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Effect of green silver nanoparticles on the viability and adherence of common bovine mastitis pathogens

Published online by Cambridge University Press:  15 October 2025

Anike Virginia Ferreyra Maillard ,
Alejandro Fili ,
Pablo Dalmasso ,
Claudia Raspanti  and
Matias Santiago Pellegrino
Anike Virginia Ferreyra Maillard*
Affiliation:
NOA Institute of Bionanotechnology (INBIONATEC), National University of Santiago del Estero (UNSE), CONICET, Santiago del Estero, Argentina Center for Applied Biophysics and Food Research (CIBAAL), National University of Santiago del Estero (UNSE), CONICET, Santiago del Estero, Argentina
Alejandro Fili
Affiliation:
Department of Molecular Biology, Faculty of Exact, Physico-Chemical and Natural Sciences, National University of Río Cuarto, Río Cuarto, Córdoba, Argentina
Pablo Dalmasso
Affiliation:
Department of Chemical Engineering, Regional Faculty of Córdoba, Center for Research and Transfer in Environmental Chemical Engineering (CIQA), National Technological University, CONICET, Córdoba, Argentina Member of National Scientific and Technological Research Council (CONICET), CABA, Buenos Aires, Argentina
Claudia Raspanti
Affiliation:
Department of Microbiology and Immunology, Faculty of Exact, Physical, Physical-Chemical and Natural Sciences, National University of Río Cuarto, Río Cuarto, Córdoba, Argentina
Matias Santiago Pellegrino
Affiliation:
Member of National Scientific and Technological Research Council (CONICET), CABA, Buenos Aires, Argentina Department of Microbiology and Immunology, Faculty of Exact, Physical, Physical-Chemical and Natural Sciences, National University of Río Cuarto, Río Cuarto, Córdoba, Argentina
*
Corresponding author: Matias Santiago Pellegrino; Email: mpellegrino@exa.unrc.edu.ar
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Abstract

This communication addresses the hypothesis that silver nanoparticles obtained by green synthesis (G-AgNPs), using an aqueous extract of chicory, have antibacterial activity against bovine mastitis-associated strains and may inhibit their adherence capacity. Bovine mastitis is among the diseases with the greatest economic impact worldwide. Multifactorial in nature, it is usually associated with infection caused by bacterial species such as Staphylococcus aureus, coagulase-negative staphylococci, Streptococcus uberis, and Escherichia coli. Antibiotics are currently the main line of treatment, but in order to offset the emergence of resistance, alternative compounds and nanomaterials are being researched for prevention and control. In this study, bacteria that cause bovine mastitis were molecularly identified by groEL gene PCR-RFLP. Then, G-AgNPs were produced using an aqueous extract of chicory as a reducing and stabilizing agent. The nanoparticles showed powerful antibacterial activity, but they did not seem to reduce the adherence of pathogens to MAC-T cells that had been preincubated with a sub-MIC concentration of G-AgNPs. Nevertheless, very low concentrations (21-30 pM) were needed for the antibacterial effect to take place, which makes G-AgNPs promising candidates for the control of bovine mastitis.

Keywords

antimicrobial activitysbacterial adherencebovine mastitispreventionsilver nanoparticles

Information

Type
Research Article
Information
Journal of Dairy Research , First View , pp. 1 - 4
Copyright
© The Author(s), 2025. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

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