Introduction
As emerging artificial intelligence (AI) technology continues to drive healthcare and biomedical education forward, there lies a significant challenge to translate innovative advancements into practical tools that meet the needs of students with varying skill levels and backgrounds. Utilized effectively, AI has the power to transform medical and biomedical education, providing collaborative, tailored learning opportunities. However, educators may struggle to keep up with the rapid pace of technological development and how best to incorporate AI-powered technologies into undergraduate curricula.
Learning objectives are the anticipated knowledge and skills that students should obtain after a period of learning [Reference Osueke, Mekonnen and Stanton1–Reference Allan3] and are distinct from learning outcomes, which are the observed knowledge and skills that students have obtained [17,Reference Owen18,Reference Sutrop20]. Engaging students in developing learning objectives can help increase awareness of connections between classroom-acquired knowledge and its real-world application when they become part of the biomedical workforce [Reference Singh, Ferry and Mills4]. Thus, engaging students in developing learning objectives for AI technologies can enhance their competencies in using AI-powered applications and equip them with the necessary skills to navigate the AI-driven future of medicine. Additionally, students from different backgrounds, disciplines, and stages will demonstrate different learning motivations and competencies. Thus, participatory co-design efforts help ensure that curricula are tailored to their diverse needs.
Previous examples of the successful engagement of students in the development of learning objectives include the involvement of medical students in incorporating informatics learning objectives into medical school curricula [Reference Beaudoin, Richardson, Sheng and Mitchell5] and the development of active learning components in biomedical engineering courses across laboratory, classroom, simulation-based clinical, and clinically immersive real-world settings. Student engagement in identifying learning outcomes was linked to enhanced learning and trainee perceptions of adaptive, experiential learning environments [Reference Singh, Ferry and Mills4]. Engaging students in the development and articulation of learning objectives thus ensures that the learning objectives are designed to meet students’ self-perceived needs, helping them to achieve their learning outcomes and setting them up for success in the workforce.
Objectives
This study aimed to engage undergraduate students in the development of learning objectives for Functional Unit State Identification & Navigation with Whole Slide Images (FUSION), a new AI-powered tissue visualization and analysis technology. Specifically, we sought to enhance student-centered learning by bridging the gap between FUSION’s AI-powered biomedical and technological potential and its implementation into educational environments. Through interviews with students, we expected to identify multiple perceived uses of FUSION. Using cultural domain analysis (CDA), we planned to organize these into clusters of unique statements, which would then inform the development of potential learning objectives. The study set out to answer the following research questions:
RQ1: What applications for an AI-powered tissue visualization and analysis technology are salient among undergraduate students?
RQ2: What are trainee-centered learning objectives for undergraduate training in an AI-powered tissue visualization and analysis technology?
Materials and methods
Context
This study explored the usability of a new AI-powered technology, FUSION, which was developed under the Human BioMolecular Atlas Program (HuBMAP) [Reference Jain, Pei and Spraggins10,Reference Border, Ferreira and Lucarelli11]. HuBMAP is funded by the Common Fund at the National Institutes of Health and includes researchers across multiple U.S. and international institutions. HuBMAP’s goal is to create a comprehensive spatial map of the human body at the single-cell level to advance understanding of how cells interact in the human body [Reference Jain, Pei and Spraggins10] To achieve this, HuBMAP is entering its production phase for an infrastructure capable of mapping functional tissue units across different organs [Reference Jain, Pei and Spraggins10]. Under this initiative, the HuBMAP Infrastructure, Visualization, and Engagement team implemented deep learning algorithms, high-performance computing, and spatially resolved molecular omics to create FUSION. Since 2021, HuBMAP has also offered internships for undergraduate students to support biomedical workforce development by creating a pipeline of emerging graduates familiar with HuBMAP tools, data, and processes.
