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Interactions of temperate bacteriophages of Streptococcus salivarius subsp. thermophilus with lysogenic indicators affect phage DNA restriction patterns and host ranges

Published online by Cambridge University Press:  01 June 2009

Blandine Fayard ,
Moritz Haefliger  and
Jean-Pierre Accolas
Blandine Fayard
Affiliation:
Station de Recherches Laitières, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France
Moritz Haefliger
Affiliation:
Station de Recherches Laitières, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France
Jean-Pierre Accolas
Affiliation:
Station de Recherches Laitières, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France
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Summary

After treatment with mitomycin C, 12 of the 120 strains of the French Collection of Lactic Acid Bacteria (CNRZ) belonging to the species Streptococcus salivarius subsp. thermophilus were found to be inducible and lysogenic. The corresponding temperate phages were multiplied on indicator strains and further characterized. It is noteworthy that some of the indicators were themselves lysogenic. The temperate phages belonged to Bradley's group B, or to the Siphoviridae family of the International Committee on Taxonomy of Viruses. Seven of them were shared among the two protein profiles previously established for virulent phages of Str. salivarius subsp. thermophilus. Seven different DNA restriction profiles were found for ten native temperate phages examined just after mitomycin C induction. Genome sizes varied from 40 to 45 kb and were classified into four related homology groups by DNA cross-hybridization, but there was no clear-cut relationship with the protein clusters previously shown. DNA homology with representatives of virulent phages was also found. The DNA restriction profiles of seven native temperate phages, examined just after mitomycin C induction of the lysogenic hosts, were noticeably different from those of the corresponding phages once they had been propagated on lysogenic indicators. Moreover, the host range of the latter phages was extended to a greater number of sensitive strains. The possible role of lysogenic strains of Str. salivarius subsp. thermophilus in the occurrence of phage outbreaks affecting this dairy streptococcal species is discussed.

Type
Original Articles
Information
Journal of Dairy Research , Volume 60 , Issue 3 , August 1993 , pp. 385 - 399
Copyright
Copyright © Proprietors of Journal of Dairy Research 1993

