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Development of bacterial biofilms in dairy processing lines

Published online by Cambridge University Press:  01 June 2009

John W. Austin  and
Gilles Bergeron
John W. Austin
Affiliation:
Microbiology Research Division, Bureau of Microbial Hazards, Food Directorate, Health Protection Branch, Health Canada, Sir Frederick Banting Research Centre, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2
Gilles Bergeron
Affiliation:
Microbiology Research Division, Bureau of Microbial Hazards, Food Directorate, Health Protection Branch, Health Canada, Sir Frederick Banting Research Centre, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2
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Summary

Adherence of bacteria to various milk contact sites was examined by scanning electron microscopy and transmission electron microscopy. New gaskets, endcaps, vacuum breaker plugs and pipeline inserts were installed in different areas in lines carrying either raw or pasteurized milk, and a routine schedule of cleaning-in-place and sanitizing was followed. Removed cleaned and sanitized gaskets were processed for scanning or transmission electron microscopy. Adherent bacteria were observed on the sides of gaskets removed from both pasteurized and raw milk lines. Some areas of Buna-n gaskets were colonized with a confluent layer of bacterial cells surrounded by an extensive amorphous matrix, while other areas of Buna-n gaskets showed a diffuse adherence over large areas of the surface. Most of the bacteria attached to polytetrafluoroethylene (PTFE or Teflon™) gaskets were found in crevices created by insertion of the gasket into the pipeline. Examination of stainless steel endcaps, pipeline inserts, and PTFE vacuum breaker plugs did not reveal the presence of adherent bacteria. The results of this study indicate that biofilms developed on the sides of gaskets in spite of cleaning-in-place procedures. These biofilms may be a source of post-pasteurization contamination.

Type
Original Articles
Information
Journal of Dairy Research , Volume 62 , Issue 3 , August 1995 , pp. 509 - 519
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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References

