Skip to main content Accessibility help

Analysis of microsatellite markers in a Cuban water buffalo breed

  • Odalys Uffo (a1), Nadia Martínez (a1), Atzel Acosta (a1), Arianne Sanz (a2), Inmaculada Martín-Burriel (a2), Rosario Osta (a2), Clementina Rodellar (a2) and Pilar Zaragoza (a2)...


The aim of this Regional Research Communication was to validate a panel of 30 microsatellite markers recommended by FAO/ISAG for studies of biodiversity in cattle to improve the characterisation of Cuban buffalo populations. The water buffalo (Bubalus bubalis) is an economically important livestock species. Therefore, research focused on the study of the genetic relationships among water buffalo populations is useful to support conservation decisions and to design breeding schemes. Twenty-eight of the 30 tested regions were amplified, one of which (ETH10) turned out to be monomorphic. A total of 143 alleles were observed in the Cuban water buffalo population. The average number of alleles per locus was 5·04. The number of alleles per polymorphic locus ranged from two (INRA 63 and MM12) to nine (ETH185). The observed and expected heterozygosity ranged from 0·108 (HAUT24) to 0·851 (CSSM66) and 0·104 (MM12) to 0·829(INRA32), respectively. The polymorphic information content (PIC) ranged from 0·097 (MM12) to 0·806 (INRA32), and the overall value for these markers was 0·482. Within the population, inbreeding estimates (F IS) was positive in 14 of the 30 loci analysed. This study thus highlights the usefulness of heterologous bovine microsatellite markers to assess the genetic variability in Cuban water buffalo breeds. Furthermore, the results can be utilised for future breeding strategies and conservation.


Corresponding author

*For correspondence; e-mail:


Hide All
Abou-Bakr, S, Ibrahim, MAM, Hafez, YM, Attia, M, Abdel-Salam, SM & Mekkawy, W 2012 Genetic characteristics of Egyptian buffalo using DNA microsatellite markers. Egyptian Journal of Animal Production 49 121126
Acosta, A, Uffo, O, Sanz, A, Obregón, D, Ronda, R, Osta, R, Martin-Burriel, I, Rodellar, C & Zaragoza, P 2014 Genetic characterization of the Cuban water buffalo population using microsatellite DNA markers. Buffalo Bulletin 33 101106
Attia, M, Abou-Bakr, S & Nigm, A 2014 Genetic differentiation and relationship among Egyptian Nile Delta located buffalo using microsatellite markers. Egyptian Journal of Animal Production 51 7177
Borghese, A & Mazzi, M 2005 Buffalo population and strategies in the world. In Buffalo Production and Research, pp. 140 (Ed. Borghese, A). Rome: FAO, Monterotondo, Roma, Italia
FAO 2011 Molecular genetic characterization of animal genetic resources. FAO Animal Production and Health Guidelines. No. 9. Rome
Freeman, AR, Bradley, DG, Nagda, S, Gibson, JP & Hanotte, O 2005 Combination of multiple microsatellite data sets to investigate genetic diversity and admixture of domestic cattle. Animal Genetics 37 19
Martínez, N, da Silva, EC, Mitat, A, Ponce, G, Benício da Silva, RC, Paes Barbosa, SB, Uffo, O & Gomes Filho, MA 2015 Genetic relationships between Cuban and Brazilian buffaloes (Bubalus bubalis) by microsatellite markers. Revista Salud Animal 37 152163
Michelizzi, VN, Dodson, MV, Pan, Z, Amaral, MEJ, Michal, JJ, McLean, DJ, Womack, JE & Jiang, Z 2010 Water buffalo genome science comes of age. International Journal of Biology Science 6 333349
Mitat, A 2009 Búfalos de agua en Cuba. Origen y evolución. Revista ACPA 3 4548
Nagarajan, M, Kumar, N, Nishanth, G, Haribaskar, R, Paranthaman, K, Gupta, J, Mishra, M, Vaidhegi, R, Kumar, S, Rajan, AK & Kumar, S 2009 Microsatellites markers of water buffalo, Bubalus bubalis – development, characterization and linkage disequilibrium studies. BMC Genetics 10 68
Santana, ML, Aspilcueta-Borquis, RR, Bignardi, AB, Albuquerque, LG & Tonhati, H 2011 Population structure and effects of inbreeding on milk yield and quality of Murrah buffaloes. Journal of Dairy Science 94 52045211
Shokrollahi, B, Amirinia, C, Djadid, ND, Amirmozaffari, N & Ali Kamali, M 2009 Development of polymorphic microsatellite loci for Iranian river buffalo (Bubalus bubalis). African Journal of Biotechnology 8 67506755
Vieira, JN, Teixeira, SC, Kuabara, MY & Andrade de Oliveira, DA 2011 DNA microsatellites for genetic identification in Brazilian Murrah water buffaloes. Acta Veterinaria Brasilica 5 364367


Type Description Title
Supplementary materials

Uffo et al supplementary material
Uffo et al supplementary material

 PDF (355 KB)
355 KB


Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed