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    Pan, Maomao Shen, Shaochuan Chen, Liang Dai, Bin Xu, Linhong Yun, Junxian Yao, Kejian Lin, Dong-Qiang and Yao, Shan-Jing 2015. Separation of lactoperoxidase from bovine whey milk by cation exchange composite cryogel embedded macroporous cellulose beads. Separation and Purification Technology, Vol. 147, p. 132.

    2012. Biotechnology of Plasma Proteins.


Isolation of lactoperoxidase using different cation exchange resins by batch and column procedures

  • Leonard WT Fweja (a1), Michael J Lewis (a1) and Alistair S Grandison (a1)
  • DOI:
  • Published online: 19 May 2010

Lactoperoxidase (LP) was isolated from whey protein by cation-exchange using Carboxymethyl resin (CM-25C) and Sulphopropyl Toyopearl resin (SP-650C). Both batch and column procedures were employed and the adsorption capacities and extraction efficiencies were compared. The resin bed volume to whey volume ratios were 0·96:1·0 for CM-25C and ⩽0·64:1·0 for SP-650 indicating higher adsorption capacity of SP-650 compared with CM-25C. The effluent LP activity depended on both the enzyme activity in the whey and the amount of whey loaded on the column within the saturation limits of the resin. The percentage recovery was high below the saturation point and fell off rapidly with over-saturation. While effective recovery was achieved with column extraction procedures, the recovery was poor in batch procedures. The whey-resin contact time had little impact on the enzyme adsorption. SDS PAGE and HPLC analyses were also carried out, the purity was examined and the proteins characterised in terms of molecular weights. Reversed phase HPLC provided clear distinction of the LP and lactoferrin (LF) peaks. The enzyme purity was higher in column effluents compared with batch effluents, judged on the basis of the clarity of the gel bands and the resolved peaks in HPLC chromatograms.

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E Casal , A Montilla , FJ Moreno , A Olano & N Corzo 2006 Use of chitosan for selective removal of beta lactoglobulin from whey. Journal of Dairy Science 89 13841389

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KGH Paul , PI Ohlsson & A Henriksson 1980 The Isolation and some ligandic properties of lactoperoxidase. FEBS Letters 110 200204

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S Yoshida 1988 Isolation of some minor proteins distributed in acid whey from approximately 100,000 to 250,000 Daltons of particle size. Journal of Dairy Science 71 19

S Yoshida & Ye-Xiuyun 1991b Isolation of lactoperoxidase and lactoferrin from bovine milk acid whey by carboxymethyl cation exchange chromatography. Journal of Dairy Science 74 14391444

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Journal of Dairy Research
  • ISSN: 0022-0299
  • EISSN: 1469-7629
  • URL: /core/journals/journal-of-dairy-research
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