FUSION is an innovative visualization tool for analyzing tissue samples that automates the tracing of structural boundaries within biopsies, enabling the precise measurements of the size, shape, color, and texture of functional tissue units [Reference Border, Ferreira and Lucarelli11,Reference Border, Lucarelli, Eadon, El-Achkar, Jain and Sarder12]. These measurements correlate with gene expression data, allowing researchers to map molecular information to specific areas in a biopsy, bridging the gap between pathology and molecular biology [Reference Border, Ferreira and Lucarelli11,Reference Border, Lucarelli, Eadon, El-Achkar, Jain and Sarder12]. FUSION integrates AI by executing automated segmentation algorithms to extract the boundaries and locations of different structures in histological tissue. For kidney sections, these include glomeruli, globally sclerotic glomeruli, tubules, arteries, and arterioles. Of these structures, tubules are the most numerous, as a single biopsy can contain upwards of 2,000 tubules of several different sub-types. Also included in FUSION, though maybe not traditionally thought of as AI, are a variety of clustering and dimensional reduction algorithms that are used on high-dimensional data to group similar samples under a single broad label. Specifically, FUSION implements various cell deconvolution methods wherein spatial transcriptomics measurements are aligned with a single-nucleus RNA-sequencing reference object and transformed to provide a relative “score” for approximately 72 different kidney cell sub-types. Furthermore, FUSION offers several different methods for expert users (and non-computational users) to generate annotations for different structures within slides or for entire slides. This information can then be used to train machine learning algorithms, which can be deployed as custom components in FUSION.
Consensus-based needs assessment using cultural domain analysis
To assess FUSION’s potential curricular applications and identify salient ideas among biomedical and electrical engineering undergraduate students, we employed CDA, a cognitive anthropology method. CDA aims to “describe the contents, structure, and distribution of knowledge in organized spheres of experience, or cultural domains [Reference Gravlee, Maxwell, Jacobsohn and Bernard13].” CDA is concerned with how people with a shared experience think and talk about it [Reference Albughayl, Beckford, Okoko, Tunison and Walker14]. CDA’s primary purpose is to enable the systematic identification of shared knowledge [Reference Dengah, Brewis and Johnson15], and a “cultural domain” refers to knowledge shared by a group. Identifying cultural domains involves eliciting responses from interviewees or asking respondents to list items that they associate with a domain of understanding in an unconstrained way, called “free-listing [Reference Borgatti and Halgin6,Reference Smith and Borgatti8,Reference Dengah, Snodgrass, Polzer and Nixon9].” Researchers then look for shared patterns among respondents, indicating potentially culturally learned domains [Reference Dengah, Snodgrass, Polzer and Nixon9]. Cultural domains can also be understood as “categories,” for example, animals or illnesses, that are shared and agreed among individuals and are about “perceptions rather than preferences [Reference Borgatti and Halgin6,Reference Ackley, Rodriguez and Villa7].” CDA’s mixed methods approach makes it especially well-suited for generating hypotheses in emerging areas, such as integrating new AI technologies in biomedical education settings. As technology continues to play an ever-increasing role in medical education, there is a growing need to understand students’ shared knowledge and perceptions toward these platforms. By assessing student perceptions, instructors can develop learner-centered, rather than technology-centered, curricula. This assessment can also help map a module using research technology, such as FUSION, into existing applications of knowledge that are familiar to the students. This, in turn, can support the development of skills that span across different technology platforms and address higher-level learning objectives.
Participants
Participants were recruited through emails sent to the listservs of the University of Florida’s biomedical and electrical engineering departments. In addition, we invited undergraduate students who participated in previous summer research experiences hosted by the research team. The invitation email introduced the study’s objectives, emphasized the voluntary nature of participation, and provided details about the $25 incentive. Those who expressed interest were provided further instructions for scheduling an elicitation interview via Zoom. The inclusion of students from both a general convenience sample and those with prior summer research experience was intended to capture a broader range of student experiences, learning motivations, and competencies. Given that students from different backgrounds, disciplines, and stages of training demonstrate different competencies, including students with differing levels of past research experience, it supports the generalizability and real-world applicability of the learning objectives developed in this study.
