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    Brinkmann, J. Jagannathan, V. Drögemüller, C. Rieder, S. Leeb, T. Thaller, G. and Tetens, J. 2016. Genetic variability of the equine casein genes. Journal of Dairy Science, Vol. 99, Issue. 7, p. 5486.

    Enjapoori, Ashwantha Kumar Lefèvre, Christophe M. Nicholas, Kevin R. and Sharp, Julie A. 2015. Hormonal regulation of platypus Beta-lactoglobulin and monotreme lactation protein genes. General and Comparative Endocrinology,

    Kishore, Amit Mukesh, M. Sobti, R.C. Kataria, R.S. Mishra, B.P. and Sodhi, Monika 2014. Analysis of genetic variations across regulatory and coding regions of kappa-casein gene of Indian native cattle (Bos indicus) and buffalo (Bubalus bubalis). Meta Gene, Vol. 2, p. 769.

    Pauciullo, A. Giambra, I.J. Iannuzzi, L. and Erhardt, G. 2014. The β-casein in camels: molecular characterization of the CSN2 gene, promoter analysis and genetic variability. Gene, Vol. 547, Issue. 1, p. 159.

    Hobor, S. Kunej, T. and Dovc, P. 2008. Polymorphisms in thekappa casein(CSN3) gene in horse and comparative analysis of its promoter and coding region. Animal Genetics, Vol. 39, Issue. 5, p. 520.

    Riley, Lisa G Williamson, Peter Wynn, Peter C and Sheehy, Paul A 2008. Lactoferrin decreases primary bovine mammary epithelial cell viability and casein expression. Journal of Dairy Research, Vol. 75, Issue. 02,


Functional study of the equine β-casein and κ-casein gene promoters

  • Tina Lenasi (a1), Nadja Kokalj-Vokac (a2), Mojca Narat (a1), Antonella Baldi (a3) and Peter Dovc (a1)
  • DOI:
  • Published online: 14 July 2005

Casein genes are expressed in a tissue-specific and highly coordinated manner. The main goals of casein gene promoter studies are to unravel cis- and trans-acting factors involved in the complex signalling pathway controlling milk production, and to explore the possibility of using these promoters for tissue-specific production of heterologous proteins in the mammary gland. Here we present a comparative study of the equine β-casein and κ-casein gene proximal promoters. In order to confirm the assumption that in the horse, as in other mammalian species, casein genes are organized in a cluster located on a single chromosome, we performed in situ hybridization of pro-metaphase chromosomes with two BAC clones containing different equine casein genes. Sequence analysis of the β-casein and κ-casein gene proximal promoters revealed binding sites for activators (STAT5, GRE, NF1, MAF) and repressors (YY1, PMF), characteristic for casein genes. The alignments of casein gene promoters revealed the highest sequence identity in the proximal promoter region between the equine and human β-casein gene promoters. We directly compared the activity of equine β-casein and κ-casein gene promoters in vitro using bovine mammary gland cell line BME-UV1. In this system, the κ-casein gene proximal promoter activated the reporter gene expression more efficiently than the β-casein gene promoter of approximately the same length. The 810 bp of β-casein promoter activated the reporter gene expression more efficiently than the long fragment (1920 bp) and the 1206 bp fragment of the same promoter, which included also 396 bp of 5′ UTR.

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Journal of Dairy Research
  • ISSN: 0022-0299
  • EISSN: 1469-7629
  • URL: /core/journals/journal-of-dairy-research
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