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Comparison of the structure of micellar calcium phosphate in milk from six species by extended X-ray absorption fine structure spectroscopy

Published online by Cambridge University Press:  01 June 2009

James C. Irlam ,
Carl Holt ,
S. Samar Hasnainj  and
David W. L. Hukins
James C. Irlam
Affiliation:
Department of Medical Biophysics, University of Mancheste, Manchester M13 9PT, UK
Carl Holt
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
S. Samar Hasnainj
Affiliation:
Scientific and Engineering Research Council, Daresbury Laboratory, Warrington W A4 4AD, UK
David W. L. Hukins
Affiliation:
Department of Medical Biophysics, University of Mancheste, Manchester M13 9PT, UK
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Summary

Extended X-ray absorption fine structure spectra, above the Ca K absorption edge, were recorded from lyophilized casein micelles obtained from the milk of cow, pig, goat, rabbit and rat. A spectrum was also obtained from the calcium phosphate prepared from the casein micelles of human milk. Comparison of these spectra showed that the short-range environment of calcium in the micellar calcium phosphate was the same in all six species. Furthermore, these spectra closely resembled that of brushite (CaHP04. 2H20). However, X-ray diffraction showed no evidence for an extensive crystal lattice in micellar calcium phosphate.

Type
Original Articles
Information
Journal of Dairy Research , Volume 52 , Issue 2 , May 1985 , pp. 267 - 273
Copyright
Copyright © Proprietors of Journal of Dairy Research 1985

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References

REFERENCES

Allan, R. J. L. 1940 The estimation of phosphorus. Biochemical Journal 34 858–865CrossRefGoogle Scholar
Bianconi, A., Innoccia, L. & Stipcich, S. (Eds) 1983 EXAFS and Near Edge Structures. Berlin: Springer Verlag.CrossRefGoogle Scholar
Binsted, N., Hasnain, S. S. & Hukins, D. W. L. 1982 Developmental changes in bone mineral structure demonstrated by extended X-ray absorption fine structure (EXAFS) spectroscopy. Biochemical and Biophysical Research Communications 107 89–92CrossRefGoogle ScholarPubMed
Brown, E. H., Lehr, J. R., Smith, J. P. & Frazier, A. W. 1963 Preparation and characterisation of some calcium pyrophosphates. Journal of Agricultural and Food Chemistry 11 214–222CrossRefGoogle Scholar
Davies, D. T. & White, J. C. D. 1962 The determination of calcium and magnesium in milk and milk diffusate. Journal of Dairy Research 29 285–296CrossRefGoogle Scholar
Diakun, G. P., Greaves, G. N., Hasnain, S. S. & Quinn, P. D. 1984 X-Ray Absorption Spectroscopy, SERC Daresbury Laboratory, Warrington, Report DL SCI/TM38EGoogle Scholar
Eanes, E. D., Powers, L. & Costa, J. L. 1981 Extended X-ray absorption fine structure (EXAFS) studies on calcium in crystalline and amorphous solids of biological interest. Cell Calcium 2 251–261CrossRefGoogle Scholar
Greaves, G. N., Diakun, G. P., Quinn, P. D.,Hart, M. & Siddons, D. P. 1983 Anordersorting monochromator for synchrotron radiation. Nuclear Instruments and Methods 208 335–339CrossRefGoogle Scholar
Hasnain, S. S. 1983 EXAFS of calcium in biological systems. In In EXAFS and Near Edge Structures pp. 330–336 (Eds Bianconi, A., Innoccia, L. and Stipcich, S.). Berlin: Springer VerlagCrossRefGoogle Scholar
Holt, C. 1982 Inorganic constituents of milk III. The colloidal calcium phosphate of cow's milk. Journal of Dairy Research 49 29–38CrossRefGoogle ScholarPubMed
Holt, C., Hasnain, S. S. & Hukins, D. W. L. 1982 Structure of bovine milk calcium phosphate determined by X-ray absorption spectroscopy. Biochimica el Biophysica Acta 719 299–303CrossRefGoogle ScholarPubMed
Holt, C. & Jenness, R. 1984 Interrelationships of constituents and partition of salts in milk samples from eight species. Comparative Biochemistry and Physiology A 77 275–282CrossRefGoogle ScholarPubMed
Hukins, D. W. L. 1981 X-Ray Diffraction by Disordered and Ordered Systems. Oxford: Pergamon PressGoogle Scholar
Hukins, D. W. L., Hickey, D. S., & Kennedy, A. P. 1983 Catheter encrustation by struvite. British Journal of Urology 55 304–305CrossRefGoogle ScholarPubMed
Jenness, R. 1979 Comparative aspects of milk proteins. Journal of Dairy Research 46 197–210CrossRefGoogle ScholarPubMed
Knoop, A. M., Knoop, E. & Wiechen, A. 1979 a Sub-structure of synthetic casein micelles. Journal of Dairy Research 46 347–350CrossRefGoogle ScholarPubMed
Knoop, A. M., Knoop, E., Frede, E., Precht, D. & Wiechen, A. 1979 b [The submicroscopic structure of natural and artificial casein micelles] Milchwissenschaft 34 129–131Google Scholar
McGann, T. C. A., Kearney, R. D., Buchheim, W., Posner, A. S., Betts, F. & Blumenthal, N. C. 1983 Amorphous calcium phosphate in casein micelles of bovine milk. Calcified Tissue International 35 821–823CrossRefGoogle ScholarPubMed
Miller, R. M., Hukins, D. W. L., Hasnain, S. S. & Lagarde, P. 1981 Extended X-ray absorption fine structure (EXAFS) studies of the calcium ion environment in bone mineral and related calcium phosphates. Biochemical and Biophysical Research Communications 99 102–106CrossRefGoogle ScholarPubMed
Rose, D. & Colvin, J. R. 1966 Internal structure of casein micelles from bovine milk. Journal of Dairy Science 49 351–355CrossRefGoogle ScholarPubMed
Schmidt, D. G. 1982 Association of caseins and casein micelle structure. In Developments in Dairy Chemistry - 1 Proteins pp. 61–86 (Ed. Fox, P. F.) London: Applied ScienceGoogle Scholar
Teo, B. K. 1981 Extended X-ray absorption fine structure (EXAFS) spectroscopy: techniques and applications. In EXAFS Spectroscopy Techniques and Applications (Eds Teo, B. K. and Joy, D. C.) New York: Plenum PressCrossRefGoogle Scholar