Skip to main content Accessibility help
×
Home
Hostname: page-component-5f95dd588d-b59hz Total loading time: 0.151 Render date: 2021-10-28T12:30:32.850Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }

Rapid detection of cow milk adulteration/contamination in goat milk by a lateral flow colloidal gold immunoassay strip

Published online by Cambridge University Press:  07 February 2019

Bochao Liu
Affiliation:
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China
Jinhong Si
Affiliation:
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China
Fang Zhao
Affiliation:
Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety, Technical Centre for Food Inspection and Quarantine, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China Shenzhen Academy of Inspection and Quarantine, Shenzhen, China
Qi Wang
Affiliation:
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China
Yu Wang
Affiliation:
Nanjing Entry-exit Inspection and Quarantine Bureau, Nanjing, China
Jinfeng Li
Affiliation:
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China
Chengyao Li
Affiliation:
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China
Tingting Li*
Affiliation:
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China Shenzhen Key Research Laboratory of Detection Technology R&D on Food Safety, Technical Centre for Food Inspection and Quarantine, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, China Shenzhen Academy of Inspection and Quarantine, Shenzhen, China Nanjing Entry-exit Inspection and Quarantine Bureau, Nanjing, China School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China
*
Author for correspondence: Tingting Li, Email: apple-ting-007@163.com

Abstract

Current available methods to detect cow milk adulteration or accidental contamination of goat milk are both laborious and time consuming. The aim of this technical research communication was to develop a simple, rapid, specific and sensitive method for quantitative detection of cow milk in goat milk. A competitive lateral flow immunoassay (LFIA) strip was developed using a specific monoclonal antibody (mAb) labeled with colloidal gold nanoparticles (GNPs) for specifically binding to cow milk casein. The detection limit of this rapid detection was 0.07% of cow milk in goat milk, providing equal specificity and higher sensitivity when compared with a commercial enzyme-linked immunosorbent assay (ELISA). These result suggest that the established rapid GNPs-LFIA strip could be used for monitoring cow milk adulteration/contamination of goat milk.

Type
Research Article
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

These authors contributed equally to this work.

References

Agamy, EI (2007) The challenge of cow milk protein allergy. Small Ruminant Research 68, 6472.CrossRefGoogle Scholar
Alférez, MJ, Rivas, E, Díaz-Castro, J, Hijano, S, Nestares, T, Moreno, M, Campos, MS, Serrano-Reina, JA and López-Aliaga, I (2015) Folic acid supplemented goat milk has beneficial effects on hepatic physiology, haematological status and antioxidant defence during chronic Fe repletion. Journal of Dairy Research 82, 8694.CrossRefGoogle ScholarPubMed
Ballabio, C, Chessa, S, Rignanese, D, Gigliotti, C, Pagnacco, G and Terracciano, L (2011) Goat milk allergenicity as a function of alphas(1)-casein genetic polymorphism. Journal of Dairy Science 94, 9981004.CrossRefGoogle ScholarPubMed
Claeys, WL, Cardoen, S, Daube, G, De Block, J, Dewettinck, K and Dierick, K (2013) Raw or heated cow milk consumption: review of risks and benefits. Food Control 31, 251262.CrossRefGoogle Scholar
Haenlein, GFW (2004) Goat milk in human nutrition. Small Ruminant Research 511, 55163.Google Scholar
Ke, X, Zhang, J, Lai, S, Chen, Q, Zhang, Y, Jiang, Y, Mo, W and Ren, Y (2017) Quantitative analysis of cow whole milk and whey powder adulteration percentage in goat and sheep milk products by isotopic dilution-ultra-high performance liquid chromatography-tandem mass spectrometry. Analytical and Bioanalytical Chemistry 409, 213224.CrossRefGoogle ScholarPubMed
Li, J, Hu, F, Chen, S, Luo, P, He, Z, Wang, W, Allain, JP and Li, C (2017) Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies. BMC Microbiology 17, 115.CrossRefGoogle ScholarPubMed
Moreno-Fernández, J, Díaz-Castro, J, Alférez, MJ, Hijano, S, Nestares, T and López-Aliaga, I (2016) Production and chemical composition of two dehydrated fermented dairy products based on cow or goat milk. Journal of Dairy Research 83, 8188.CrossRefGoogle ScholarPubMed
Qiu, J, Wang, W, Wu, J, Zhang, H, Wang, Y, Qiao, J, Chen, C, Gao, GF, Allain, JP and Li, C (2012) Characterization of periplasmic protein BP26 epitopes of Brucella melitensis reacting with murine monoclonal and sheep antibodies. PLoS One 7(3), e34246.CrossRefGoogle ScholarPubMed
Yokota, S (1992) Preparation of colloidal gold probes. In Fujimori, O (Eds), Methods of Immunogold Staining. Soft Science Publications: Japan, pp. 8288.Google Scholar
Zhang, G, Wang, X, Yang, J, Yang, Y, Xing, G, Li, Q, Zhao, D, Chai, S and Guo, J (2006) Development of an immunochromatographic lateral flow test strip for detection of -adrenergic agonist clenbuterol residues. Journal of Immunology Methods 312, 2733.CrossRefGoogle ScholarPubMed
Zhang, G, Wang, X, Zhi, A, Bao, Y, Yang, Y, Qu, M, Luo, J, Li, Q, Guo, J, Wang, Z, Yang, J, Xing, G, Chai, S, Shi, T and Liu, Q (2008) Development of a lateral flow immunoassay strip for screening of sulfamonomethoxine residues. Food Additives and Contaminants 25, 413423.CrossRefGoogle ScholarPubMed
6
Cited by

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Rapid detection of cow milk adulteration/contamination in goat milk by a lateral flow colloidal gold immunoassay strip
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Rapid detection of cow milk adulteration/contamination in goat milk by a lateral flow colloidal gold immunoassay strip
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Rapid detection of cow milk adulteration/contamination in goat milk by a lateral flow colloidal gold immunoassay strip
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *