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Efficient milking hygiene reduces bacterial spore contamination in milk

Published online by Cambridge University Press:  23 August 2017

Luciana Bava
Affiliation:
Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, via Celoria 2, 20133Milan, Italy
Stefania Colombini
Affiliation:
Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, via Celoria 2, 20133Milan, Italy
Maddalena Zucali
Affiliation:
Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, via Celoria 2, 20133Milan, Italy
Marilù Decimo
Affiliation:
Istituto di Scienze delle Produzioni Alimentari, CNR, via Celoria 2, 20133Milan, Italy
Stefano Morandi
Affiliation:
Istituto di Scienze delle Produzioni Alimentari, CNR, via Celoria 2, 20133Milan, Italy
Tiziana Silvetti*
Affiliation:
Istituto di Scienze delle Produzioni Alimentari, CNR, via Celoria 2, 20133Milan, Italy
Milena Brasca
Affiliation:
Istituto di Scienze delle Produzioni Alimentari, CNR, via Celoria 2, 20133Milan, Italy
Alberto Tamburini
Affiliation:
Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, via Celoria 2, 20133Milan, Italy
G. Matteo Crovetto
Affiliation:
Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, via Celoria 2, 20133Milan, Italy
Anna Sandrucci
Affiliation:
Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, via Celoria 2, 20133Milan, Italy
*
*For correspondence; e-mail: tiziana.silvetti@ispa.cnr.it

Abstract

Clostridia in the milk can lead to late blowing, a cheese defect. Clostridia are ubiquitous, deriving from both the farm environment and the feed ingested by the cows, and are transferred into the milk through faecal contamination. Our aim was to investigate the effect of different in-parlour practices on the content of anaerobic spore-forming bacteria in milk, and to monitor the variation in spore content in the feed and environment. The experiment, conducted in an experimental dairy during autumn, was repeated in exactly the same way for two consecutive years. The experimental design applied three different milking routines in three consecutive 7-d periods: forestripping alone (F); forestripping and post-dipping (F+Post); pre-dipping, wiping, forestripping and post-dipping (Pre+F+Post). Teat skin swabs and samples of feed, faeces, bedding materials and milk were collected for microbiological analyses. The dietary forage of the lactating cows included maize silage, which, in both years, was found to have the highest level of clostridial spore contamination. Pre-dipping with a detergent/emollient solution, and drying with a disposable paper towel, proved much more efficient in reducing spore contamination than forestripping alone, both on the teats (1·30 vs. 2·20 log10 MPN/swab; P < 0·001) and in the milk (1·82 vs. 2·47 log10 MPN/L, P < 0·02), while post-dipping had little influence on spore count. The standard plate count in milk was significantly lower with Pre+F+Post treatment than with F (3·80 vs. 4·51 log10 CFU/mL, P < 0·01). The teat preparation procedure did not influence the lactic acid bacterial levels in the milk, which is very positive in that decreased lactic acid bacterial content can lessen raw milk cheese quality.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 2017 

