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Influence of milk processing temperature on growth performance, nitrogen retention, and hindgut's inflammatory status and bacterial populations in a calf model

  • Alex Bach (a1) (a2), Anna Aris (a2), Maria Vidal (a2), Francesc Fàbregas (a2) and Marta Terré (a2)...

Abstract

This research communication describes a study aimed at evaluating the effects of heat treatment of milk on growth performance, N retention, and hindgut's inflammatory status and bacterial populations using young dairy calves as a model. Twenty-one Holstein calves were randomly allocated to one of three treatments: raw milk (RM), pasteurised milk (PAST), or UHT milk (UHT). Calves were submitted to a N balance study, and a biopsy from the distal colon and a faecal sample were obtained from 5 animals per treatment to determine expression of several genes and potential changes in the hindgut's bacterial population. Milk furosine content was 33-fold greater in UHT than in RM and PAST milks. Calves receiving RM grew more than those fed UHT, and urinary N excretion was greatest in calves fed UHT. Quantification of Lactobacillus was lower in calves consuming PAST or UHT, and Gram negative bacteria were greater in UHT than in PAST calves. The expression of IL-8 in the hindgut's mucosa was lowest and that of IL-10 tended to be lowest in RM calves, and expression of claudin-4 tended to be greatest in UHT calves. In conclusion, the nutritional value of UHT-treated milk may be hampered because it compromises growth and increases N excretion in young calves and may have deleterious effects on the gut's bacterial population and inflammation status.

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Corresponding author

*For correspondence; e-mail: alex.bach@icrea.cat

References

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Allen, MS, Bradford, BJ & Harvatine, KJ 2005 The cow as a model to study food intake regulation. Annual Review of Nutrition 25 523547
Al-Saadi, JMS, Easa, AM & Deeth, HC 2012 Effect of lactose on cross-linking of milk proteins during heat treatments. International Journal of Dairy Technology 66 16
Amador-Espejo, GG, Suàrez-Berencia, A, Juan, B, Bárcenas, ME & Trujillo, AJ 2014 Effect of moderate inlet temperatures in ultra-high-pressure homogenization treatments on physicochemical and sensory characteristics of milk. Journal of Dairy Science 97 659671
AOAC 2000 Official Methods of Analysts 17th edition, vol. I. Arlington, VA: AOAC International
Asseman, C, Mauze, S, Leach, MW, Coffman, RL & Powrie, F 1999 An essential role for interleukin 10 in the function of regulatory t cells that inhibit intestinal inflammation. Journal of Experimental Medicine 190 9951004
Claeys, WL, Verraes, C, Cardoen, S, De Block, J, Huyghebaert, A, Raes, K, Dewettinck, K & Herman, L 2014 Consumption of raw or heated milk from different species: An evaluation of the nutritional and potential health benefits. Food Control 42 188201
Elmadfa, I, Klein, P & Meyer, AL 2010 Immune-stimulating effects of lactic acid bacteria in vivo and in vitro. Proceedings of the Nutrition Society 69 416420
Friedman, M 1996 Food browning and its prevention: an overview. Journal of Agriculture and Food Chemistry 44 631653
IDF 2004 International Dairy Federation. Milk and Milk Products – Determination of Furosine Content – Ion-Pair Reverse-Phase High-Performance Liquid Chromatography Method. Brussels, Belgium: IDF
IDF 2010 International Dairy Federation. Milk – Determination of Fat Content – Gravimetric Method (Reference Method). Brussels, Belgium: IDF
Lacroix, M, Bos, C, Léonil, J, Airinei, G, Luengo, C, Daré, S, Benamouzig, R, Fouillet, H, Fauquant, J, Tomé, D & Gaudichon, C 2006 Compared with casein or total milk protein, digestion of milk soluble proteins is too rapid to sustain the anabolic postprandial amino acid requirement. American Journal of Clinical Nutrition 84 10701079
Lacroix, M, Bon, C, Bos, C, Leonil, J, Benamouzig, R, Luengo, C, Fauquant, J, Tomé, D & Gaudichon, C 2008 Ultra high temperature treatment, but not pasteurization, affects the postprandial kinetics of milk proteins in humans. Journal of Nutrition 138 23422347
Li, Y, Ostergaard, MV, Jiang, P, Chatterton, DEW, Thymann, T, Kvistgaard, AS & Sanglid, PT 2013 Whey protein processing influences formula-induced gut maturation in preterm pigs. Journal of Nutrition 143 19341942
Maes, M, Kubera, M & Leunis, JC 2008 The gut-brain barrier in major depression: intestinal mucosal dysfunction with an increased translocation of LPS from gram negative enterobacteria (leaky gut) plays a role in the inflammatory pathophysiology of depression. Neuroendocrinology Letters 29 117124
Mao, S, Zhang, M, Liu, J & Zhu, W 2015 Characterising the bacterial microbiota across the gastrointestinal tracts of dairy cattle: membership and potential function. Scientific Reports 5 16116
Mazzucchelli, L, Hauser, C & Zgraggen, Z 1994 Expression of interleukin-8 gene in inflammatory bowel disease is related to the histological grade of active inflammation. American Journal of Pathology 144 9971007
Nicorescu, I, Riaublanc, A, Loisel, C, Vial, C, Djelveh, G, Cuvelier, G & Legrand, J 2009 Impact of protein self-assemblages on foam properties. Food Research International 42 14341445
NRC National Research Council 2001 Nutrient Requirements of Dairy Cattle. 7th revised edition. Washington, DC: National Academy of Science
Oikonomou, G, Teixeira, A, Foditsch, C & Bicalho, ML 2013 Fecal microbial diversity in pre-weaned dairy calves as described by pyrosequencing of metagenomic 16S rDNA. Associations of Faecalibacterium species with health and growth. PLoS ONE 8 e63157
Pfaf, MW 2014 Quantification strategies in real-time PCR. In A–Z of Quantitative PCR, pp. 87112 (Ed. Bustin, SA). La Jolla, CA: International University Line
Povoa, M & Moraes-Santos, T 1997 Effect of heat treatment on the nutritional quality of milk proteins. International Dairy Journal 7 609612
Rerat, A, Calmes, R, Vaissade, P & Finot, PA 2002 Nutritional and metabolic consequences of the early Maillard reaction of heat treated milk in the pig. Significance for man. European Journal of Nutrition 41 111
Terré, M, Devant, M & Bach, A 2007 Effect of level of milk replacer fed to Holstein calves on performance during the preweaning period and starter digestibility at weaning. Livestock Science 110 8288
Van Boekel, M 1998 Effect of heating on Maillard reactions in milk. Food Chemistry 62 403414
Wada, Y & Lönnerdal, B 2014 Effects of different industrial heating processes of milk on site-specific protein modifications and their relationship to in vitro and in vivo digestibility. Journal of Agriculture and Food Chemistry 62 41754185

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