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Detection of two SNPs of the LIPE gene in Holstein–Friesian cows with divergent milk production

Published online by Cambridge University Press:  24 August 2023

Seham El-Kassas ,
Haitham G. Abo-Al-Ela Open the ORCID record for Haitham G. Abo-Al-Ela [Opens in a new window] ,
Esraa Abdulraouf ,
Mohamed Atef Helal ,
A. M. Sakr  and
Safaa E. Abdo
Seham El-Kassas
Affiliation:
Animal, Poultry and Fish Breeding and Production, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt
Haitham G. Abo-Al-Ela*
Affiliation:
Genetics and Biotechnology, Department of Aquaculture, Faculty of Fish Resources, Suez University, Suez, 43518, Egypt
Esraa Abdulraouf
Affiliation:
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt
Mohamed Atef Helal
Affiliation:
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt
A. M. Sakr
Affiliation:
Animal Production Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
Safaa E. Abdo
Affiliation:
Genetics and Genetic Engineering, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt
*
Corresponding author: Haitham G. Abo-Al-Ela; Email: haitham.aboalela@frc.suezuni.edu.eg
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Abstract

The LIPE gene (lipase E, hormone-sensitive type), also known as hormone-sensitive lipase, acts as a primary regulator of lipid metabolism during lactation in cows. We studied a total of two hundred Holstein–Friesian cows and performed sequencing analysis that revealed two synonymous nucleotide changes within the LIPE gene: a transition change, c.276 T > C in exon 2 (g.50631651 T > C; position 351 of GenBank: ON638900) and a transversion change, c.219C > A in exon 6 (g.50635369C > A; position 1070 of GenBank: ON638901). The observed genotypes were TC and CC for the c.276 T > C SNP and CC and CA for the c.219C > A SNP. Notably, the heterozygous TC genotype of the T351C SNP exhibited a significant association with high milk yield. Furthermore, the T351C SNP displayed significant associations with various milk parameters, including temperature, freezing point, density and the percentages of fat, protein, lactose, solids and solids-not-fat, with the homozygous CC genotype showing higher values. The c.219C > A SNP also demonstrated a significant association with milk composition, with heterozygous genotypes (CA) exhibiting higher percentages of fat, protein, and lactose compared to homozygous genotypes (CC). This effect was consistent among both high and low milk producers for fat and lactose percentages, while high milk producers exhibited a higher protein percentage than low milk producers. These findings highlight the importance of considering the detected SNPs in marker-assisted selection and breeding programs for the identification of high milk-producing Holstein–Friesian cows and potentially other breeds. Moreover, this study strongly supports the fundamental role of the LIPE gene in milk production and composition in lactating animals.

Keywords

CattleLIPEmarker-assisted selectionmilk productionpolymorphismSNPs
Type
Research Article
Information
Journal of Dairy Research , Volume 90 , Issue 3 , August 2023 , pp. 244 - 251
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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References

