteau, B, Al-Otaibi, F, Turmel, MC, Yergeau, E, Courchesne, F, St-Arnaud, M (2015) Early rhizosphere microbiome composition is related to the growth and Zn uptake of willows introduced to a former landfill. Environ Microbiol 17:30253038
Bell, TH, Hassan, SE-D, Lauron-Moreau, A, Al-Otaibi, F, Hijri, M, Yergeau, E, St-Arnaud, M (2014) Linkage between bacterial and fungal rhizosphere communities in hydrocarbon-contaminated soils is related to plant phylogeny. ISME J 8:331343
Bell, TH, Yergeau, E, Maynard, C, Juck, D, Whyte, LG, Greer, CW (2013) Predictable bacterial composition and hydrocarbon degradation in Arctic soils following diesel and nutrient disturbance. ISME J 7:1200
Callaway, RM, Cipollini, D, Barto, K, Thelen, GC, Hallett, SG, Prati, D, Stinson, K, Klironomos, J (2008) Novel weapons: invasive plant suppresses fungal mutualists in America but not in its native Europe. Ecology 89:10431055
Cappuccino, N, Arnason, JT (2006) Novel chemistry of invasive exotic plants. Biol Lett 2:189193
Cappuccino, N, Mackay, R, Eisner, C (2002) Spread of the invasive alien vine Vincetoxicum rossicum: tradeoffs between seed dispersability and seed quality. Am Midl Nat 148:263270
Cassman, NA, Leite, MF, Pan, Y, De Hollander, M, Van Veen, JA, Kuramae, EE (2016) Plant and soil fungal but not soil bacterial communities are linked in long-term fertilized grassland. Sci Rep 6:23680
CastroDíez, P, Godoy, O, Alonso, A, Gallardo, A, Saldaña, A (2014) What explains variation in the impacts of exotic plant invasions on the nitrogen cycle? A metaanalysis. Ecol Lett 17:112
DiTommaso, A, Lawlor, FM, Darbyshire, SJ (2005) The biology of invasive alien plants in Canada. 2. Cynanchum rossicum (Kleopow) Borhidi [= Vincetoxicum rossicum (Kleopow) Barbar.] and Cynanchum louiseae (L.) Kartesz & Gandhi [= Vincetoxicum nigrum (L.) Moench]. Can J Plant Sci 85:243263
Douglass, CH, Weston, LA, Wolfe, D (2010) Phytotoxicity and potential allelopathy in pale (Cynanchum rossicum) and black swallowwort (C. nigrum). Invasive Plant Sci Manag 4:133141
Drinkwater, LE, Cambardella, CA, Reeder, JD, Rice, CW (1996) Potentially mineralizable nitrogen as an indicator of biologically active soil nitrogen. Pages 217229 in Doran JW, Jones AJ, eds . Methods for Assessing Soil Quality. Madison, WI: Soil Science Society of America
Ehrenfeld, JG (2003) Effects of exotic plant invasions on soil nutrient cycling processes. Ecosystems 6:503523
Ehrenfeld, JG (2010) Ecosystem consequences of biological invasions. Annu Rev Ecol Evol Syst 41:5980
Elgersma, K, Ehrenfeld, J, Yu, S, Vor, T (2011) Legacy effects overwhelm the short-term effects of exotic plant invasion and restoration on soil microbial community structure, enzyme activities, and nitrogen cycling. Oecologia 167:733745
Gardes, M, Bruns, TD (1993) ITS primers with enhanced specificity for basidiomycetesapplication to the identification of mycorrhizae and rusts. Mol Ecol 2:113118
German, DP, Weintraub, MN, Grandy, AS, Lauber, CL, Rinkes, ZL, Allison, SD (2011) Optimization of hydrolytic and oxidative enzyme methods for ecosystem studies. Soil Biol Biochem 43:13871397
Gibson, D, Krasnoff, S, Biazzo, J, Milbrath, L (2011) Phytotoxicity of antofine from invasive swallow-worts. J Chem Ecol 37:871879
