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The effect of probiotic fermented milk and inulin on the functions and microecology of the intestine

  • Ulla Sairanen (a1), Laura Piirainen (a2), Soile Gråsten (a3), Tuomo Tompuri (a3) (a4), Jaana Mättö (a5), Maria Saarela (a5) and Riitta Korpela (a1) (a6) (a7)...

Abstract

We investigated the effects of a probiotic fermented milk and inulin on gastrointestinal function and microecology. The study was double-blinded and comprised 66 healthy adults (22 male, 44 female), mean age 40 years (range, 22–60 years). After a 12-d baseline period the subjects were randomized to consume, for 3 weeks, 3×200 ml daily of either (1) a fermented milk with probiotics (Bifidobacterium longum BB536, Bifidobacterium spp. 420 and Lactobacillus acidophilus 145), (2) a fermented milk with the same probiotics plus 4 g inulin, or (3) a control fermented milk. During the last 7 d of the baseline and the intervention periods, the subjects kept a record of their defaecation frequency and gastrointestinal symptoms, and collected all their faeces. Intestinal transit time, stool weight and faecal enzyme activities were measured. Thirty-nine subjects were randomized to give faecal samples for analysis of pH and microbes, including lactobacilli, bifidobacteria, coliforms, Escherichia coli, Bacteroides and Clostridium perfringens. Consumption of fermented milk with probiotics or with probiotics and inulin increased the faecal number of lactobacilli (P=0·009, P=0·003) and bifidobacteria (P=0·046, P=0·038) compared with the baseline. Compared with the control fermented milk, both active products increased lactobacilli (P=0·005, ANCOVA). Subjects consuming fermented milk with probiotics and inulin suffered from gastrointestinal symptoms, especially flatulence, more than the others (P<0·001). In conclusion, the probiotic fermented milk product had a positive effect by increasing the number of lactobacilli and bifidobacteria in the colon. Inulin did not alter this effect but it increased gastrointestinal symptoms.

