Hostname: page-component-7dd5485656-wjfd9 Total loading time: 0 Render date: 2025-10-29T06:17:04.273Z Has data issue: false hasContentIssue false
  • English
  • Français

Rapid and sensitive immunomagnetic separation–polymerase chain reaction method for the detection of Escherichia coli O157[ratio ]H7 in raw milk and ice-cream

Published online by Cambridge University Press:  01 February 1997

CHRISTOPHER M. GOODING  and
PRABHAKARA V. CHOUDARY
CHRISTOPHER M. GOODING
Affiliation:
CIFAR, Antibody Engineering Laboratory and Department of Entomology, University of California, Davis, CA 95616-8584, USA
PRABHAKARA V. CHOUDARY
Affiliation:
CIFAR, Antibody Engineering Laboratory and Department of Entomology, University of California, Davis, CA 95616-8584, USA
Get access Rights & Permissions [Opens in a new window]

Abstract

Escherichia coli O157[ratio ]H7 in spiked samples of raw milk and ice-cream was enriched in tryptic soy broth for 4 h, captured by immunomagnetic separation, subjected to amplification by polymerase chain reaction of parts of the verotoxin genes (SLT-I and SLT-II), and detected by agarose gel electrophoresis. Using this method, as few as 1 cfu Esch. coli O157[ratio ]H7/g food could be detected in <10 h.

Information

Type
Research Article
Information
Journal of Dairy Research , Volume 64 , Issue 1 , February 1997 , pp. 87 - 93
Copyright
Proprietors of Journal of Dairy Research 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable