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The fate of acetyl groups and sugar components during the digestion of grass cell walls in sheep

Published online by Cambridge University Press:  27 March 2009

E. Jane Morris  and
J. S. D. Bacon
E. Jane Morris
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB
J. S. D. Bacon
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB
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Summary

The digestibilities of grass cell wall constituents determined in a digestion trial were compared with those obtained by suspending various isolated cell wall preparations in nylon bags in the rumen of a sheep. Particular attention was paid to acetyl groups and to individual sugars, which were determined in both cases by gas liquid chromatography.

For dried grass and hay in the digestion trial the cell wall constituents showed digestibilities decreasing in the following order: arabinose, galactose, glucose, xylose, acetyl, lignin.

For a leaf cell wall preparation derived from all cell types except mesophyll, the nylon bag technique allowed the same order of digestibilities; rhamnose and uronic acids were also measured and found to be rapidly digested. Mesophyll cell walls placed in nylon bags were more readily digested than non-mesophyll. All the sugars, and also acetyl groups, were digested to the same extent.

In a grass cell wall preparation isolated from sheep faeces, tested similarly, xylose and glucose were digested to the same extent, but acetyl groups were less digested.

Removal of acetyl groups, using sodium ethoxide, which left the sugar composition and lignin content unchanged, increased the digestibility particularly of the cell walls from faeces.

The results are discussed with reference to the relationship between cell wall composition and digestibility.

Information

Type
Research Article
Information
The Journal of Agricultural Science , Volume 89 , Issue 2 , October 1977 , pp. 327 - 340
Copyright
Copyright © Cambridge University Press 1977