Procedure
Upon joining the interview session, participants were informed that the interview would be recorded for transcription and analysis and were asked to provide verbal consent before proceeding. Participants received a brief overview of the study’s purpose, procedures, confidentiality protocols, and their rights as participants. The participants were then presented with a short 4-minute video that introduced the capabilities and functionalities of FUSION, ensuring all participants had the same level of understanding of the platform [Reference CMILab16,17].
Following the video presentation, participants engaged in an individual elicitation interview. Interviews were divided between two interviewers, who asked interviewees the same three open-ended questions detailed in Appendix A1 to main consistency. The open-ended questions were designed to gather the participants’ thoughts, opinions, and perceptions of FUSION for undergraduate biomedical training. Participants were asked each question with intentionally varied wording multiple times to encourage deep reflection on their perspectives and experiences. Examples of how the questions were reframed are also detailed in Appendix A1. Meaningful engagement was evaluated based on list length (number of unique items provided by interviewees) and interview duration. Subsequently, participants were asked to complete a brief, anonymous demographic survey asking participants about their. The survey collected data on the following participant characteristics: race, ethnicity, gender identity, age, education level, current major, and prior research involvement.
Analysis
Following the completion of the 21 interviews, transcripts were generated and analyzed. Each transcript underwent a manual review to identify unique statements representing individual themes expressed by participants. Direct quotations from each transcript were extracted, standardized based on the recurrence and repetition of ideas [Reference Owen18], and organized into ordered lists based on the order in which the participants mentioned them [Reference Borgatti and Halgin6]. To prepare the data for the analysis, each response was assigned a unique ID, and ideas listed by each respondent were organized into lists, maintaining the order in which they were mentioned. The data was reviewed repeatedly for the consistency of wording and normalized for capitalization and punctuation. Next, Free List Analysis under R Environment using Shiny (FLARES) [Reference Wencelius, Garine and Raimond19], an open-source, cloud-based software was used to identify salient learning objectives through systematic normalization and subsequent quantitative analyses of elicited needs statements. FLARES was used to conduct quantitative analyses of the cultural salience of the unique statements identified from the interview transcripts. The following metrics were assessed: the frequency of mention (how many participants mentioned a particular item), the relative frequency of mention (the proportion of participants who mentioned the item), and Smith’s index (a measure of cultural salience calculated using both the frequency of mention and the rank order (how early on the item is listed by each participant). The Smith Index was calculated using the following formula:

where S a is the cultural salience of item a; N is number of lists (number of respondents), L i is the length of list i, and R ai is citation rank of item a in list i [Reference Smith and Borgatti8,Reference Sutrop20]. In the context of this study, cultural salience refers to the importance of the various use cases of FUSION as perceived by undergraduate students.
Following this, hierarchical cluster analysis was performed to generate a dendrogram illustrating how items were grouped into clusters.
Results
Responses from 21 participants were analyzed. Table 1 reports the participants’ demographic makeup, academic background, and previous research experience.
Table 1. Demographics, academic background, and previous research experience of the participants

1Percentages do not sum to 100 for this question since participants could select more than one response. 2Other responses were as follows: polymer synthesis (n = 1), electrical engineering sensor fusion research (n = 1), environmental science (n = 1), solid-state physics (n = 1), machine learning for electrical engineering (n = 1), and one unspecified (blank) response.
In total, 92 items were generated, of which there were 25 unique statements. The average list length, defined as the number of unique statements per individual, was 4.4. Saturation was achieved after 11 responses, at which point all 25 unique items had been cited. The data saturation plot is provided in Appendix A2. The average interview response length was 10 minutes and 52 s, the maximum was 28 minutes and 21 s, and the minimum was 6 minutes and 2 s. Table 2 reports the frequency of mention, the relative frequency of mention, and the Smith Index of the educational uses statements identified from the interview transcripts.