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References

REFERENCES

Accolas, J.-P. & Spillmann, H. 1979 The morphology of six bacteriophages of Streptococcus thermophilus. Journal of Applied Bacteriology 47 135–144CrossRefGoogle Scholar
Auclair, J. & Accolas, J.-P. 1983 Use of thermophilic lactic starters in the dairy industry. Antonie van Leeuwenhoek Journal of Microbiology 49 313–326CrossRefGoogle ScholarPubMed
Benbadis, L., Faelen, M., Slos, P., Fazel, A. & Mercenier, A. 1990 Characterization and comparison of virulent bacteriophages of Streptococcus thermophilus isolated from yogurt. Biochimie 72 855–862CrossRefGoogle ScholarPubMed
Bradley, D. E. 1967 Ultrastructure of bacteriophages and bacteriocins. Bacteriological Reviews 31 230–314CrossRefGoogle ScholarPubMed
Carminati, D. & Giraffa, G. 1992 Evidence and characterization of temperate bacteriophage in Streptococcus salivarius subsp. thermophilus St18. Journal of Dairy Research 59 71–79CrossRefGoogle ScholarPubMed
Ciblis, E. 1970 [Characterization of a bacteriophage of Streptococcus thermophilus.] Zentralblatt für Bakteriologie Parasitenkunde, Infektionkrankheiten und Hygiene Abt. II 125 541–554Google ScholarPubMed
Cluzel, P.-J., Veaux, M., Rousseau, M. & Accolas, J.-P. 1987 Evidence for temperate bacteriophages in two strains of Lactobacillus bulgaricus. Journal of Dairy Research 54 397–405CrossRefGoogle ScholarPubMed
Colmin, C., Pebay, M., Simonet, J. M. & Decaris, B. 1991 A species-specific DNA probe obtained from Streptococcus salivarius subsp. thermophilus detects strain restriction polymorphism. FEMS Microbiology Letters 81 123–128CrossRefGoogle Scholar
Deane, D. D., Nelson, F. E., Ryser, F. C. & Carr, P. H. 1953 Streptococcus thermophilus bacteriophage from Swiss cheese whey. Journal of Dairy Science 36 185–191CrossRefGoogle Scholar
Elliker, P. R., Anderson, A. W. & Hannesson, G. 1956 An agar culture medium for lactic acid streptococci and lactobacilli. Journal of Dairy Science 39 1611–1612CrossRefGoogle Scholar
Francki, R. I. B., Fauquet, C. M., Knudson, D. L. & Brown, F. (Eds) 1991 Classification and nomenclature of viruses. Fifth report of the International Committee on Taxonomy of Viruses. Archives of Virology (Suppl. 2) 1–450CrossRefGoogle Scholar
Heap, H. A. & Lawrence, R. C. 1988 Culture systems for the dairy industry. In Developments in Food Microbiology—4, pp. 149–185 (Ed. Robinson, R. K.). London: Elsevier Applied ScienceCrossRefGoogle Scholar
Kiuru, V. J. T. & Tybeck, E. 1955 Characteristics of bacteriophages active against lactic acid bacteria in Swiss cheese. Suomen Kemistilehti B 28 57–62Google Scholar
Krusch, U., Neve, H., Luschei, B. & Teuber, M. 1987 [Characterization of virulent bacteriophages of Streptococcus salivarius subsp. thermophilus by host specificity and electron microscopy] Kieler Milchwirtschaftliche Forschungsberichte 39 155–167Google Scholar
Kurmann, J. L. 1979 [Acidification defects caused by bacteriophages in hard-cheese factories.] Schweizerische Milchwirtschaftliche Forschung 8 71–76Google Scholar
Kurmann, J. L. 1983 [Liberation by penicillin of bacteriophages from thermophilic lactic acid bacteria, a hidden cause of phage-induced acidification problems.] Schweizerische Milchwirtschaftliche Forschung 12 39–43Google Scholar
Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680–685CrossRefGoogle ScholarPubMed
Larbi, D., Colmin, C., Roussellle, L., Decaris, B. & Simonet, J. M. 1990 Genetic and biological characterization of nine Streptococcus salivarius subsp. thermophilus bacteriophages. Lait 70 107–116CrossRefGoogle Scholar
Marmur, J. 1961 A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3 208–213CrossRefGoogle Scholar
Mercenier, A. 1990 Molecular genetics of Streptococcus thermophilus. FEMS Microbiology Reviews 87 61–77CrossRefGoogle Scholar
Neve, H., Krusch, U. & Teuber, M. 1989 Classification of virulent bacteriophages of Streptococcus salivarius subsp. thermophilus isolated from yoghurt and Swiss-type cheese. Applied Microbiology and Biotechnology 30 624–629CrossRefGoogle Scholar
Neve, H., Krusch, U. & Teuber, M. 1990 Virulent and temperate bacteriophages of thermophilic lactic acid streptococci. FEMS Microbiology Reviews 87 P58Google Scholar
Prèvots, F., Relano, P., Mata, M. & Ritzenthaler, P. 1989 Close relationship of virulent bacteriophages of Streptococcus salivarius subsp. thermophilus at both the protein and the DNA level. Journal of General Microbiology 135 3337–3344Google Scholar
Séchaud, L., Rousseau, M., Fayard, B., Callegari, M. L., Quénée, P. & Accolas, J.-P. 1992 Comparative study of 35 bacteriophages of Lactobacillus helveticus: Morphology and host range. Applied and Environmental Microbiology 58 1011–1018CrossRefGoogle ScholarPubMed
Smaczny, T. 1983 [Acidification disturbance in manufacture of yogurt and Bioghurt caused by bacteriophages and bacteriocins.] Thesis, Rheinischen Friedrich-Wilhelms-Universität, Bonn, GermanyGoogle Scholar
Smaczny, T. & Krämer, J. 1984 [Acidification disturbance in manufacture of yogurt, Bioghurt® and Biogarde® caused by bacteriocins and bacteriophages of Streptococcus thermophilus. II. Distribution and characterization of bacteriophages.] Deutsche Molkerei-Zeitung 105 614–618Google Scholar
Terzaghi, B. E. & Sandine, W. E. 1975 Improved medium for lactic streptococci and their bacteriophages. Applied Microbiology 29 807–813CrossRefGoogle ScholarPubMed
Yamamoto, K. R., Alberts, B. M., Benzinger, R., Lawhorne, L. & Treiber, G. 1970 Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40 734–744CrossRefGoogle ScholarPubMed
Zourari, A. 1991 [Characterization of thermophilic lactic acid bacteria isolated from Greek traditional yogurts.] Doctoral Thesis, Institut National Agronomique, Paris-Grignon, FranceGoogle Scholar
Zourari, A., Accolas, J.-P. & Desmazeaud, M. J. 1992 Metabolism and biochemical characteristics of yogurt bacteria. A review. Lait 72 1–33CrossRefGoogle Scholar