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Corresponding author

*For correspondence; e-mail: riitta.korpela@valio.fi

References

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Alander, M, Mättö, J, Kneifel, W, Johansson, M, Kögler, B, Crittenden, R, Mattila-Sandholm, T & Saarela, M 2001 Effect of galacto-oligosaccharide supplementation on human faecal microflora and on survival and persistence of Bifidobacterium lactis Bb-12 in the gastrointestinal tract. International Dairy Journal 11 817825
Alles, MS, Hautvast, JGAJ, Nagengast, FM, Hartemink, R, van Laere, KMJ & Jansen, JBMJ 1996 Fate of fructo-oligosaccharides in the human intestine. British Journal of Nutrition 76 211221
Beerens, H 1991 Detection of bifidobacteria by using propionic acid as a selective agent. Applied and Environmental Microbiology 57 24182419
Brighenti, F, Casiraghi, MC, Canzi, E & Ferrari, A 1999 Effect of consumption of a ready-to-eat breakfast cereal containing inulin on the intestinal milieu and blood lipids in healthy male volunteers. European Journal of Clinical Nutrition 53 726733
Bouhnik, Y, Flourie, B, Anderieux, C, Bisetti, N, Briet, F & Ranbaud, JC 1996 Effects of Bifidobacterium sp. fermented milk ingested with or without inulin on colonic bifidobacteria and enzymatic activities in healthy humans. European Journal of Clinical Nutrition 50 269273
Freeman, HJ 1986 Effects of differing purified cellulose pectin, and hemicellulose fibre on fecal enzymes in 1·2-dimethyl-hydrazine-induced rat colon carsinogenesis. Cancer Research 46 55295532
Gibson, GR, Beatty, ER, Wang, X & Cummings, JH 1995 Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 108 975982
Gibson, GR & Roberfroid, MB 1995 Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. Journal of Nutrition 125 14011412
Gibson, GR & Wang, X 1994 Regulatory effects of bifidobacteria on the growth of other colonic bacteria. Journal of Applied Bacteriology 77 412420
Goossens, D, Jonkers, D, Russel, M, Stobberingh, E, Van Den Bogaard, A & Stockbrugger, R 2003 The effect of Lactobacillus plantarum 299v on the bacterial composition and metabolic activity in faeces of healthy volunteers: a placebo-controlled study on the onset and duration of effects. Alimentary Pharmacology and Therapeutics 18 495505
Gorbach, SL & Goldin, BR 1990 The intestinal microflora and the colon cancer connection. Reviews of Infection and Disease 12 Suppl 2, S252S261
Hinton, JM, Lennard-Jones, JE & Young, AC 1969 A new method for studying gut transit times using radioopaque markers. Gut 10 842847
Jäkälä, E, Korpela, R & Mykkänen, H 1994 Validity and reliability of a short interview method in measuring the intake of dietary fibre. Scandinavian Journal of Nutrition 38 (Suppl 29) 5
Kleessen, B, Sykura, B, Zunft, HJ & Blaut, M 1997 Effects of inulin and lactose on fecal microflora, microbial activity, and bowel habit in elderly constipated persons. American Journal of Clinical Nutrition 65 13971402
Klinder, A, Forster, A, Caderni, G, Femia, AP & Pool-Zobel, BL 2004 Fecal water genotoxicity is predictive of tumor-preventive activities by inulin-like oligofructoses, probiotics (Lactobacillus rhamnosus and Bifidobacterium lactis), and their synbiotic combination. Nutrition and Cancer 49 144155
Kruse, HP, Kleessen, B & Blaut, M 1999 Effects of inulin on faecal bifidobacteria in human subjects. British Journal of Nutrition 82 375382
Langlands, SJ, Hopkins, MJ, Coleman, N & Cummings, JH 2004 Prebiotic carbohydrates modify the mucosa associated microflora of the human large bowel. Gut 53 16101616
Ling, WH, Korpela, R, Mykkänen, H, Salminen, S & Hänninen, O 1994 Lactobacillus strain GG supplementation decreases colonic hydrolytic and reductive enzyme activities in healthy female adults. Journal of Nutrition 124 1823
McBain, AJ & Macfarlane, GT 2001 Modulation of genotoxic enzyme activities by non-digestible oligosaccharide metabolism in in-vitro human gut bacterial ecosystems. Journal of Medical Microbiology 50 833842
Nurmi, JT, Puolakkainen, PA & Rautonen, NE 2005 Bifidobacterium Lactis sp. 420 up-regulates cyclooxygenase (Cox)-1 and down-regulates Cox-2 gene expression in a Caco-2 cell culture model. Nutrition and Cancer 51 8392
Ogata, T, Kingaku, M, Yaeshima, T, Teraguchi, S, Fukuwataru, Y, Ishibashi, N, Hayasawa, H, Fujisawa, T & Iino, H 1999 Effect of Bifidobacterium longum BB536 yogurt administration on the intestinal environment of healthy adults. Microbial Ecology in Health and Disease 11 4146
Ogata, T, Nakamura, T, Anjitsu, K, Yaeshima, T, Takahashi, S & Fukuwatari, Y 1997 Effect of Bifidobacterium longum BB536 administration on the intestinal environment, defecation frequency and fecal characteristics of human volunteers. Bioscience and Microflora 16 5358
Pedersen, A, Sandström, B & van Amelsvoort, JMM 1997 The effect of ingestion of inulin on blood lipids and gastrointestinal symptoms in healthy females. British Journal of Nutrition 78 215222
Reddy, BS & Riverson, A 1993 Inhibitory effect of Bifidobacterium longum on colon, mammary and liver carcinogenesis induced by 2-amino-3-methylimidazo[4,5-f]quinoline, a food mutagen. Cancer Research 53 39143918
Roberfroid, M 1993 Dietary fiber, inulin, and oligofructose: a review comparing their physiological effects. Critical Reviews in Food Science and Nutrition 33 103148
Roberfroid, MB 1998 Prebiotics and synbiotics: concepts and nutritional properties. British Journal of Nutrition 80 S197S202
Roller, M, Pietro Femia, A, Caderni, G, Rechkemmer, G & Watzl, B 2004 Intestinal immunity of rats with colon cancer is modulated by oligofructose-enriched inulin combined with Lactobacillus rhamnosus and Bifidobacterium lactis. British Journal of Nutrition 92 931938
Rowland, IR, Rumney, CJ, Coutts, JT & Lievense, LC 1998 Effect of Bifidobacterium longum and inulin on gut bacterial metabolism and carcinogen-induced aberrant crypt foci in rats. Carcinogenesis 19 281285
Singh, J, Rivenson, A, Tomita, M, Shimamura, S, Ishibashi, N & Reddy, BS 1997 Bifidobacterium longum, a lactic acid producing intestinal bacterium inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis. Carcinogenesis 18 833841
Sobotka, L, Bratova, M, Slemrova, M, Manak, J, Vizda, J & Zadak, Z 1997 Inulin as the soluble fiber in liquid enteral nutrition. Nutrition 13 2125
Tsujii, M, Kawano, S, Tsuji, S, Takei, Y, Tamura, K, Fusamoto, H & Kamada, T 1995 Mechanism for ammonia-induced promotion of gastric carcinogenesis in rats. Carcinogenesis 16 563566
Wang, X & Gibson, GR 1993 Effects of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestine. Journal of Applied Bacteriology 75 373380
Welters, CF, Heineman, E, Thunnissen, FB, van den Bogaard, AE, Soeters, PB & Baeten, CG 2002 Effect of dietary inulin supplementation on inflammation of pouch mucosa in patients with an ileal pouch-anal anastomosis. Diseases of the Colon and Rectum 45 621627
Yaeshima, T, Takahashi, S, Matsumoto, N, Ishibashi, N, Hayasawa, H & Iino, Hisakazu 1997 Effect of yoghurt containing Bifidobacterium longum BB536 on the intestinal environment, fecal characteristics and defecation frequency: a comparison with standard yoghurt. Bioscience and Microflora 16 7377
Zsivkovits, M, Fekadu, K, Sontag, G, Nabinger, U, Huber, WW, Kundi, M, Chakraborty, A, Foissy, H & Knasmuller, S 2003 Prevention of heterocyclic amine-induced DNA damage in colon and liver of rats by different lactobacillus strains. Carcinogenesis 24 19131918

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The effect of probiotic fermented milk and inulin on the functions and microecology of the intestine

  • Ulla Sairanen (a1), Laura Piirainen (a2), Soile Gråsten (a3), Tuomo Tompuri (a3) (a4), Jaana Mättö (a5), Maria Saarela (a5) and Riitta Korpela (a1) (a6) (a7)...

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