Hostname: page-component-8448b6f56d-qsmjn Total loading time: 0 Render date: 2024-04-19T02:19:59.478Z Has data issue: false hasContentIssue false
  • English
  • Français

Classification of newly calved cows into moderate and severe responders to experimentally induced Escherichia coli mastitis

Published online by Cambridge University Press:  01 June 2009

Georgette Vandeputte-Van Messom ,
Christian Burvenich ,
Eddy Roets ,
Anne-Marie Massart-Leën ,
Roger Heyneman ,
Wim D. J. Kremer  and
Arie Brand
Georgette Vandeputte-Van Messom
Affiliation:
Departments of Veterinary Physiology, University of Ghent, Casinoplein 24, B-9000 Gent, Belgium
Christian Burvenich
Affiliation:
Departments of Veterinary Physiology, University of Ghent, Casinoplein 24, B-9000 Gent, Belgium
Eddy Roets
Affiliation:
Departments of Veterinary Physiology, University of Ghent, Casinoplein 24, B-9000 Gent, Belgium
Anne-Marie Massart-Leën
Affiliation:
Departments of Veterinary Physiology, University of Ghent, Casinoplein 24, B-9000 Gent, Belgium
Roger Heyneman
Affiliation:
Departments of Physiological Chemistry, University of Ghent, Casinoplein 24, B-9000 Gent, Belgium
Wim D. J. Kremer
Affiliation:
Department of Herd Health and Reproduction, Yalelaan 7, 3584 CL Utrecht, The Netherlands
Arie Brand
Affiliation:
Department of Herd Health and Reproduction, Yalelaan 7, 3584 CL Utrecht, The Netherlands
Get access Rights & Permissions [Opens in a new window]

Summary

In the present study newly calved cows were tentatively classified as moderate and severe responders to experimentally induced Escherichia coli mastitis based upon the reactive oxygen species (ROS)-generating capacity of their blood neutrophils before infection. The groups differed in blood and milk composition prior to infection. This initial classification was supported by the corresponding variation in clinical symptoms and in the changes in milk production and composition measured during mastitis. Responses of newly calved cows to Esch. coli challenge varied from mild to severe symptoms of inflammation in infected glands and differed in the intensity of systemic disturbances and general illness. Losses in milk yield and compositional changes were most pronounced in inflamed glands and in severe responders. In inflamed glands milk yield and composition did not return to preinfection level in either moderate or severe responders. The yields of lactose, α-lactalbumin, casein and fat followed the same pattern as milk yield. It is concluded that the severe and long lasting systemic disturbances observed in severe responders can be ascribed to absorption of endotoxin from infected glands into circulation, indicating the important role of endotoxin in the pathology of coliform mastitis in periparturient cows. Evaluation of the ROS-generating capacity of blood neutrophils and blood and milk composition before infection might help to predict the cow's sensitivity to Esch. coli mastitis.