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References

Al-Qumber, M & Tagg, JR 2006 Commensal bacilli inhibitory to mastitis pathogens isolated from the udder microbiota of healthy cows. Journal of Applied Microbiology 101 11521160 CrossRefGoogle ScholarPubMed
AOAC International 1995 Official Methods of Analysis, 15th edition. Washington, DC: AOAC International Google ScholarPubMed
Borreani, G & Tabacco, E 2008 Low permeability to oxygen of a new barrier film prevents butyric acid bacteria spore formation in farm corn silage. Journal of Dairy Science 91 42724281 CrossRefGoogle ScholarPubMed
Brasca, M, Bava, L, Zucali, M, Tamburini, A, Guerci, M, Sandrucci, A, Mattiello, S, Andreoli, E, Battini, M, Decimo, M, Morandi, S, Battelli, G, Povolo, M, Pelizzola, V, Passolungo, L, Sanna, M & Zanini, L 2014 Modelli di azienda zootecnica per latte e formaggi d'eccellenza. Quaderni della Ricerca n. 163 Regione Lombardia. ISBN 9788894034202Google Scholar
Colombari, G, Borreani, G & Crovetto, GM 2001 Effect of ensiling alfalfa at low and high dry matter on production of milk used to make Grana cheese. Journal of Dairy Science 84 24942502 CrossRefGoogle ScholarPubMed
Doyle, CJ, Gleeson, D, Jordan, K, Beresford, TP, Ross, RP, Fiztgerald, GF & Cotter, PD 2015 Anaerobic sporeformers and their significance with respect to milk and dairy products. International Journal of Food Microbiology 197 7787 CrossRefGoogle ScholarPubMed
Driehuis, F 2013 Silage and the safety and quality of dairy foods: a review. Agricultural and Food Science 22 1634 Google Scholar
Feligini, M, Brambati, E, Panelli, S, Ghitti, M, Sacchi, R, Capelli, E & Bonacina, C 2014 One-year investigation of Clostridium spp. occurrence in raw milk and curd of Grana Padano cheese by the automated ribosomal intergenic spacer analysis. Food Control 42 7177 CrossRefGoogle Scholar
Garde, S, Arias, R, Gaya, P & Nuñez, M 2011 Occurrence of Clostridium spp. in ovine milk and Manchego cheese with late blowing defect: identification and characterization of isolates. International Dairy Journal 21 272278 CrossRefGoogle Scholar
Gómez Torres, N, Garde, S, Peirot, A & Avila, M 2015 Impact of Clostridium spp. on cheese characteristics: microbiology, color, formation of volatile compounds and off-flavors. Food Control 56 186194 CrossRefGoogle Scholar
Magnusson, M, Christiansson, A, Svensson, B & Kolstrup, C 2006 Effect of different premilking manual teat-cleaning methods on bacterial spores in milk. Journal of Dairy Science 89 38663875 CrossRefGoogle ScholarPubMed
Mallet, A, Guéguen, M, Kauffmann, F, Chesneau, C, Sesboué, A & Desmasures, N 2012 Quantitative and qualitative microbial analysis of raw milk reveals substantial diversity influenced by herd management practices. International Dairy Journal 27 1321 CrossRefGoogle Scholar
Melin, M, Wiktorsson, H, Christiansson, A, McLean, J, Sinclair, M & West, B 2002 Teat cleaning efficiency before milking in DeLaval VMSTM vs. conventional manual cleaning, using Clostridium tyrobutyricum spores as marker. In First North American Conference on Robotic Milking, Toronto, Canada, 20–22 March 2002. Wageningen Publishers, Wageningen, the Netherlands.Google Scholar
Mertens, DR 2002 Gravimetric determination of amylase-treated neutral detergent fiber in feeds using refluxing in beakers or crucibles: collaborative study. Journal of AOAC International 85 12171240 Google ScholarPubMed
Miller, RA, Kent, DJ, Boor, KJ, Martin, NH & Wiedmann, M 2015 Different management practices are associated with the mesophilic and thermophilic spore levels in bulk tank raw milk. Journal of Dairy Science 98 43384351 CrossRefGoogle ScholarPubMed
Morandi, S, Cremonesi, P, Silvetti, T, Castiglioni, B & Brasca, M 2015 Development of a triplex real-time PCR assay for the simultaneous detection of Clostridium beijerinckii, Clostridium sporogenes and Clostridium tyrobutyricum in milk. Anaerobe 34 4449 CrossRefGoogle ScholarPubMed
Nadeau, E, Arnesson, A & Bengtsson, A 2010 Investigation of clostridial spores in Swedish dairy herds. In 14th International Symposium of Forage Conservation, Brno Czech Republic, 17–19 March 2010, pp. 79–82. http://www.cvzv.sk/icfc_web/zborniky/14th-2010.pdf Google Scholar
Pirondini, M, Malagutti, L, Colombini, S, Amodeo, A & Crovetto, GM 2012 Methane yield from dry and lactating cows diets in the Po Plain (Italy) using an in vitro gas production technique. Italian Journal of Animal Science 11(e61) 330335 CrossRefGoogle Scholar
Sandrucci, A, Bava, L, Zucali, M & Tamburini, A 2014 Management factors and cow traits influencing milk somatic cell counts and teat hyperkeratosis during different seasons. Revista Brasileira de Zootecnia 43 505511 CrossRefGoogle Scholar
SAS Institute, 2001. SAS 9.2. SAS Inst. Inc., Cary, NC, USA.Google Scholar
Schreiner, DA & Ruegg, PL 2003 Relationship between udder and leg hygiene score and subclinical mastitis. Journal of Dairy Science 86 34603465 CrossRefGoogle ScholarPubMed
Tormo, H, Lekhal, DAH & Roques, C 2015 Phenotypic and genotypic characterization of lactic acid bacteria isolated from raw goat milk and effect of farming practices on the dominant species of lactic acid bacteria. International Journal of Food Microbiology 210 915 CrossRefGoogle ScholarPubMed
Vacheyrou, M, Normand, AC, Guyot, P, Cassagne, C, Piarroux, R & Bouton, Y 2011 Cultivable microbial communities in raw cow milk and potential transfers from stables of sixteen French farms. International Journal of Food Microbiology 146 253262 CrossRefGoogle ScholarPubMed
Van Soest, PJ, Robertson, JB & Lewis, BA 1991 Methods ofdietary fiber, neutral detergent fiber and non-polysaccharides in relation to animal nutrition. Journal of Dairy Science 74 35833597 CrossRefGoogle Scholar
Vissers, MMM, Driehuis, F, Te Giffel, MC, De Jong, P & Lankveld, JMG 2007a Short communication: quantification of the transmission of microorganisms to milk via dirt attached to the exterior of teats. Journal of Dairy Science 90 35793582 CrossRefGoogle ScholarPubMed
Vissers, MMM, Driehuis, F, Te Giffel, MC, De Jong, P & Lankveld, JMG 2007b Concentrations of butyric acid bacteria spores in silage and relationships with aerobic deterioration. Journal of Dairy Science 90 928936 CrossRefGoogle ScholarPubMed
Zucali, M, Bava, L, Tamburini, A, Brasca, M, Vanoni, L & Sandrucci, A 2011 Effects of season, milking routine and cow cleanliness on bacterial and somatic cell counts of bulk tank milk. Journal of Dairy Research 78 436441 CrossRefGoogle ScholarPubMed
Zucali, M, Bava, L, Colombini, S, Brasca, M, Decimo, M, Morandi, S, Tamburini, A & Crovetto, GM 2015 Management practices and forage quality affecting the contamination of milk with anaerobic spore-forming bacteria. Journal of the Science of Food and Agriculture 95 12941302 CrossRefGoogle ScholarPubMed
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