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Identification and fine mapping of lemma-distortion1, a single recessive gene playing an essential role in the development of lemma in rice

Published online by Cambridge University Press:  19 January 2016

D. W. YANG ,
X. F. YE ,
X. H. ZHENG ,
C. P. CHENG ,
N. YE ,
L. B. LU ,
F. H. HUANG  and
Q. Q. LI
D. W. YANG
Affiliation:
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fujian High Quality Rice Research & Development Center, Fuzhou 350019, China
X. F. YE*
Affiliation:
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fujian High Quality Rice Research & Development Center, Fuzhou 350019, China
X. H. ZHENG
Affiliation:
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fujian High Quality Rice Research & Development Center, Fuzhou 350019, China
C. P. CHENG
Affiliation:
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fujian High Quality Rice Research & Development Center, Fuzhou 350019, China
N. YE
Affiliation:
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fujian High Quality Rice Research & Development Center, Fuzhou 350019, China
L. B. LU
Affiliation:
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fujian High Quality Rice Research & Development Center, Fuzhou 350019, China
F. H. HUANG
Affiliation:
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fujian High Quality Rice Research & Development Center, Fuzhou 350019, China
Q. Q. LI*
Affiliation:
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fujian High Quality Rice Research & Development Center, Fuzhou 350019, China
*
*To whom all correspondence should be addressed. Emails: yexinfu@126.com; liqq@xmu.edu.cn
*To whom all correspondence should be addressed. Emails: yexinfu@126.com; liqq@xmu.edu.cn
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Summary

Floral organ development influences plant reproduction and crop yield. The mechanism of floral organ specification is generally conserved in angiosperms as demonstrated by the ‘ABC’ model. However, mechanisms underlying the development of floral organs in specific groups of species such as grasses remain unclear. In the genus Oryza (rice), a spikelet consists of a fertile floret sub-tended by a lemma, a palea, two sterile lemmas and rudimentary glumes. To understand how the lemma is formed, a curve-shaped lemma-distortion1 (ld1) mutant was identified. Genetic analysis confirmed that the ld1 mutant phenotype was due to a single recessive gene mutation. Using a large F2 population, the LD1 gene was mapped between markers Indel-7-15 and Indel-7-18, which encompassed a region of 15·6 kilo base pairs (kbp). According to rice genome annotations, two putative genes, LOC_Os07g32510 and LOC_Os07g32520, were located in this candidate region. However, DNA sequencing results indicated only 1 base pair (bp) substitution (T⇨C) was found in LOC_Os07g32510 between the wild-type and the ld1 mutant. Thus LOC_Os07g32510, encoding a DNA binding with one zinc finger (DoF) containing protein, was the candidate gene for LD1. Further analysis showed that mutation of the amino acid cysteine (C) to arginine (R) was likely to lead to zinc finger protein deactivation. Phylogenetic and conservation analysis of the gene from different species revealed that cysteine was critical to LD1 function. As a new gene controlling lemma development, the study of LD1 could provide insights into rice floral organ formation mechanisms.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 154 , Issue 6 , August 2016 , pp. 989 - 1001
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Copyright
Copyright © Cambridge University Press 2016 

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