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Spikelet fertility and heat shock transcription factor (Hsf) gene responses to heat stress in tolerant and susceptible rice (Oryza sativa L.) genotypes

Published online by Cambridge University Press:  29 August 2019

C. Malumpong Open the ORCID record for C. Malumpong [Opens in a new window] ,
S. Cheabu ,
C. Mongkolsiriwatana ,
W. Detpittayanan  and
A. Vanavichit
C. Malumpong*
Affiliation:
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom 73140, Thailand Rice Science Center & Rice Gene Discovery Unit, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand
S. Cheabu
Affiliation:
Rice Science Center & Rice Gene Discovery Unit, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand Faculty of Agriculture, Princess of Naradhiwas University, Naradhiwas 96000, Thailand
C. Mongkolsiriwatana
Affiliation:
Division of Genetics, Department of Science, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Sean Campus, Nakron Pathom 73140, Thailand
W. Detpittayanan
Affiliation:
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom 73140, Thailand
A. Vanavichit
Affiliation:
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom 73140, Thailand Rice Science Center & Rice Gene Discovery Unit, Kasetsart University, Kamphaeng Sean Campus, Nakhon Pathom 73140, Thailand
*
Author for correspondence: C. Malumpong, E-mail: agrcnm@ku.ac.th
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Abstract

The reproductive stage of rice is the most sensitive to heat stress, which can lead to spikelet sterility. Thus, heat-tolerant and heat-susceptible genotypes were used to investigate their differences in terms of phenotypic responses and expression changes of Hsf genes at the pre-flowering stage under heat stress. Results clearly showed that panicles had the highest temperature compared with other plant parts under both natural and heated conditions. However, the temperatures of tolerant and susceptible genotypes were not significantly different. In terms of spikelet fertility, the tolerant lines M9962 and M7988 had high seed set because their anther dehiscence, pollen viability and pollen germination were only slightly affected. In contrast, the susceptible line Sinlek showed severe effects at all steps of fertilization, and the pollen viability of M7766 was slightly affected under heat stress but was more affected in terms of anther dehiscence and pollen germination. Both susceptible lines showed dramatically decreased seed set. In addition, the expression of six HsfA genes in the flag leaves and spikelets at the R2 stage of plants under heat stress showed different responses. Notably, expression of the HsfA2a gene was predominantly upregulated in the flag leaf and spikelets under heat stress in M9962. Therefore, it can be concluded that heat stress has severe effects on the stamen, and that different genotypes have different susceptibilities to heat stress.

Keywords

Heat shock transcription factorheat stressricespikelet fertility

Information

Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 157 , Issue 4 , May 2019 , pp. 283 - 299
Copyright
Copyright © Cambridge University Press 2019 

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