Hostname: page-component-848d4c4894-wg55d Total loading time: 0 Render date: 2024-06-02T10:48:18.177Z Has data issue: false hasContentIssue false
  • English
  • Français

Hormonal control of casein synthesis in organ culture of the bovine lactating mammary gland

Published online by Cambridge University Press:  01 June 2009

Arieh Gertler ,
Anat Weil  and
Nava Cohen
Arieh Gertler
Affiliation:
The Hebrew University of Jerusalem, Faculty of Agriculture, Department of Agricultural Biochemistry, Rehovot 76–100, Israel
Anat Weil
Affiliation:
The Hebrew University of Jerusalem, Faculty of Agriculture, Department of Agricultural Biochemistry, Rehovot 76–100, Israel
Nava Cohen
Affiliation:
The Hebrew University of Jerusalem, Faculty of Agriculture, Department of Agricultural Biochemistry, Rehovot 76–100, Israel
Get access Rights & Permissions [Opens in a new window]

Summary

Explants from lactating bovine mammary gland were cultured in vitro in serum-free medium through 1–9 d. Casein synthesis was determined by [32P] incorporation into newly synthesized Ca rennin precipitable fraction. High correlation (γ = 0·98) was found between incorporation of [32P] and [3H]amino acids in explants cultured under different hormonal regimes, thus indicating that post-translational phosphorylation is not a rate-limiting step in casein synthesis.

Hormonal effects on casein synthesis were studied by supplementing the incubating medium with insulin (I), prolactin (PRL), cortisol (F), thyroxine (T4) and triiodothyronine (T3). It was found that both PRL and I were required absolutely for maximal synthesis and almost maximal effect was achieved with 50 ng/ml. The effect of F was less clear, but some increase was achieved at the 200–1000 ng/ml range. T4 and T3 did not affect casein synthesis at a range of 10-11–10-7M while a significant inhibition was observed at 2 × 10-5 M. A time-course study of casein synthesis further substantiated the dominant role of PRL in maintenance or even elevation of the initial rate of casein synthesis in the explants, through the first 4 d of incubation.

