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The reaction of lactose with anthrone and its application to the estimation of lactose in casein and other dairy products

Published online by Cambridge University Press:  01 June 2009

E. L. Richards
E. L. Richards
Affiliation:
The Dairy Research Institute (N.Z.), Palmerston North, New Zeland
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Extract

Lactose was estimated accurately and rapidly in casein by use of anthrone reagent on the clear filtrate from precipitation of a sodium bicarbonate solution of the casein with 0·1 n-sulphuric acid. The reagent consists of 0·15% (w/v) anthrone in 70% (v/v) sulphuric acid. The ice-cold anthrone solution (10 ml.) was added, with cooling, to 1 ml. of the ice-cold solution containing 0–100 µg. of lactose. The mixture was heated at 100° C. for 6 min. and cooled for 30 min. The optical density of the coloured solution was then measured at 625 mµ

Information

Type
Original Articles
Information
Journal of Dairy Research , Volume 26 , Issue 1 , February 1959 , pp. 53 - 57
Copyright
Copyright © Proprietors of Journal of Dairy Research 1959

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References

REFERENCES

(1)Dreywood, R. (1946). lndustr. Engng Chem. (Anal.), 18, 499.CrossRefGoogle Scholar
(2)Bailey, R. W. (1958). Biochem. J. 68, 669.CrossRefGoogle Scholar
(3)McCready, R. M., Guggolz, J., Silviera, V. & Owens, H. S. (1950). Anal. Chem. 22, 1156.CrossRefGoogle Scholar
(4)Black, H. C. (1951). Anal. Chem. 23, 1792.CrossRefGoogle Scholar
(5)Fagen, H. J., Sibbach, E. & Hussong, R. V. (1954). J. Dairy Sci. 37, 10.CrossRefGoogle Scholar
(6)British Standards Institution. B.S. 1417: 1948.Google Scholar
(7)Spellacy, J. R. (1953). Casein Dried and Condensed Whey, 1st ed. p. 117. San Francisco: Lithotype Process Co.Google Scholar
(8)Sattler, L. & Zerban, F. W. (1948). Science, 108, 207.CrossRefGoogle Scholar