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Hormone-dependent expression of the ovine β-lactoglobulin gene

  • Rebecca Osborne (a1) (a2), Michael Howell (a1) (a2), A. John Clark (a1) (a3) and Kevin R. Nicholas (a1)

Summary

The minimal hormonal requirements for inducing the ovine β-lactoglobulin gene have been investigated using mammary gland explants from ewes in the first half of pregnancy. Quantification of β-lactoglobulin mRNA showed that a combination of insulin, Cortisol and prolactin was required to stimulate the expression of the gene and that this response could not be enhanced by the addition of oestrogen and thyroid hormone to the culture medium. Explants cultured in the presence of insulin, Cortisol and prolactin also demonstrated the capacity to synthesize the protein. Progesterone did not inhibit the induction of the gene, which is consistent with the increase in β-lactoglobulin mRNA observed in vivo in the mammary gland during the final 2 months of pregnancy when the circulating level of progesterone is elevated.

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Anderson, R. R. 1975 Mammary gland growth in sheep. Journal of Animal Science 41 118123
Archibald, A. L., Mcclenaghan, M., Hornsey, V., Simons, J. P. & Clark, A. J. 1990 High-level expression of biologically active human αt-antitrypsin in the milk of transgenic mice. Proceedings of the National Academy of Sciences, USA 87 51785182
Bassett, J. M., Oxborrow, T. J., Smith, I. D. & Thorburn, G. B. 1969 The concentration of progesterone in the peripheral plasma of the pregnant ewe. Journal of Endocrinology 45 449457
Bolander, F. F. & Topper, Y. J. 1980 a Stimulation of lactose synthetase activity and casein synthesis in mouse mammary explants by estradiol. Endocrinology 106 490495
Bolander, F. F. & Topper, Y. J. 1980 b Loss of differentiative potential of the mammary gland in ovariectomized mice: detection and reversibility of the defect. Endocrinology 107 12811285
Bolander, F. F. & Topper, Y. J. 1981 Loss of differentiative potential of the mammary gland in ovariectomized mice: identification of a biochemical lesion. Endocrinology 108 16491653
Chomczynski, P. & Sacchi, N. 1987 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162 156159
Collet, C., Joseph, R. J. & Nicholas, K. R. 1990 Cloning, cDNA analysis and prolactin-dependent expression of a marsupial α-lactalbumin. Reproduction, Fertility and Development 2 693701
Collet, C., Joseph, R. J. & Nicholas, K. R. 1991 A marsupial β-lactoglobulin gene: characterisation and prolactin-dependent expression. Journal of Molecular Endocrinology 6 916
Dodd, S. C., Forsyth, I. A., Buttle, H. L., Gurr, M. I. & Dils, R. R. 1994 Hormonal induction of α-lactalbumin and β-lactoglobulin in cultured mammary explants from pregnant pigs. Journal of Dairy Research 61 3545
Gaye, P., Hue-Delahaie, D., Mercier, J. -C, Soulier, S., Vilotte, J. -L. & Furet, J. -P. 1986 Ovine β-lactoglobulin messenger RNA. Nucleotide sequence and mRNA levels during functional differentiation of the mammary gland. Biochimie 68 10971107
Harris, S., Mcclenaghan, M., Simons, J. P., Ali, S. & Clark, A. J. 1991 Developmental regulation of the sheep β-lactoglobulin gene in the mammary gland of transgenic mice. Developmental Genetics 12 299307
Hartmann, P. E., Trevethan, P. & Shelton, J. N. 1973 Progesterone and oestrogen and the initiation of lactation in ewes. Journal of Endocrinology 59 249259
Houdebine, L. -M., Delouis, C. & Devinoy, E. 1978 Post-transcriptional stimulation of casein synthesis by thyroid hormone. Biochimie 60 809812
Houdebine, L. -M., Djiane, J., Dusanter-Fourt, I., Martel, P., Kelly, P. A., Devinoy, E. & Servely, J. -L. 1985 Hormonal action controlling mammary activity. Journal of Dairy Science 68 489500
Kuhn, N. J. 1977 Lactogenesis: the search for trigger mechanisms in different species. In Comparative Aspects of Lactation, pp. 165192 (Ed. Peaker, M.). London: Academic Press
Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680685
Lesueur, L., Edery, M., Ali, S., Paly, J., Kelly, P. A. & Djiane, J. 1991 Comparison of long and short forms of the prolactin receptor on prolactin-induced milk protein gene transcription. Proceedings of the National Academy of Sciences, USA 88 824828
Lesueur, L., Edery, M., Paly, J., Clark, J., Kelly, P. A. & Djiane, J. 1990 Prolactin stimulates milk protein promoter in CHO cells cotransfected with prolactin receptor cDNA. Molecular and Cellular Endocrinology 71 R7R12
Maher, F. & Nicholas, K. R. 1987 Pituitary-induced lactation in mammary gland explants from the pregnant tammar (Macropus eugenii): a negative role for cyclic AMP. Comparative Biochemistry and Physiology 87A 11071117
Nicholas, K. R., Sankaran, L. & Topper, Y. J. 1981 The induction of α-lactalbumin in rat mammary explants in the absence of exogenous prolactin: effects of progesterone and estrogen. Endocrinology 109 978980
Nicholas, K. R. & Topper, Y. J. 1980 Enhancement of α-laetalbumin-like activity in mammary explants from pregnant rats in the absence of exogenous prolactin. Biochemical and Biophysical Research Communications 94 14241431
Nicholas, K. R. & Tyndale-Biscoe, C. H. 1985 Prolactin-dependent accumulation of β-lactalbumin in mammary gland explants from the pregnant tammar wallaby (Macropus eugenii). Journal of Endocrinology 106 337342
Puissant, C., Attal, J. & Houdebine, L. M. 1990 The hormonal control of ovine β-lactoglobulin gene in cultured mammary explants from ewes. Reproduction, Nutrition, Développement 30 245251
Rosen, J. M., Poyet, P., Goodman, H. & Lee, K. -F. 1989 Mechanisms by which prolactin and glucocorticoids regulate casein gene expression. In Gene Expression: Regulation at the RNA and Protein Levels, pp. 115123 (Eds Kay, J., Ballard, F. J. and Mayer, R. J.). London: The Biochemical Society (Biochemical Society Symposium no. 55)
Shamay, A., Pursel, V. G., Wall, R. J. & Hennighausen, L. 1992 Induction of lactogenesis in transgenic virgin pigs: evidence for gene and integration site-specific hormonal regulation. Molecular Endocrinology 6 191197
Terada, N. & Oka, T. 1982 Selective stimulation of α-lactalbumin synthesis and its mRNA accumulation by thyroid hormone in the differentiation of the mouse mammary gland in vitro. FEBS Letters 149 101104
Topper, Y. J. & Freeman, C. S. 1980 Multiple hormone interactions in the developmental biology of the mammary gland. Physiological Reviews 60 10491106
Topper, Y. J., Oka, T. & Vonderhaar, B. K. 1975 Techniques for studying development of normal mammary epithelial cells in organ culture. Methods in Enzymology 39 443454
Vonderhaar, B. K. 1977 Studies on the mechanism by which thyroid hormones enhance α-lactalbumin activity in explants from mouse mammary glands. Endocrinology 100 14231431
Vonderhaar, B. K. & Ziska, S. E. 1989 Hormonal regulation of milk protein gene expression. Annual Review of Physiology 51 641652
Warner, B., Janssens, P. A. & Nicholas, K. R. 1993 Prolactin-independent induction of a-lactalbumin gene expression in mammary gland explants from pregnant Balb/c mice. Biochemical and Biophysical Research Communications 194 987991
Watson, C. J., Gordon, K. E., Robertson, M. & Clahk, A. J. 1991 Interaction of DNA-binding proteins with a milk protein gene promoter in vitro: identification of a mammary gland-specific factor. Nucleic Acids Research 19 66036610
Wright, G., Carver, A., Cottom, D., Reeves, D., Scott, A., Simons, P., Wilmut, I. & Colman, A. 1991 High-level expression of active human α-antitrypsin in the milk of transgenic sheep. Biotechnology 9 830834
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Journal of Dairy Research
  • ISSN: 0022-0299
  • EISSN: 1469-7629
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