Type
Original Articles
Information
Journal of Dairy Research , Volume 49 , Issue 3 , August 1982 , pp. 387 - 398
Copyright
Copyright © Proprietors of Journal of Dairy Research 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Andersen, C. R. & Larson, B. L. 1970 Comparative maintenance of function in dispersed cell and organ cultures of bovine mammary tissue. Experimental Cell Research 61 24–30.CrossRefGoogle ScholarPubMed
Beeby, R. & Mocquot, G. 1969 The sensitivity to rennin of an acid-soluble fraction of casein. Biochimica et Biophysica Acta 175 430–433.CrossRefGoogle ScholarPubMed
Bines, J. A., Hart, I. C. & Morant, S. V. 1980 Endocrine control of energy metabolism in the cow: the effect on milk yield and levels of some blood constituents of injecting growth hormone and growth hormone fragments. British Journal of Nutrition 43 179–188.CrossRefGoogle ScholarPubMed
Bolander, F. F., Nicholas, K. R. & Topper, Y. J. 1979 Retention of glucocorticoid by isolated mammary tissue may complicate interpretation of results from in vitro experiments. Biochemical and Biophysical Research Communications 91 247–252.CrossRefGoogle ScholarPubMed
Buttle, H. L., Cowie, A. T., Jones, E. A. & Turvey, A. 1979 Mammary growth during pregnancy in hypophysectomized or bromocriptine-treated goats. Journal of Endocrinology 80 343–351.CrossRefGoogle ScholarPubMed
Canales, E. S., Garrido, J. T., Zarate, A., Mason, M. & Soria, J. 1976 Effect of ergonovine on prolactin secretion and milk let-down. Obstetrics and Gynecology 48 228–235.Google ScholarPubMed
Collier, R. J., Bauman, D. E. & Hays, R. L. 1977 Lactogenesis in explant cultures of mammary tissue from pregnant cows. Endocrinology 100 1192–1200.Google Scholar
Djiane, J., Delouis, C. & Denamur, R. 1975 Lactogenesis in organ Cultures of heifer mammary tissue. Journal of Endocrinology 65 453–454.CrossRefGoogle ScholarPubMed
Forsyth, I. A. 1971 Reviews of the progress of dairy science. Section A. Physiology. Organ culture techniques and the study of hormone effects on the mammary gland. Journal of Dairy Research 38 419–444.CrossRefGoogle Scholar
Flückiger, E. & Wagner, H. R. 1968 [2-Br-±-ergocriptine's effect on fertility and lactation in the rat] Experientia 24 1130–1131.CrossRefGoogle Scholar
Ganguly, R., Ganguly, N., Mehta, N. M. & Banerjee, M. R. 1980 Absolute requirement of glucocorticoid for expression of the casein gene in the presence of prolactin. Proceedings, National Academy of Sciences of the U.S.A. 77 6003–6006.CrossRefGoogle ScholarPubMed
Gorewit, R. C. & Tucker, H. A. 1976 Glucocorticoid binding in mammary tissue slices of cattle in various reproductive states. Journal of Dairy Science 59 1890–1896.CrossRefGoogle ScholarPubMed
Gorski, J. 1979 Endocrine factors in genetic improvement of milk production. Journal of Dairy Science 62 814–817CrossRefGoogle ScholarPubMed
Guyette, W. A., Matusik, R. J. & Rosen, J. M. 1979 Prolactin-mediated transcriptional and post-transcriptional control of casein gene expression. Cell 17 1013–1023.CrossRefGoogle ScholarPubMed
Hart, I. C. 1976 Prolactin, growth hormone, insulin and thyroxine: their possible roles in steroid-induced mammary growth and lactation in the goat. Journal of Endocrinology 71 41P–42P.Google Scholar
Hooley, R. D., Campbell, J. J. & Findlay, J. K. 1978 The importance of prolactin for lactation in the ewe. Journal of Endocrinology 79 301–310.Google Scholar
Hooley, R. D., Fell, L. R. & Findlay, J. K. 1977 The effect of 2-bromo-α-ergocryptine and 2-chloro-α-methylergoline-8β-acetonitrile (lergotrile mesylate) on the prolactin secretion of the ewe. Acta Endocrinologica, 86 722–727.Google ScholarPubMed
Houdebine, L. M. 1976 Effects of prolactin and progesterone on expression of casein genes: titration of casein mRNA by hybridization with complementary DNA. European Journal of Biochemistry 68 219–225.Google Scholar
Houdebine, L. M., Delouis, C. & Devinoy, E. 1978 b Post-transcriptional stimulation of casein synthesis by thyroid hormone. Biochimie 60 809–812CrossRefGoogle ScholarPubMed
Houdebine, L. M., Devinoy, E. & Delouis, C. 1978 a Stabilization of casein mRNA by prolactin and glucocorticoids. Biochimie 60 57–63.CrossRefGoogle ScholarPubMed
Johke, T. & Hodate, K. 1978 Effects of CB154 on serum hormone level and lactogenesis in dairy cows. Endocrinologica Japonica 25 67–74.CrossRefGoogle ScholarPubMed
Juergens, W. G., Stockdale, F. E., Topper, Y. J. & Elias, J. J. 1965 Hormone-dependent differentiation of mammary gland in vitro. Proceedings, National Academy of Sciences of the U.S.A. 54 629–634.CrossRefGoogle ScholarPubMed
Kann, G. 1976 Inhibition of prolactin secretion in the ewe by 2-Br-ergocriptin during pregnancy or early lactation: effect on milk yield. Proceedings Vth International Congress of Endocrinology p. 251.Google Scholar
Karg, H., Sohams, D. & Reinhardt, V. 1972 Effects of 2-Br-α-ergocriptine on plasma prolactin level and milk yield in cows. Experientia 28 574–576Google Scholar
Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680–685.Google Scholar
Matusik, R. J. & Rosen, J. M. 1978 Prolactin induction of casein mRNA in organ culture: a model system for studying peptide hormone regulation of gene expression. Journal of Biological Chemistry 253 2343–2347.CrossRefGoogle Scholar
Mills, E. S. & Topper, Y. J. 1970 Some ultrastructural effects of insulin, hydrocortisone, and prolactin on mammary gland explants. Journal of Cell Biology 44 310–328.Google Scholar
Mullin, W. J. & Wolfe, F. H. 1974 Disc gel electrophoresis of caseins treated with proteolytic and glycolytic enzymes. Journal of Dairy Science 57 9–14CrossRefGoogle ScholarPubMed
Nagaiah, K., Bolander, F. F., Nicholas, K. R., Takemoto, T. & Topper, Y. J. 1981 Prolactin-induced accumulation of casein mRNA in mouse mammary explants: a selective role of glucocorticoid. Biochemical and Biophysical Research Communications 98 380–387Google Scholar
Nickerson, S. C., Heald, C. W., Bibb, T. L. & McGilliard, M. L. 1978 Cytological effects of hormones and plasma on bovine mammary tissue in vitro. Journal of Endocrinology 79 363–368.CrossRefGoogle ScholarPubMed
Peters, F., Lummerich, M. & Breckwoldt, M. 1979 Inhibition of prolactin and lactation by methylergometrine hydrogenmaleate. Acta Endocrinologica 91 213–216.Google Scholar
Schams, D., Reinhardt, V. & Karg, H. 1972 Effects of 2-Br-α-ergocriptine on plasma prolactin level during parturition and onset of lactation in cows. Experientia 28 697–699.CrossRefGoogle Scholar
Topper, Y. J. & Freeman, C. S. 1980 Multiple hormone interactions in the developmental biology of the mammary gland. Physiological Reviews 60 1049–1106.Google Scholar
Topper, Y. J., Oka, T. & Vonderhaar, B. K. 1975 Techniques for studying development of normal mammary epithelial cells in organ culture. Methods in Enzymology 39 443–454CrossRefGoogle ScholarPubMed
Vonderhaar, B. K. 1977 Studies on the mechanism by which thyroid hormones enhance α-lactalbumin activity in explants from mouse mammary glands. Endocrinology 100 1423–1431.Google Scholar
Weil, A., Cohen, N. & Gertler, A. 1981 Hormonal control of casein synthesis in organ culture of bovine mammary tissue. Israeli Journal of Medical Sciences (Abstr.) 17 479Google Scholar