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References

Akin, D. E., Amos, H. E., Barton, F. E. & Burdick, D. (1973). Rumen microbial degradation of grass tissue revealed by scanning electron microscopy. Agronomy Journal 65, 825–8.Google Scholar
Armstrong, D. G. & Beever, D. E. (1969). Postabomasal digestion of carbohydrate in the adult ruminant. Proceedings of the Nutrition Society 28, 121–31.Google Scholar
Ashwell, G. (1957). In Methods in Enzymology (ed. Colowick, S. P. and Kaplan, N. O.), vol. III, pp. 73–105. New York: Academic Press.Google Scholar
Bacon, J. S. D., Gordon, A. H., Morris, E. J. & Farmer, V. C. (1975). Acetyl groups in cell wall preparations from higher plants. Biochemical Journal 149, 485–7.CrossRefGoogle ScholarPubMed
Bacon, J. S. D., Gordon, A. H. & Hay, A. J. (1976). Acetyl groups in dietary polysaccharides. Proceedings of the Nutrition Society 35, 93A.Google ScholarPubMed
Bailey, R. W., Allison, R. M. & O'Connor, K. F. (1971). Protein and carbohydrate composition of lucerne grown in Canterbury. Proceedings of the New Zealand Grassland Association 32, 127–36.Google Scholar
Bethge, P. O. & Lindstrom, K. (1973). Determination of O-acetyl groups in wood. Svensk Papperstidning 76, 645–9.Google Scholar
Beveridge, R. J. & Richards, G. N. (1973). Digestion of polysaccharide constituents of tropical pasture herbage in the bovine rumen. Part III. Examination of the celluloses and hemicelluloses of spear grass (Heteropogon contortus) which resist digestion. Carbohydrate Research 28, 39–43.CrossRefGoogle Scholar
Blumenkrantz, N. & Asboe-Hansen, G. (1973). New method for quantitative determination of uronic acids. Analytical Biochemistry 54, 484–9.Google Scholar
Bouveng, H. O. (1961). Phenylisocyanate derivatives of carbohydrates. II. Location of the O-acetyl groups in birch xylan. Acta Chemica Scandinavica 15, 96–100.Google Scholar
Christian, K. (1971). Detergent method for total lignin in herbage. Field Station Records, Division of Plant Industry CSIRO Australia 10, 29–34.Google Scholar
Dekker, R. F. H., Richards, G. N. & Playne, M. J. (1972). Digestion of polysaccharide constituents of tropical pasture herbage in the bovine rumen. Part I. Townsville Stylo (Stylosanthes humilis). Carbohydrate Research 22, 173–85.CrossRefGoogle Scholar
Gaillard, B. D. E. & Richards, G. N. (1975). Presence of soluble lignin-carbohydrate complexes in the bovine rumen. Carbohydrate Research 42, 135–45.Google Scholar
Gaillard, B. D. E. & Van't Klooster, A. T. (1973). Observations on the fermentation of carbohydrates along the gastro-intestinal tract of a fistulated cow. Netherlands Journal of Agricultural Science 21, 217–26.CrossRefGoogle Scholar
Gaussères, B. (1965). Étude des variations des teneurs en glucides cytoplasmiques et membranaires du dactyle au cours de l'année. Annales de Biologie Animale, Biochimie, Biophysique 5, 361–81.CrossRefGoogle Scholar
Gordon, A. H., Hay, A. J., Dinsdale, D. & Bacon, J. S. D. (1977). Polysaccharides and associated components of mesophyll cell walls prepared from grasses. Carbohydrate Research. (In the Press.)Google Scholar
Hanna, W. W., Monson, W. G. & Burton, G. W. (1973). Histological examination of fresh forage leaves after in vitro digestion. Crop Science 13, 98–102.CrossRefGoogle Scholar
Hartley, R. D. (1973). Carbohydrate esters of ferulic acid as components of cell walls of Lolium multiflorum. Phytochemistry 12, 661–5.Google Scholar
Hopson, J. D., Johnson, R. R. & Dehority, B. A. (1963). Evaluation of the dacron bag technique as a method for measuring cellulose digestibility and rate of forage digestion. Journal of Animal Science 22, 448–53.Google Scholar
Howard, B. H. (1957). Hydrolysis of the soluble pentosans of wheat flour and Rhodymenia palmata by ruminal micro-organisms. Biochemical Journal 67, 643–50.Google Scholar
Jarrige, R. (1960). The membrane constituents of herbage. Proceedings of the 8th International Grassland Congress 628–35.Google Scholar
Jarrige, R. (1963). Les constituants membranaires des plantes fourragères. Annales de Biologie Animale, Biochimie, Biophysique 3, 143–90.Google Scholar
Mackenzie, D. J. & Wylam, C. B. (1957). Analytical studies on the carbohydrates of grasses and clovers. VIII. Changes in carbohydrate composition during the growth of perennial ryegrass. Journal of the Science of Food and Agriculture 8, 38–45.CrossRefGoogle Scholar
Monson, W. G. & Burton, G. L. (1972). Effects of length of cut and leaf surface treatments on digestibility of fresh forage. Agronomy Journal 64, 405–6.Google Scholar
Morris, E. J. (1976). Acetyl content and sugar composition as factors influencing the digestion of grass cell walls in the rumen. Ph.D. Thesis, University of Aberdeen.Google Scholar
Morris, E. J. & Bacon, J. S. D. (1976). Digestion of acetyl groups and cell wall polysaccharides of grasses in the rumen. Proceedings of the Nutrition Society 35, 94A.Google ScholarPubMed
Morrison, I. M. (1972). Semi-micro method for determination of lignin and its use in predicting digestibility of forage crops. Journal of the Science of Food and Agriculture 23, 453–63.CrossRefGoogle ScholarPubMed
Regal, V. (1960). The evaluation of the quality of pasture grasses by the microscopic method. Proceedings of the 8th International Grassland Congress 522–4.Google Scholar
Selim, O. I., Wilson, D. & Jones, D. I. H. (1975). A histological technique, using cellulolytic enzyme digestion, for assessing nutritive quality differences in grasses. Journal of Agricultural Science, Cambridge 85, 297–9.Google Scholar
Sloneker, J. H. (1971). Determination of cellulose and apparent hemicellulose in plant tissue by gas-liquid chromatography. Analytical Biochemistry 43, 539–46.Google Scholar
Sloneker, J. H. (1972). In Methods in Carbohydrate Chemistry (ed. Whistler, R. J. and Be Miller, J. N.), vol. VI, pp. 20–4, New York: Academic Press.Google Scholar
Smith, E. L. (1927). A process for the dehydration of alcohol. Journal of the Chemical Society 1288–90.CrossRefGoogle Scholar
Ulyatt, M. J. & Macrae, J. C. (1974). Quantitative digestion of fresh herbage by sheep. 1. The sites of digestion of organic matter, energy, readily fermentable carbohydrate, structural carbohydrate, and lipid. Journal of Agricultural Science, Cambridge 82, 295–307.Google Scholar
Updegraff, D. M. (1969). Semimicro determination of cellulose in biological materials. Analytical Biochemistry 32, 420–4.Google Scholar
Van Soest, P. J. (1963). Use of detergents in the analysis of fibrous feeds. II. A rapid method for the determination of fibre and lignin. Journal of the Association of Official Agricultural Chemists 46, 829–35.Google Scholar
Van Soest, P. J. (1969). The chemical basis for the nutritive evaluation of forages. Proceedings of National Conference on Forage Quality Evaluation and Utilization, U1–U19.Google Scholar
Van Soest, P. J. (1973). The uniformity and nutritive availability of cellulose. Federation Proceedings 32, 1804–8.Google Scholar
Van Soest, P. J. & Wine, R. H. (1967). Use of detergents in the analysis of fibrous feeds. IV. Determination of plant cell-wall constituents. Journal of the Association of Official Agricultural Chemists 50, 50–5.Google Scholar
Waite, R. & Gorrod, A. R. N. (1959). The structural carbohydrates of grasses. Journal of the Science of Food and Agriculture 10, 308–17.Google Scholar
Waite, R., Johnston, M. J. & Armstrong, D. G. (1964). The evaluation of artificially dried grass as a source of energy for sheep. 1. The effect of stage of maturity on the apparent digestibility of rye-grass, cocksfoot and timothy. Journal of Agricultural Science, Cambridge 62, 391–8.Google Scholar