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Development of a cows' milk identification test (COMIT) for field use

Published online by Cambridge University Press:  01 June 2009

Teresa García ,
Rosario Martín ,
Elena Rodríguez ,
Pablo E. Hernández  and
Bernabé Sanz
Teresa García
Affiliation:
Departamento de Higiene y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
Rosario Martín
Affiliation:
Departamento de Higiene y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
Elena Rodríguez
Affiliation:
Departamento de Higiene y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
Pablo E. Hernández
Affiliation:
Departamento de Higiene y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
Bernabé Sanz
Affiliation:
Departamento de Higiene y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
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Summary

A cows' milk identification test (COMIT) has been successfully developed for the detection of defined amounts of cows' milk (3–100%) in ewes' milk. The assay uses polyclonal antibodies raised in goats against bovine whey proteins. The test, an agar-gel immunodiffusion technique, uses agar plates with a printed template for correct placement of the test components (antisera discs, positive and negative milk reference discs and sample discs). The presence of cows' milk in the sample was recorded as positive when the precipitin lines appearing between the sample disc and the antisera disc fuse completely with those from the positive cows' milk reference disc. This test is reliable, practical and easy to perform in the field and in dairy factories. Furthermore, the nature and stability of the components of the test make it suitable for commercial development into kits which should be highly practical in any kind of inspection programme concerned with milk species identification.

Type
Original articles
Information
Journal of Dairy Research , Volume 56 , Issue 5 , November 1989 , pp. 691 - 698
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

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