Abou-Bakr, S (2009) Genetic and phenotypic trends of 305-day milk yield of Holstein cows raised at commercial farm in Egypt. Egyptian Journal of Animal Production 46, 85–92.Google Scholar
Aboulaich, N, Örtegren, U, Vener, AV and Strålfors, P (2006) Association and insulin regulated translocation of hormone-sensitive lipase with PTRF. Biochemical and Biophysical Research Communications 350, 657–661.CrossRefGoogle ScholarPubMed
Aboulaich, N, Chui, PC, Asara, JM, Flier, JS and Maratos-Flier, E (2011) Polymerase I and transcript release factor regulates lipolysis via a phosphorylation-dependent mechanism. Diabetes 60, 757–765.CrossRefGoogle Scholar
Brule, CE and Grayhack, EJ (2017) Synonymous codons: choose wisely for expression. Trends in Genetics 33, 283–297.CrossRefGoogle ScholarPubMed
Contreras, GA, Strieder-Barboza, C and Raphael, W (2017) Adipose tissue lipolysis and remodeling during the transition period of dairy cows. Journal of Animal Science and Biotechnology 8, 41.CrossRefGoogle ScholarPubMed
De Koster, J, Nelli, RK, Strieder-Barboza, C, de Souza, J, Lock, AL and Contreras, GA (2018) The contribution of hormone sensitive lipase to adipose tissue lipolysis and its regulation by insulin in periparturient dairy cows. Scientific Reports 8, 13378.CrossRefGoogle ScholarPubMed
Fang, X, Zhang, L, Yu, X, Li, JY, Lu, CY, Zhao, ZH and Yang, R (2014) Association of HSL gene E1-c. 276C> T and E8-c. 51C> T mutation with economical traits of Chinese Simmental cattle. Molecular Biology Reports 41, 105–112.CrossRefGoogle ScholarPubMed
Fang, X, Zhao, Z, Jiang, P, Yu, H, Xiao, H and Yang, R (2017) Identification of the bovine HSL gene expression profiles and its association with fatty acid composition and fat deposition traits. Meat Science 131, 107–118.CrossRefGoogle ScholarPubMed
FAO (2019) The State of Food and Agriculture 2019. Moving forward on food loss and waste reduction. Rome. Licence: CC BY-NC-SA 3.0 IGO.Google Scholar
Greenfield, RB, Cecava, MJ and Donkin, SS (2000a) Changes in mRNA expression for gluconeogenic enzymes in liver of dairy cattle during the transition to lactation. Journal of Dairy Science 83, 1228–1236.CrossRefGoogle ScholarPubMed
Greenfield, RB, Cecava, MJ, Johnson, TR and Donkin, SS (2000b) Impact of dietary protein amount and rumen undegradability on intake, peripartum liver triglyceride, plasma metabolites, and milk production in transition dairy cattle. Journal of Dairy Science 83, 703–710.CrossRefGoogle ScholarPubMed
Gui, L-S, Raza, SHA, Memon, S, Li, Z, Abd El-Aziz, AH, Ullah, I, Jahejo, AR, Shoorei, H, Khan, R, Quan, G and Liu, GY (2020) Association of hormone-sensitive lipase (HSL) gene polymorphisms with the intramuscular fat content in two Chinese beef cattle breeds. Genomics 112, 3883–3889.CrossRefGoogle ScholarPubMed
Gustafsson, C, Govindarajan, S and Minshull, J (2004) Codon bias and heterologous protein expression. Trends in Biotechnology 22, 346–353.CrossRefGoogle ScholarPubMed
Karki, R, Pandya, D, Elston, RC and Ferlini, C (2015) Defining “mutation” and “polymorphism” in the era of personal genomics. BMC Medical Genomics 8, 37.CrossRefGoogle ScholarPubMed
Khan, MJ, Hosseini, A, Burrell, S, Rocco, SM, McNamara, JP and Loor, JJ (2013) Change in subcutaneous adipose tissue metabolism and gene network expression during the transition period in dairy cows, including differences due to sire genetic merit. Journal of Dairy Science 96, 2171–2182.CrossRefGoogle ScholarPubMed
Komar, AA (2007) SNPs, silent but not invisible. Science (New York, N.Y.) 315, 466–467.CrossRefGoogle Scholar
Kong, Y, Yuan, Z, Liu, X, Li, F and Yue, X (2022) A novel SNP within LIPE gene is highly associated with sheep intramuscular fat content. Small Ruminant Research 209, 106658.CrossRefGoogle Scholar
Kraemer, FB and Shen, W-J (2002) Hormone-sensitive lipase. Journal of Lipid Research 43, 1585–1594.CrossRefGoogle ScholarPubMed
Kraemer, FB and Shen, W-J (2006) Hormone-sensitive lipase knockouts. Nutrition & Metabolism 3, 12.CrossRefGoogle ScholarPubMed
Lampidonis, AD, Argyrokastritis, A, Stravopodis, DJ, Voutsinas, GE, Ntouroupi, TG, Margaritis, LH, Bizelis, I and Rogdakis, E (2008) Cloning and functional characterization of the ovine Hormone Sensitive Lipase (HSL) full-length cDNAs: an integrated approach. Gene 416, 30–43.CrossRefGoogle ScholarPubMed
Martín-Hidalgo, A, Huerta, L, Álvarez, N, Alegría, G, del Val Toledo, M and Herrera, E (2005) Expression, activity, and localization of hormone-sensitive lipase in rat mammary gland during pregnancy and lactation. Journal of Lipid Research 46, 658–668.CrossRefGoogle ScholarPubMed
McNamara, JP, McFarland, DC and Bai, S (1987) Regulation of bovine adipose tissue metabolism during lactation. 3. Adaptations of hormone-sensitive and lipoprotein lipases. Journal of Dairy Science 70, 1377–1384.CrossRefGoogle ScholarPubMed
Pizarro, MG, Landi, V, Navas, FJ, León, JM, Martínez, A, Fernández, J and Delgado, JV (2020) Non-parametric analysis of the effects of nongenetic factors on milk yield, fat, protein, lactose, dry matter content and somatic cell count in Murciano-Granadina goats. Italian Journal of Animal Science 19, 960–973.CrossRefGoogle Scholar
Plotkin, JB and Kudla, G (2011) Synonymous but not the same: the causes and consequences of codon bias. Nature Reviews Genetics 12, 32–42.CrossRefGoogle Scholar
Robert, F and Pelletier, J (2018) Exploring the impact of single-nucleotide polymorphisms on translation. Frontiers in Genetics 9, 507.CrossRefGoogle ScholarPubMed
Saltiel, AR (2000) Another hormone-sensitive triglyceride lipase in fat cells? Proceedings of the National Academy of Sciences 97, 535–537.CrossRefGoogle ScholarPubMed
Shatoff, E and Bundschuh, R (2020) Single nucleotide polymorphisms affect RNA-protein interactions at a distance through modulation of RNA secondary structures. PLOS Computational Biology 16, e1007852.CrossRefGoogle Scholar
Singh, V and Gupta, J (2016) Factors affecting raw milk quality in dairy value chain in Rajasthan (India): a comprehensive study. Indian Journal of Animal Sciences 86, 1204–1209.Google Scholar
Waldman, YY, Tuller, T, Keinan, A and Ruppin, E (2011) Selection for translation efficiency on synonymous polymorphisms in recent human evolution. Genome Biology and Evolution 3, 749–761.CrossRefGoogle ScholarPubMed
Yonezawa, T, Haga, S, Kobayashi, Y, Katoh, K and Obara, Y (2008) Regulation of hormone-sensitive lipase expression by saturated fatty acids and hormones in bovine mammary epithelial cells. Biochemical and Biophysical Research Communications 376, 36–39.CrossRefGoogle ScholarPubMed
Zachut, M (2015) Defining the adipose tissue proteome of dairy cows to reveal biomarkers related to peripartum insulin resistance and metabolic status. Journal of Proteome Research 14, 2863–2871.CrossRefGoogle ScholarPubMed
Zidi, A, Fernández-Cabanás, VM, Carrizosa, J, Jordana, J, Urrutia, B, Polvillo, O, González-Redondo, P, Gallardo, D, Amills, M and Serradilla, JM (2010) Genetic variation at the goat hormone-sensitive lipase (LIPE) gene and its association with milk yield and composition. Journal of Dairy Research 77, 190–198.CrossRefGoogle ScholarPubMed
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