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Changes in plasma palmitate specific radioactivity during long-term intravenous infusions of tritiated palmitate into lactating goats

Published online by Cambridge University Press:  27 March 2009

Barbara A. König ,
J. D. Oldham  and
D. S. Parker
Barbara A. König
Affiliation:
Department of Physiology and Biochemistry, The University, Whiteknights, Reading, RO6 2AJ
J. D. Oldham
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RO2 9AT
D. S. Parker
Affiliation:
Department of Physiology and Biochemistry, The University, Whiteknights, Reading, RO6 2AJ
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Extract

Vascular injection or infusion of isotopically labelled fatty acids into both ruminant and nonruminant species has been used as a method for determining the entry rate of non-esterified fatty acids (NEFA) into blood (Bickerstaffe, Annison & Linzell, 1974; Vranic, 1975). Results obtained in this way represent the release of fatty acids from several sources, principally adipose tissue. The predominant labelled end-products from [3H]– and [14C]fatty acid metabolism are water and carbon dioxide respectively. Both these metabolites enter extensive body pools and the label is unlikely to be reincorporated into plasma NEFA during the time course of conventional short-term experiments (2–4 h). During isotope dilution experiments, however, some labelled fatty acid could be incorporated into adipose tissue triacyglycerol (TAG) following synthesis of low-density lipoprotein in the liver. In addition, the contribution of NEFA carbon to endogenous acetate production could result in transfer of 14C in any C, or 8H attached to the C8 position in acetate, from the infused fatty acid to fatty acids synthesized by liver and adipose tissue.

Type
Short Note
Information
The Journal of Agricultural Science , Volume 101 , Issue 3 , December 1983 , pp. 763 - 765
Copyright
Copyright © Cambridge University Press 1983

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