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Classification of newly calved cows into moderate and severe responders to experimentally induced Escherichia coli mastitis

  • Georgette Vandeputte-Van Messom (a1), Christian Burvenich (a1), Eddy Roets (a1), Anne-Marie Massart-Leën (a1), Roger Heyneman (a2), Wim D. J. Kremer (a3) and Arie Brand (a3)...

In the present study newly calved cows were tentatively classified as moderate and severe responders to experimentally induced Escherichia coli mastitis based upon the reactive oxygen species (ROS)-generating capacity of their blood neutrophils before infection. The groups differed in blood and milk composition prior to infection. This initial classification was supported by the corresponding variation in clinical symptoms and in the changes in milk production and composition measured during mastitis. Responses of newly calved cows to Esch. coli challenge varied from mild to severe symptoms of inflammation in infected glands and differed in the intensity of systemic disturbances and general illness. Losses in milk yield and compositional changes were most pronounced in inflamed glands and in severe responders. In inflamed glands milk yield and composition did not return to preinfection level in either moderate or severe responders. The yields of lactose, α-lactalbumin, casein and fat followed the same pattern as milk yield. It is concluded that the severe and long lasting systemic disturbances observed in severe responders can be ascribed to absorption of endotoxin from infected glands into circulation, indicating the important role of endotoxin in the pathology of coliform mastitis in periparturient cows. Evaluation of the ROS-generating capacity of blood neutrophils and blood and milk composition before infection might help to predict the cow's sensitivity to Esch. coli mastitis.

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Beck N. F. G. & Tucker H. A. 1977 Relationships between radioimmunoassays of alpha lactalbumin and prolactin in bovine skim milk. Journal of Dairy Science 60 542545
Bellavite P. 1988 The Superoxide forming enzymatic system of phagocytes. Free Radical Biology and Medicine 4 221261
Bramley A. J. 1976 Variations in the susceptibility of lactating and non-lactating bovine udders to infection when infused with Escherichia coli. Journal of Dairy Research 43 205211
Burvenich C., Heyneman R., Fabry J., Vandeputte-Van Messom G., Massart-Leen A. M. & Roets E. 1989 Differences in local and systemic reactions during severe and moderate cases of E. coli mastitis in cattle soon after parturition. In Proceedings of the International Conference of Mastitis, pp. 2025, Kärnten, Austria
Burvenich C., Reynaert R., Vandeputte-Van Messom G. & Peeters G. 1985 Plasma somatotropin in lactating goats after intramammary and intravenous endotoxin administration. Archives Internationales de Physiologie et de Biochimie 93 P25–P27
Burvenich C., Vandeputte-Van Messom G., Heyneman R., Massart-Leen A. M., Roets E. & Fabry J. 1990 The mammary gland as model for the study of local and systemic inflammatory reactions. In Veterinary Pharmacology, Toxicology and Therapy in Food Producing Animals, pp. 213222 (Eds Simon P., Lees P. and Semjén G.). Budapest, Hungary: University of Veterinary Science
Curtis C. R., Erb H. N., Sniffen C. J., Smith R. D., Powers P. A., Smith M. C., White M. E., Hillman R. B. & Pearson E. J. 1983 Association of parturient hypocalcemia with eight periparturient disorders in Holstein cows. Journal of the American Veterinary Medical Association 183 559561
Fox L. K., Heald C. W., Gwazdauskas F. C. & Vinson W. E. 1981 Concentrations of glucocorticoids, bovine serum albumin, and somatic cells in mastitic milk. Journal of Dairy Science 64 22582261
Guzmán A. A., Pérez M. D., Marquez R. M. & Garza J. R. 1986 Colorimetric determination of bovine serum albumin (BSA) in milk using bromocresol green dye. Journal of Dairy Science 69 (Suppl. 1) 102 (Abstr.)
Harmon R. J. & Heald C. W. 1979 Neutrophil migration in the udder during mastitis. In Proceedings of the 18th Annual Meeting of the National Mastitis Council, pp. 2531. Washington, DC: National Mastitis Council
Heyneman R., Burvenich C. & Vercauteren R. 1990 Interaction between the respiratory burst activity of neutrophil leukocytes and experimentally induced Escherichia coli mastitis in cows. Journal of Dairy Science 73 985994
Hill A. W., Shears A. L. & Hibbitt K. G. 1978 The elimination of serum-resistant Escherichia coli from experimentally infected single mammary glands of healthy cows. Research in Veterinary Science 25 8993
Hill A. W., Shears A. L. & Hibbitt K. G. 1979 The pathogenesis of experimental Escherichia coli mastitis in newly calved dairy cows. Research in Veterinary Science 26 97101
McDermott C. M., Morrill J. L. & Fenwick B. W. 1991 Deacylation of endotoxin during natural cases of bovine mastitis. Journal of Dairy Science 74 12271234
McFadden T. B., Akers R. M. & Kazmer G. W. 1987 Alpha-lactalbumin in bovine serum: relationships with udder development and function. Journal of Dairy Science 70 259264
Nelson N. 1944 A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153 375380
Rainard P. 1985 Assessment of attachment, ingestion, and killing of Escherichia, coli by bovine polymorphonuclear cells with combined micromethods. Veterinary Immunology and Immunopathology 10 155165
Schalm O. W., Lasmanis J. & Jain N. C. 1967 Effects of humoral and cellular phases of acute inflammation in the bovine mammary gland on Aerobacter aerogenes introduced en masse and on leukocytes existing in milk. American Journal of Veterinary Research 28 12511256
Snedecor G. W. & Cochran W. G. 1967 Statistical Methods, 6th edn.Ames, IA: Iowa State University Press
Vandeputte-Van Messom G. & Burvenich C. 1989 Comparison of fat and cream content in normal and mastitis milk of cows. Veterinary Quarterly 11 6164
Vandeputte-Van Messom G., Burvenich C., Roets E. & Devriese L. A. 1988 Effect of bovine somatotropin on milk yield and composition during Escherichia coli induced mastitis in lactating cows: some preliminary results. Vlaams Diergeneeskundig Tijdschrift 57 5361
Vandeputte-Van Messom G., Massart-Leen A. M. & Burvenich C. 1989 Endocrinological changes in newty calved cows during experimentally induced E. coli mastitis. In Proceedings of the 7th International Conference on Production Diseases in Farm Animals, pp. 346349. Ithaca, NY: Cornell University
Wheelock J. V., Rook J. A. F., Neave F. K. & Dodd F. H. 1966 The effect of bacterial infections of the udder on the yield and composition of cow's milk. Journal of Dairy Research 33 199215
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Journal of Dairy Research
  • ISSN: 0022-0299
  • EISSN: 1469-7629
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