Table 2. Cultural saliency metrics for the perceived uses of cell visualization technology

Salience of the educational uses of technology
To address RQ1, we measured the cultural salience of the potential applications of FUSION among the undergraduate students we interviewed. Cultural salience refers to how prominent, important, or meaningful a particular item is among a specific group of individuals. An item is considered to be culturally salient if it is mentioned frequently across participants and tends to be mentioned early on by participants. Among the 25 unique statements provided by the 21 participants, the most frequently mentioned was: “analyzing cells/structures,” which was mentioned by 14 participants. Given that the data of 21 participants was analyzed, this result directly translated to a relative frequency of 0.667 for “analyzing cells/structures,” with two-thirds of participants mentioning this application. “Analyzing cells/structures” also had the highest Smith Index (0.463), indicating that this idea was the most salient within the students’ collective understanding of the potential uses of FUSION. The next most frequently cited statement was “visualizing cells/structures,” with 13 participants highlighting this use of FUSION during their interview, a relative frequency of mention of 0.619 (indicating that the idea was brought up by 61.9% of students interviewed), and a Smith Index of 0.446, indicating that this idea was also highly salient among the students interviewed. “Detecting diseases/causes” and “educating students” were also each cited multiple times, receiving 10 and 6 mentions, respectively, from the 21 participants, with relative frequencies of 0.476 and 0.286, and Smith Indices of 0.315 and 0.193, respectively. “Sharing data,” “learning about cells/structures,” and “counting cells/structures” were each mentioned 5 times, with Smith Indices of 0.139, 0.139, and 0.124, respectively. In contrast, nine statements were each mentioned only once, with the lowest Smith Index of 0.010 generated for the statement “keeping records.”
Clusters of learning objectives
Using FLARES, a dendrogram was created to illustrate the item-by-item proximity for the 25 statements (Figure 1). This dendrogram demonstrates that the 25 items, or statements, fell into eight clusters, each containing at least one consensus item. In doing so, the hierarchical relationships between the statements can be evaluated, with each merge or split representing similarity or dissimilarity between each statement, respectively.

Figure 1. Dendrogram depicting item by item proximity for the cited items.
To address RQ2, we used these clusters as a framework to formulate potential learning objectives for FUSION. Table 3 presents a list of the items contained within each cluster, along with an interpretative label for each cluster, that is, a description of the common theme linking these items together, as well as a potential learning objective based on each cluster.
Table 3. Summary of educational use clusters and sample learning objectives

Discussion
This mixed-methods study investigated the perspectives of undergraduate students regarding the educational uses of a novel AI-powered tissue visualization and analysis technology, FUSION. Through learner-centered investigation, this study provided insights about potential applications of FUSION across a variety of educational, research, and clinical settings, and applied CDA to identify and formulate learning interests and objectives for students using this technology. Our analysis of the salience of unique statements revealed that many students thought that FUSION could be used for visualizing and analyzing cells and cell structures in the traditional context of higher education courses; these being the joint most frequently mentioned potential applications of FUSION in the student interviews. Our study also generated some potential applications of the software that were less salient, with nine unique statements receiving only one mention from the students we interviewed.
The learning objectives we formulated are applicable across multiple educational settings, including research laboratories, classrooms, and clinical settings. This finding is supported by previous studies that have shown that new educational tools can be implemented across various educational, research, and clinical settings [Reference Singh, Ferry and Mills4]. As with Singh et al [Reference Singh, Ferry and Mills4]., we also found that the process of engaging students in the development of learning objectives revealed that the students we interviewed possessed an awareness of the connections between classroom-acquired knowledge and opportunities to apply that knowledge in the real world and in cross-disciplinary learning environments. For example, students recognized that FUSION could be used both for educating students in the classroom as well as in real-world applications such as medicolegal cases involving medical malpractice.