REFERENCES

Austin, J. W., Stewart, M. & Muhray, R. G. E. 1990 Structural and chemical characterization of the S layer of a Pseudomonas-Wke bacterium. Journal of Bacteriology 172 808–817CrossRefGoogle Scholar
Carpentier, B. & Cere, O. 1993 Biofilms and their consequences, with particular reference to hygiene in the food industry. Journal of Applied Bacteriology 75 499–511CrossRefGoogle ScholarPubMed
Characklis, W. G. 1981 Fouling biofilm development: a process analysis. Biotechnology and Bioengineering 23 1923–1960CrossRefGoogle Scholar
Czechowski, M. H. 1990 Bacterial attachment to Buna-n gaskets in milk processing equipment. Australian Journal of Dairy Technology 45 113–114Google Scholar
Dekker, N. P., Lammel, C. J. & Brooks, G. F. 1991 Scanning electron microscopy of piliated Neisseria gonorrhoeae processed with hexamethyldisilazane. Journal of Electron Microscopy Technique 19 461–467CrossRefGoogle ScholarPubMed
Dunsmore, D. G. 1981 Bacteriological control of food equipment surfaces by cleaning systems. I. Detergent effects. Journal of Food Protection 44 15–20CrossRefGoogle ScholarPubMed
Dunsmore, D. G., Twomey, A., Whittlestone, W. G. & Morgan, H. W. 1981 Design and performance of systems for cleaning product-contact surfaces of food equipment. A review. Journal of Food Protection 44 220–240CrossRefGoogle ScholarPubMed
Frank, J. F. & Koffi, R. A. 1990 Surface adherent growth of Listeria monocytogenes is associated with increased resistance to surface sanitisers and heat. Journal of Food Protection 53 550–554CrossRefGoogle Scholar
Garvie, E. I. & Clarke, P. M. 1955 The disinfectant properties of quaternary ammonium compounds and sodium hypochlorite: a comparison of results using different test methods. Journal of Applied Bacteriology 18 90–106CrossRefGoogle Scholar
Helke, D. M., Somers, E. B. & Wong, A. C. L. 1993 Attachment of Listeria monocytogenes and Salmonella typhimurium to stainless steel and Buna-N in the presence of milk and individual milk components. Journal of Food Protection 56 479–484CrossRefGoogle ScholarPubMed
Holah, J. T. & Thorpe, R. H. 1990 Cleanability in relation to bacterial retention on unused and abraded domestic sink materials. Journal of Applied Bacteriology 69 599–608CrossRefGoogle ScholarPubMed
Koutzayiotis, C. 1992 Bacterial biofilms in milk pipelines. South African Journal of Dairy Science 24 19–22Google Scholar
Krysinski, E. P., Brown, L. J. & Marchisello, T. J. 1992 Effect of cleaners and sanitizers on Listeria monocytogenes attached to product contact surfaces. Journal of Food Protection 55 246–251CrossRefGoogle ScholarPubMed
LeChevallier, M. W., Cawthon, C. D. & Lee, R. G. 1988 a Factors promoting survival of bacteria in chlorinated water supplies. Applied and Environmental Microbiology 54 649–654CrossRefGoogle ScholarPubMed
Lechevallier, M. W., Cawthon, C. D. & Lee, R. G. 1988 b Inactivation of biofilm bacteria. Applied and Environmental Microbiology 54 2492–2499CrossRefGoogle ScholarPubMed
Mattila, T., Manninen, M. & Kyläsiurola, A. -L. 1990 Effect of cleaning-in-place disinfectants on wild bacterial strains isolated from a milking line. Journal of Dairy Research 57 33–39CrossRefGoogle ScholarPubMed
Mattila-Sandholm, T. & Wirtanen, G. 1992 Biofilm formation in the industry. A review. Food Reviews International 8 573–603CrossRefGoogle Scholar
Maxcy, R. B. 1964 Potential microbial contaminants from dairy equipment with automated circulation cleaning. Journal of Milk and Food Technology 27 135–139CrossRefGoogle Scholar
Maxcy, R. B. 1969 Residual micro-organisms in cleaned-in-place systems for handling milk. Journal of Milk and Food Technology 32 140–143CrossRefGoogle Scholar
Muir, D. D. 1990 The microbiology of heat-treated fluid milk products. In Dairy Microbiology, vol. 1 The Microbiology of Milk, 2nd edn, pp. 209–243 (Ed. Robinson, R. K.). London: Elsevier Applied ScienceGoogle Scholar
Nickel, J. C., Downey, J. & Costerton, J. W. 1992 Movement of Pseudomonas aeruginosa along catheter surfaces. A mechanism in pathogenesis of catheter-associated infection. Urology 39 93–98CrossRefGoogle ScholarPubMed
Nickel, J. C., Gristina, A. G. & Costerton, J. W. 1985 Electron microscopic study of an infected Foley catheter. Canadian Journal of Surgery 28 50–52Google ScholarPubMed
Phillips, J. D. & Griffiths, M. W. 1990 Pasteurized dairy products. The constraints imposed by environmental contamination. In Food. Contamination from Environmental Sources, pp. 387–456 (Eds Nriagu, J. O. and Simmons, M. S.). New York: John Wiley and Sons (Advances in Environmental Science and Technology vol. 23)Google Scholar
Phillips, J. D., Griffiths, M. W. & Muir, D. D. 1981 Factors affecting the shelf-life of pasteurized double cream. Journal of the Society of Dairy Technology 34 109–113CrossRefGoogle Scholar
Reynolds, E. S. 1963 The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17 208–212CrossRefGoogle ScholarPubMed
Sasahara, K. C. & Zottola, E. A. 1993 Biofilm formation by Listeria, monocytogenes utilizes a primary colonizing microorganism in flowing systems. Journal of Food Protection 56 1022–1028CrossRefGoogle ScholarPubMed
Schröder, M. J. A. 1984 Origins and levels of post pasteurization contamination of milk in the dairy and their effects on keeping quality. Journal of Dairy Research 51 59–67CrossRefGoogle ScholarPubMed
Schröder, M. J. A., Cousins, C. M. & McKinnon, C. H. 1982 Effect of psychrotrophic post-pasteurization contamination on the keeping quality at 11 and 5°C of HTST-pasteurized milk in the UK. Journal of Dairy Research 49 619–630CrossRefGoogle Scholar
Shin-Ho-Lee, & Frank, J. F. 1991 Inaotivation of surface-adherent Listeria monocytogenes by hypochlorite and heat. Journal of Food Protection 54 4–6Google Scholar