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Angiotensin I-converting enzyme inhibitory properties of whey protein digests: concentration and characterization of active peptides

Published online by Cambridge University Press:  01 February 2000

ANNE PIHLANTO-LEPPÄLÄ
Affiliation:
Agricultural Research Centre of Finland, Food Research, FIN-31600 Jokioinen, Finland
PÄIVI KOSKINEN
Affiliation:
Department of Food Technology, University of Helsinki, FIN-00014 Helsinki, Finland Present address: Valio Ltd, FIN-00039 Valio, Finland.
KATI PIILOLA
Affiliation:
Agricultural Research Centre of Finland, Food Research, FIN-31600 Jokioinen, Finland
TUOMO TUPASELA
Affiliation:
Agricultural Research Centre of Finland, Food Research, FIN-31600 Jokioinen, Finland
HANNU KORHONEN
Affiliation:
Agricultural Research Centre of Finland, Food Research, FIN-31600 Jokioinen, Finland

Abstract

The aim of this study was to identify whey-derived peptides with angiotensin I-converting enzyme (ACE) inhibitory activity. The bovine whey proteins α-lactalbumin and β-lactoglobulin were hydrolysed with pepsin, trypsin, chymotrypsin, pancreatin, elastase or carboxypeptidase alone and in combination. The total hydrolysates were fractionated in a two step ultrafiltration process, first with a 30 kDa membrane and then with a 1 kDa membrane. Inhibition of ACE was analysed spectrophotometrically. The peptides were isolated by chromatography and identified by mass and sequencing analysis. The most potent inhibitory peptides were synthesized by the 9-fluorenylmethoxycarbonyl solid phase method. Inhibition of ACE was observed after hydrolysis with trypsin alone, and with an enzyme combination containing pepsin, trypsin and chymotrypsin. Whey protein digests gave a 50 % inhibition (IC50) of ACE activity at concentration ranges within 345–1733 μg/ml. The IC50 values for the 1–30 kDa fractions ranged from 485 to 1134 μg/ml and for the <1 kDa fraction from 109 to 837 mg/ml. Several ACE-inhibitory peptides were isolated from the hydrolysates by reversed-phase chromatography, and the potencies of the purified peptide fractions had IC50 values of 77–1062 μM. The ACE-inhibitory peptides identified were α-lactalbumin fractions (50–52), (99–108) and (104–108) and β-lactoglobulin fractions (22–25), (32–40), (81–83), (94–100), (106–111) and (142–146).

Type
Research Article
Copyright
Proprietors of Journal of Dairy Research 2000

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