Type
Original Articles
Information
Journal of Dairy Research , Volume 60 , Issue 1 , February 1993 , pp. 19 - 29
Copyright
Copyright © Proprietors of Journal of Dairy Research 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Beck, N. F. G. & Tucker, H. A. 1977 Relationships between radioimmunoassays of alpha lactalbumin and prolactin in bovine skim milk. Journal of Dairy Science 60 542–545CrossRefGoogle ScholarPubMed
Bellavite, P. 1988 The Superoxide forming enzymatic system of phagocytes. Free Radical Biology and Medicine 4 221–261CrossRefGoogle ScholarPubMed
Bramley, A. J. 1976 Variations in the susceptibility of lactating and non-lactating bovine udders to infection when infused with Escherichia coli. Journal of Dairy Research 43 205–211CrossRefGoogle ScholarPubMed
Burvenich, C., Heyneman, R., Fabry, J., Vandeputte-Van Messom, G., Massart-Leen, A. M. & Roets, E. 1989 Differences in local and systemic reactions during severe and moderate cases of E. coli mastitis in cattle soon after parturition. In Proceedings of the International Conference of Mastitis, pp. 20–25, Kärnten, AustriaGoogle Scholar
Burvenich, C., Reynaert, R., Vandeputte-Van Messom, G. & Peeters, G. 1985 Plasma somatotropin in lactating goats after intramammary and intravenous endotoxin administration. Archives Internationales de Physiologie et de Biochimie 93 P25–P27Google Scholar
Burvenich, C., Vandeputte-Van Messom, G., Heyneman, R., Massart-Leen, A. M., Roets, E. & Fabry, J. 1990 The mammary gland as model for the study of local and systemic inflammatory reactions. In Veterinary Pharmacology, Toxicology and Therapy in Food Producing Animals, pp. 213–222 (Eds Simon, P., Lees, P. and Semjén, G.). Budapest, Hungary: University of Veterinary ScienceGoogle Scholar
Curtis, C. R., Erb, H. N., Sniffen, C. J., Smith, R. D., Powers, P. A., Smith, M. C., White, M. E., Hillman, R. B. & Pearson, E. J. 1983 Association of parturient hypocalcemia with eight periparturient disorders in Holstein cows. Journal of the American Veterinary Medical Association 183 559–561Google ScholarPubMed
Fox, L. K., Heald, C. W., Gwazdauskas, F. C. & Vinson, W. E. 1981 Concentrations of glucocorticoids, bovine serum albumin, and somatic cells in mastitic milk. Journal of Dairy Science 64 2258–2261CrossRefGoogle ScholarPubMed
Guzmán, A. A., Pérez, M. D., Marquez, R. M. & Garza, J. R. 1986 Colorimetric determination of bovine serum albumin (BSA) in milk using bromocresol green dye. Journal of Dairy Science 69 (Suppl. 1) 102 (Abstr.)Google Scholar
Harmon, R. J. & Heald, C. W. 1979 Neutrophil migration in the udder during mastitis. In Proceedings of the 18th Annual Meeting of the National Mastitis Council, pp. 25–31. Washington, DC: National Mastitis CouncilGoogle Scholar
Heyneman, R., Burvenich, C. & Vercauteren, R. 1990 Interaction between the respiratory burst activity of neutrophil leukocytes and experimentally induced Escherichia coli mastitis in cows. Journal of Dairy Science 73 985–994CrossRefGoogle ScholarPubMed
Hill, A. W., Shears, A. L. & Hibbitt, K. G. 1978 The elimination of serum-resistant Escherichia coli from experimentally infected single mammary glands of healthy cows. Research in Veterinary Science 25 89–93CrossRefGoogle ScholarPubMed
Hill, A. W., Shears, A. L. & Hibbitt, K. G. 1979 The pathogenesis of experimental Escherichia coli mastitis in newly calved dairy cows. Research in Veterinary Science 26 97–101CrossRefGoogle ScholarPubMed
McDermott, C. M., Morrill, J. L. & Fenwick, B. W. 1991 Deacylation of endotoxin during natural cases of bovine mastitis. Journal of Dairy Science 74 1227–1234CrossRefGoogle ScholarPubMed
McFadden, T. B., Akers, R. M. & Kazmer, G. W. 1987 Alpha-lactalbumin in bovine serum: relationships with udder development and function. Journal of Dairy Science 70 259–264CrossRefGoogle Scholar
Nelson, N. 1944 A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153 375–380CrossRefGoogle Scholar
Rainard, P. 1985 Assessment of attachment, ingestion, and killing of Escherichia, coli by bovine polymorphonuclear cells with combined micromethods. Veterinary Immunology and Immunopathology 10 155–165CrossRefGoogle ScholarPubMed
Schalm, O. W., Lasmanis, J. & Jain, N. C. 1967 Effects of humoral and cellular phases of acute inflammation in the bovine mammary gland on Aerobacter aerogenes introduced en masse and on leukocytes existing in milk. American Journal of Veterinary Research 28 1251–1256Google ScholarPubMed
Snedecor, G. W. & Cochran, W. G. 1967 Statistical Methods, 6th edn.Ames, IA: Iowa State University PressGoogle Scholar
Vandeputte-Van Messom, G. & Burvenich, C. 1989 Comparison of fat and cream content in normal and mastitis milk of cows. Veterinary Quarterly 11 61–64CrossRefGoogle ScholarPubMed
Vandeputte-Van Messom, G., Burvenich, C., Roets, E. & Devriese, L. A. 1988 Effect of bovine somatotropin on milk yield and composition during Escherichia coli induced mastitis in lactating cows: some preliminary results. Vlaams Diergeneeskundig Tijdschrift 57 53–61Google Scholar
Vandeputte-Van Messom, G., Massart-Leen, A. M. & Burvenich, C. 1989 Endocrinological changes in newty calved cows during experimentally induced E. coli mastitis. In Proceedings of the 7th International Conference on Production Diseases in Farm Animals, pp. 346–349. Ithaca, NY: Cornell UniversityGoogle Scholar
Wheelock, J. V., Rook, J. A. F., Neave, F. K. & Dodd, F. H. 1966 The effect of bacterial infections of the udder on the yield and composition of cow's milk. Journal of Dairy Research 33 199–215CrossRefGoogle Scholar