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The effects of compound treatment of Aspergillus oryzae and fibrolytic enzyme on in vitro degradation, gas production and fermentative profile of maize silage and sugarcane silage

Published online by Cambridge University Press:  21 May 2021

J. M. Souza Open the ORCID record for J. M. Souza [Opens in a new window] ,
J. C. S. M. Souza Open the ORCID record for J. C. S. M. Souza [Opens in a new window] ,
D. O. Sousa ,
T. A. Del Valle ,
L. G. Ghizzi ,
A. H. D. Alcântara ,
L. G. Mesquita ,
R. L. M. Sousa ,
I. C. S. Bueno  and
J. C. C. Balieiro
J. M. Souza*
Affiliation:
Faculty of Veterinary Medicine and Animal Science, Department of Animal Nutrition and Animal Production, University of São Paulo, Pirassununga13635-900, Brazil
J. C. S. M. Souza
Affiliation:
Faculty of Veterinary Medicine and Animal Science, Department of Animal Nutrition and Animal Production, University of São Paulo, Pirassununga13635-900, Brazil
D. O. Sousa
Affiliation:
Department of Animal Environment and Health, Swedish University of Agricultural Sciences, Skara532 23, Sweden
T. A. Del Valle
Affiliation:
Federal University of Pampa, Itaqui96460-000, Brazil
L. G. Ghizzi
Affiliation:
Faculty of Veterinary Medicine and Animal Science, Department of Animal Nutrition and Animal Production, University of São Paulo, Pirassununga13635-900, Brazil
A. H. D. Alcântara
Affiliation:
Faculty of Animal Science and Food Engineering, Department of Animal Science, University of São Paulo, Pirassununga13635-900, Brazil
L. G. Mesquita
Affiliation:
Faculty of Veterinary Medicine and Animal Science, Department of Animal Nutrition and Animal Production, University of São Paulo, Pirassununga13635-900, Brazil
R. L. M. Sousa
Affiliation:
Faculty of Animal Science and Food Engineering, Department of Animal Science, University of São Paulo, Pirassununga13635-900, Brazil
I. C. S. Bueno
Affiliation:
Faculty of Animal Science and Food Engineering, Department of Animal Science, University of São Paulo, Pirassununga13635-900, Brazil
J. C. C. Balieiro
Affiliation:
Faculty of Veterinary Medicine and Animal Science, Department of Animal Nutrition and Animal Production, University of São Paulo, Pirassununga13635-900, Brazil
*
Author for correspondence: J. M. Souza, Email: souza.jjm@gmail.com
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Abstract

The present study was conducted to evaluate the effect of a live culture of Aspergillus oryzae (A; CCT4359) and fibrolytic enzyme (E; Fibrozyme Alltech Inc.) on fibre digestibility by a gas production bioassay and in vitro degradation of maize silage and sugarcane silage. A completely randomized design trial was performed to evaluate: A doses (0, 20, 60 and 100 mg/l), E doses (0, 160, 320 and 480 mg/l) and roughage source (R; maize and sugarcane silage) in a 4 × 4 × 2 factorial arrangement. The inclusion of increasing doses of A and E increased dry matter and neutral detergent fibre in vitro digestibility linearly, but for E this effect occurred only in maize silage. There was a linear increase in the potential for gas production at the highest dose of A only in sugarcane silage, with no effect on lag time (L). Increasing doses of E increased the volume of gases produced linearly, and a trend of linear reduction of L, regardless of the roughage. There was a linear reduction in ammonia-nitrogen concentration in response to increasing doses of A and E, and an increase in acetic acid concentration at the highest dose of A, regardless of roughage. The additives had no synergistic effect on gas production and digestibility, but were efficient in altering the fermentative pattern, demonstrating the potential to increase fibre degradation.

Keywords

Additivesdirect-fed microbialsfibre digestibilityin vitro bioassayruminants
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 159 , Issue 1-2 , January 2021 , pp. 147 - 158
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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