The findings presented in this study align with previous research that has advocated for learner-centered approaches in curriculum development, further emphasizing the importance of tailoring educational content to student needs [Reference Guze21–Reference O’Connor, Zhang, Trout and Snibsoer25]. Many of the learning objectives we formulated were focused on specific applications of the FUSION software, such as visualizing and analyzing cells and cell structures and identifying abnormal features of cells. We used clusters generated in the CDA as a framework for formulating specific learning objectives tailored to address these specific learning outcomes. The clustering of learning objectives reflects the clusters generated through the hierarchical clustering analysis, as conducted using our CDA methodology. Each cluster represents a set of learning objectives that share similar cognitive characteristics, aligning with the study’s aim to identify and organize meaningful groupings of learning objectives. This clustering directly supports our goal of understanding how students conceptually relate different learning objectives together, a central focus of CDA. Our methodology, therefore, provides a demonstration of how learning objectives for new educational technologies can be developed and tailored to support individuals in achieving their learning needs. Tailoring learning objectives to the student is an approach that has demonstrated success in multiple studies, giving rise to empowering learning opportunities that also encourage collaboration with others [Reference O’Connor, Zhang, Trout and Snibsoer25–Reference Zheng, Xie and Gao28].
Students also saw the versatility of FUSION use as an educational tool that could benefit learners from a spectrum of educational levels and backgrounds, ranging from elementary and high school students to medical students. Since some of the students interviewed mentioned that FUSION could be used for educating medical students, we formulated a learning objective that centered on implementing FUSION into a medical school curriculum as a tool to both educate medical students about tissues, cells, and cell structures and to deepen their understanding as to how an AI-powered software such as FUSION could be applied in their future practice as physicians. Some students also mentioned potential clinical applications of FUSION, such as to assist with communication between clinicians and patients, while another student mentioned that FUSION could be used in radiologic or ultrasound imaging; these examples further illustrate that students are cognizant of the connections between knowledge gained in the classroom and how that knowledge may be applied in the real world [Reference Singh, Ferry and Mills4]. Our findings regarding the potential applications of FUSION both as an educational tool for medical students as well as in clinical practice are supported by previous studies that have emphasized the importance of incorporating such tools into medical education, in order to equip students with the skills they will need to thrive in the technology-driven future of medicine [Reference Guze21,Reference Favero29–Reference Lee32].
Methodologically, this study demonstrates the utility of CDA in a biomedical education context, where understanding shared knowledge structures can guide the creation of learner-centered educational objectives. The high cultural salience of specific functionalities (e.g., cell analysis and visualization) reveals a clear priority for these elements in educational design, highlighting their potential to be focal points in curriculum planning and instructional materials. Additionally, usability data obtained from the free-listing of applications enhances understanding of the user experience, informing iterative software design tailored to educational needs. These insights support the co-design of training modules that are not only technically accessible but also pedagogically relevant, encouraging engagement across varied skill levels. This study thus underscores the importance of integrating student feedback into the development of educational technology, fostering not only skill acquisition but also preparing trainees for the increasingly technology-driven landscape of biomedical research and healthcare.
Limitations
This study offers valuable insights into the educational applications of cell-visualization AI technology from the student perspective; however, several limitations should be acknowledged. First, the use of a convenience sample drawn primarily from a single institution limits the generalizability of the findings. While this approach provided diverse perspectives within a controlled environment, a broader, multi-institutional sample would strengthen the validity of the results, particularly given potential differences in students’ backgrounds, prior knowledge, and exposure to similar technologies. Although the sample also included two students who had previously participated in a research experience at the Computational Microscopy Imaging Laboratory (CMIL), their limited prior exposure to the FUSION technology was unlikely to have significantly shaped their responses. Additionally, the inclusion of students with different research backgrounds was intended to enhance the generalizability of the findings.
