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In vitro characterization of anti-inflammatory activities of 3RS, 7R, 11R-phytanic acid

Published online by Cambridge University Press:  23 February 2023

Tomonori Nakanishi ,
Mikihisa Izumi ,
Ryoji Suzuki ,
Kohta Yamaguchi ,
Kazuhiro Sugamoto ,
Laurie Erickson  and
Satoshi Kawahara
Tomonori Nakanishi
Affiliation:
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan
Mikihisa Izumi
Affiliation:
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan
Ryoji Suzuki
Affiliation:
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan
Kohta Yamaguchi
Affiliation:
Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Miyazaki, Japan
Kazuhiro Sugamoto
Affiliation:
Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Miyazaki, Japan
Laurie Erickson
Affiliation:
Department of Biology, Harold Washington City College of Chicago, Chicago, IL, USA Department of Health Sciences, Blitstein Institute of Hebrew Theological College, Chicago, IL, USA
Satoshi Kawahara*
Affiliation:
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan
*
Author for correspondence: Satoshi Kawahara, Email: animalproducts@cc.miyazaki-u.ac.jp
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Abstract

The aim of the research described here was to investigate the in vitro immunomodulatory effects of 3RS, 7R, 11R-phytanic acid (3RS-PHY) from the perspective of efficacy against autoimmune diseases. 3RS-PHY is a milk component with strong agonist activity at the peroxisome proliferator activated receptor (PPAR). As PPAR is a therapeutic target for several human diseases, 3RS-PHY intake may have possible health benefits. Recently, we chemically synthesized a preparation of 3RS-PHY and demonstrated that 3RS-PHY inhibited T-cell production of interferon (IFN)-γ. However, the overall immunomodulatory effects were not evaluated. In this study, mouse splenocytes, purified T-cells and B-cells were stimulated by mitogens and incubated with 3RS-PHY, followed by evaluation of cytokine and antibody production. A macrophage-like cell line J774.1 was also incubated with 3RS-PHY to evaluate nitric oxide production. 3RS-PHY decreased mRNA levels not only of IFN-γ but also of interleukin (IL)-2, IL-10 and IL-17A in splenocytes and similar effects were confirmed at the protein level. In addition, 3RS-PHY had a direct action on T-cells with preferential inhibitory effects on Th1 and Th17 cytokines such as IFN-γ and IL-17A. Furthermore, 3RS-PHY suppressed antibody secretion by B-cells and nitric oxide production by J774.1 almost completely, indicating that 3RS-PHY is a bioactive fatty acid with anti-inflammatory properties. These findings encourage further investigations, including in vivo experiments, to evaluate whether 3RS-PHY actually shows the potential to prevent autoimmune diseases, and provide basic information to produce milk and dairy products with an increased 3RS-PHY concentration.

Keywords

3RS, 7R, 11R-phytanic acidantibodycytokinenitric oxide
Type
Research Article
Information
Journal of Dairy Research , Volume 90 , Issue 1 , February 2023 , pp. 92 - 99
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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