Gibson, DM, Vaughan, RH, Milbrath, LR (2015) Invasive swallow-worts: an allelopathic role for-(−) antofine remains unclear. J Chem Ecol 41:202211
Herlemann, DP, Labrenz, M, Jürgens, K, Bertilsson, S, Waniek, JJ, Andersson, AF (2011) Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea. ISME J 5:15711579
Howard, MM, Bell, TH, Kao-Kniffin, J (2017) Soil microbiome transfer method affects microbiome composition, including dominant microorganisms, in a novel environment. FEMS Microbiol Lett 364, 10.1093/femsle/fnx092
Kourtev, P, Ehrenfeld, J, Huang, W (2002) Enzyme activities during litter decomposition of two exotic and two native plant species in hardwood forests of New Jersey. Soil Biol Biochem 34:12071218
Li, W, Zhang, C, Peng, C (2012) Responses of soil microbial community structure and potential mineralization processes to Solidago canadensis invasion. Soil Sci 177:433442
Martin, KJ, Rygiewicz, PT (2005) Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts. BMC Microbiol 5:28
Mogg, C, Petit, P, Cappuccino, N, Durst, T, McKague, C, Foster, M, Yack, JE, Arnason, JT, Smith, ML (2008) Tests of the antibiotic properties of the invasive vine Vincetoxicum rossicum against bacteria, fungi and insects. Biochem Syst Ecol 36:383391
Pylro, VS, Roesch, LFW, Morais, DK, Clark, IM, Hirsch, PR, Tótola, MR (2014) Data analysis for 16S microbial profiling from different benchtop sequencing platforms. J Microbiol Methods 107:3037
Saiya-Cork, K, Sinsabaugh, R, Zak, D (2002) The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biol Biochem 34:13091315
Schimel, JP, Bennett, J (2004) Nitrogen mineralization: challenges of a changing paradigm. Ecology 85:591602
Schloss, PD, Westcott, SL, Ryabin, T, Hall, JR, Hartmann, M, Hollister, EB, Lesniewski, RA, Oakley, BB, Parks, DH, Robinson, CJ (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:75377541
Sinsabaugh, RL (2010) Phenol oxidase, peroxidase and organic matter dynamics of soil. Soil Biol Biochem 42:391404
Sinsabaugh, RL, Gallo, ME, Lauber, C, Waldrop, MP, Zak, DR (2005) Extracellular enzyme activities and soil organic matter dynamics for northern hardwood forests receiving simulated nitrogen deposition. Biogeochemistry 75:201215
Sinsabaugh, RL, Lauber, CL, Weintraub, MN, Ahmed, B, Allison, SD, Crenshaw, C, Contosta, AR, Cusack, D, Frey, S, Gallo, ME, Gartner, TB, Hobbie, SE, Holland, K, Keeler, BL, Powers, JS, Stursova, M, Takacs-Vesbach, C, Waldrop, MP, Wallenstein, MD, Zak, DR, Zeglin, LH (2008) Stoichiometry of soil enzyme activity at global scale. Ecol Lett 11:12521264
Smith, LL, DiTommaso, A, Lehmann, J, Greipsson, S (2008) Effects of arbuscular mycorrhizal fungi on the exotic invasive vine pale swallow-wort (Vincetoxicum rossicum). Invasive Plant Sci Manag 1:142152
Stockinger, H, Krüger, M, Schüßler, A (2010) DNA barcoding of arbuscular mycorrhizal fungi. New Phytol 187:461474
[USDA-NRCS] (2014) U.S. Department of Agriculture, Natural Resources Conservation Service. Web Soil Survey. Accessed: April 30, 2018
Vitousek, PM, Walker, LR, Whiteaker, LD, Mueller-Dombois, D, Matson, PA (1987) Biological invasion by Myrica faya alters ecosystem development in Hawaii. Science 238:802804
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