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Comparison of proteolytic activities in various lactobacilli

Published online by Cambridge University Press:  01 June 2009

Masahiro Sasaki ,
Boukje W. Bosman  and
Paris S. T. Tan
Masahiro Sasaki
Affiliation:
Snow Brand European Research Laboratories BV, Zernikepark 6, NL-9747 AN Groningen, The Netherlands
Boukje W. Bosman
Affiliation:
Snow Brand European Research Laboratories BV, Zernikepark 6, NL-9747 AN Groningen, The Netherlands
Paris S. T. Tan
Affiliation:
Snow Brand European Research Laboratories BV, Zernikepark 6, NL-9747 AN Groningen, The Netherlands
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Summary

A total of 169 Lactobacillus strains from 12 species (Lb. acidophilus, Lb. brevis, Lb. buchneri, Lb. casei, Lb. delbrueckii subsp. bulgaricus, Lb. delbrueckii subsp. delbrueckii, Lb. delbrueckii subsp. lactis, Lb. fermentum, Lb. helveticus, Lb. paracasei subsp. paracasei, Lb. plantarum and Lb. rhamnosus), isolated from raw milk and various milk products, and 9 Lactococcus lactis strains were evaluated for peptidase activities with five chromogenic substrates and a tryptic digest of casein. Within each species, the peptidase activity of the cell-free extracts of the strains varied. Furthermore, differences were observed between the Lactobacilhis species and Lc. lactis. Lb. helveticus had by far the highest hydrolysing activities towards all substrates, indicating the presence of powerful aminopeptidases, X-prolyl-dipeptidyl aminopeptidases and proline iminopeptidases. Lb. delbrueckii subsp. bulgaricus possessed high hydrolysing activities towards substrates containing proline, alanylprolyl–p–nitroanilide and prolyl–p–nitroanilide. On the other hand, Lb. fermentum and Lb. brevis could be considered as weakly proteolytic species. A more detailed study with highly proteolytic Lactobacillus strains indicated that at least three different proteinases or endopeptidases were present. Compared with Lc. lactis, the Lactobacillus strains had a much lower hydrolytic action on glutamyl-glutamic acid, suggesting that glutamyl aminopeptidase was absent in lactobacilli.

Type
Original Articles
Information
Journal of Dairy Research , Volume 62 , Issue 4 , November 1995 , pp. 601 - 610
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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