Another important limitation was that participants were exposed to FUSION through a video demonstration rather than direct, hands-on interaction. This lack of hands-on experience likely limited their perceptions of FUSION’s usability, as their insights were shaped solely through passive observation. Additionally, while FUSION is an AI-based technology, deeper engagement with AI-specific components to make more robust claims about its educational value are needed. Finally, this study did not include a formal validation phase.
Future research and validation of learning objectives
Future studies should provide students with hands-on experience using FUSION to directly assess its usability, accessibility, and integration within real-world educational settings, thereby enhancing ecological validity (that is, the degree to which the findings generalize to real-world educational contexts). This would yield valuable insights into FUSION’s usability, accessibility, and integration within active learning settings, potentially revealing challenges and benefits not evident in passive demonstrations. In addition to incorporating hands-on interaction with FUSION, future studies should also include explicit comparisons to expert-derived learning objectives to enhance the rigor of the findings and their applicability to formal curriculum development.
Since the study did not include a formal validation phase, further assessment is required, in which we plan to engage experts in medical education, AI, and curriculum design to review and confirm that the learning objectives derived from student interviews are relevant, comprehensive, and aligned with educational goals of students. We plan to conduct further interviews with instructors, faculty, AI experts, and curriculum designers to validate our findings, and integrate their expertise with the student perspectives obtained during this study. Following this validation, we aim to pilot-test the learning objectives in a small-scale educational setting using FUSION. This will allow us to assess whether the objectives are practical, measurable, and achievable, while also collecting feedback from both students and instructors to guide further refinement.
Based on our findings and these planned steps, we propose a user-informed iterative framework to inform both curriculum development and software optimization for FUSION. This type of framework has been successfully employed in other studies evaluating how AI-based educational technologies may be used to enhance students’ educational experiences [Reference Hare, Ferguson and Tang33]. It emphasizes a continuous feedback loop, beginning with user-informed learning objectives, followed by pilot implementation, evaluation, and iterative refinement. Through collaboration between educators, students, and software developers, this approach ensures that both pedagogical strategies and technological features evolve in alignment with learner needs and curricular standards.
Conclusion
Advancements in educational technologies present both opportunities and challenges for biomedical science curriculum development. The development of learner-centered objectives can be supported by consensus-based approaches. Our findings provide valuable information into the perceptions and usability of AI-powered tissue visualization and analysis technology by revealing salient applications of such technology in the educational context among undergraduate students.
The AI-powered platform evaluated in this study, through elicitation interviews with potential users and a CDA approach, demonstrated significant potential to enhance undergraduate biomedical education. However, future research is needed, particularly that which provides students with direct hands-on experience using the technology, as well as a formal validation phase that incorporates instructor perspectives, to confirm and refine the learning outcomes developed.
Supplementary material
The supplementary material for this article can be found at https://doi.org/10.1017/cts.2025.10107
Acknowledgements
We would like to acknowledge the developers of FUSION (© 2023 University of Florida Research Foundation, Inc. All Rights Reserved), developed by the Computational Microscopy Imaging Laboratory at the University of Florida. A link to the open-source repository is provided in the References section [Reference Border34].
Author contributions
Rachel Liu-Galvin: Formal analysis, Investigation, Methodology, Resources, Software, Writing-original draft, Writing-review & editing; Nicholas Sherwin: Data curation, Validation; Selin Kavak: Data curation, Validation; Victoria Liwang: Resources; Samuel Border: Validation; Mishal Khan: Project administration; Sanjay Jain: Funding acquisition, Validation; Pinaki Sarder: Funding acquisition, Validation; Yulia Strekalova: Conceptualization, Funding acquisition, Investigation, Methodology, Supervision, Validation, Writing-review & editing.
Funding statement
Research reported in this publication was supported by the National Institutes of Health Common Fund award number 3OT2OD033753. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. The original contributions presented in the study are included in the article and supplementary material; further inquiries can be directed to the corresponding author.
Competing interests
The authors have no conflicts of interest to disclose.
Ethical standard
The study was reviewed by the University of Florida Institutional Review Board and received exempt designation (#ET00022437).