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Acceleration of yoghurt fermentation time by yeast extract and partial characterisation of the active components

Published online by Cambridge University Press:  29 October 2014

Esti-Andrine Smith
Affiliation:
Department of Microbial, Biochemical and Food Biotechnology, Faculty of Natural and Agricultural Science, University of the Free State, ✉ 339 Bloemfontein, 9300, Republic of South Africa
Jacobus Myburgh*
Affiliation:
Department of Microbial, Biochemical and Food Biotechnology, Faculty of Natural and Agricultural Science, University of the Free State, ✉ 339 Bloemfontein, 9300, Republic of South Africa
Gernot Osthoff
Affiliation:
Department of Microbial, Biochemical and Food Biotechnology, Faculty of Natural and Agricultural Science, University of the Free State, ✉ 339 Bloemfontein, 9300, Republic of South Africa
Maryna de Wit
Affiliation:
Department of Microbial, Biochemical and Food Biotechnology, Faculty of Natural and Agricultural Science, University of the Free State, ✉ 339 Bloemfontein, 9300, Republic of South Africa
*
*For correspondence; e-mail: myburghj@ufs.ac.za

Abstract

Water soluble autolysate of yeast, usually utilised for microbial growth support, was used as additive in yoghurt fermentation. The yeast extract (YE) resulted in a decrease of fermentation time by 21% to reach a pH of 4·6. However, the YE resulted in unacceptable flavour and taste. By size exclusion chromatography, a fraction of the YE was obtained that could account for the observed 21% decrease in fermentation time. The fraction contained molecules of low molecular weight, consisting of minerals, free amino acids and peptides. The acceleration of the yoghurt fermentation was ascribed to the short peptides in the fraction. It is proposed that the application of this extract in industrial yoghurt manufacture would result in savings for both the industry and the consumer.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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References

Amrane, A & Prigent, Y 1998 Influence of YE concentration on batch cultures of Lactobacillus helveticus: growth and production coupling. World Journal of Microbiology and Biotechnology 14 529534 Google Scholar
Arkbage, K 2003. Vitamin B12, Folate and Folate-binding Proteins in Dairy Products. Doctoral Thesis. Swedish University of Agricultural Sciences: Sweden Google Scholar
Barrette, J, Champagne, CP & Goulet, J 2001 Growth-promoting properties of YEs produced at different pH values, with different autolysis promoters and bacterial populations. Journal of Chemical Technology and Biotechnology 76 203209 Google Scholar
Chae, HJ, Joo, H & In, M 2001 Utilization of brewer's yeast cells for the production of food-grade YE Part 1: effects of different enzymatic treatments on solid and protein recovery and flavor characteristics. Bioresource Technology 76 253258 Google Scholar
Champagne, C, Gaudreau, H & Conway, J 2003 Effect of the production or use of mixtures of bakers’ or brewers' YEs on their ability to promote growth of lactobacilli and pediococci. Electronic Journal of Biotechnology 6 185197 Google Scholar
de Lima, CJB, Coelho, LF & Contiero, J 2010 The use of response surface methodology in optimization of lactic acid production: Focus on medium supplementation, temperature and pH control. Food Technology and Biotechnology 48 175181 Google Scholar
du Preez, JC & van der Walt, JP 1983 Fermentation of D-xylose to ethanol by a strain of Candida shehatae . Biotechnology Letters 5 357362 Google Scholar
Elli, M, Zink, R, Reniero, R & Morelli, L 1999 Growth requirements of Lactobacillus johnsonii in skim and UHT milk. International Dairy Journal 9 507513 Google Scholar
Folkertsma, B & Fox, PF 1992 Use of the Cd-ninhydrin reagent to assess proteolysis in cheese during ripening. Journal of Dairy Research 59 217224 Google Scholar
Ghaly, AE, Tango, MSA & Adams, MA 2003 Enhanced lactic acid production from cheese whey with nutrient supplement addition. Agricultural Engineering International: the CIGR Journal of Scientific Research and Development 5 120 Google Scholar
Iyer, R, Tomar, SK, Maheswari, T & Singh, R 2010 Streptococcus thermophilus strains: Multifunctional lactic acid bacteria. International Dairy Journal 20 133141 Google Scholar
Letort, C & Juillard, V 2001 Development of a minimal chemically-defined medium for the exponential growth of Streptococcus thermophilus . Journal of Applied Microbiology 96 10231029 Google Scholar
Robinson, RK 2001 Dairy Microbiology Handbook, pp. 138 New York: Wiley Interscience Google Scholar
Shihata, A 2004 The proteolytic activity of yoghurt and probiotic bacteria for the improved viability of probiotic bacteria in fermented milk products. Doctoral Thesis. Australia: Victoria University Google Scholar
Smith, JS, Hillier, AJ & Lees, GJ 1975 The nature of the stimulation of the growth of Streptococcus lactis by YE. Journal of Dairy Research 42 123138 Google Scholar
Tamime, AY & Robinson, RK 1996 Background to manufacturing practice. In Yoghurt: Science and Technology, pp. 647 Cambridge, UK: Woodhead Publishing Google Scholar
Watanabe, M & Arai, S 1992 The plastein reaction: fundamentals and applications. In Biochemistry of Food Proteins, pp. 271305 (Ed. Hudson, BJF). Bath, UK: Springer Google Scholar
Yoo, SM 2009 Fractionation of non-animal protein hydrolysates for use in CHO (Chinese Hamster Ovary) cell media. Masters Thesis. Canada: University of Waterloo Google Scholar